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A Universal Photochemical Method to Prepare Carbohydrate Sensors Based on Perfluorophenylazide Modified Polydopamine for Study of Carbohydrate-Lectin Interactions by QCM Biosensor

A universal photochemical method to prepare carbohydrate sensors based on perfluorophenylazide (PFPA) modified polydopamine (PDA) for the study of carbohydrate–lectin interactions by a quartz crystal microbalance (QCM) biosensor was developed. The PFPA was immobilized on PDA-coated gold sensors via...

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Autores principales: Guo, Lili, Chao, Shuang, Huang, Pei, Lv, Xiukai, Song, Quanquan, Wu, Chunli, Pei, Yuxin, Pei, Zhichao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631999/
https://www.ncbi.nlm.nih.gov/pubmed/31185633
http://dx.doi.org/10.3390/polym11061023
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author Guo, Lili
Chao, Shuang
Huang, Pei
Lv, Xiukai
Song, Quanquan
Wu, Chunli
Pei, Yuxin
Pei, Zhichao
author_facet Guo, Lili
Chao, Shuang
Huang, Pei
Lv, Xiukai
Song, Quanquan
Wu, Chunli
Pei, Yuxin
Pei, Zhichao
author_sort Guo, Lili
collection PubMed
description A universal photochemical method to prepare carbohydrate sensors based on perfluorophenylazide (PFPA) modified polydopamine (PDA) for the study of carbohydrate–lectin interactions by a quartz crystal microbalance (QCM) biosensor was developed. The PFPA was immobilized on PDA-coated gold sensors via Schiff base reactions. Upon light irradiation, the underivatized carbohydrates were inserted into the sensor surface, including mannose, galactose, fucose and N-acetylglucosamine (GlcNAc). Carbohydrate sensors were evaluated for the binding to a series of plant lectins. A kinetic study of the interactions between mannose and concanavalin A (Con A), fucose and Ulex europaeus agglutinin I (UEA-I) were performed. This method can eliminate the tedious modification of carbohydrates, improve the experimental efficiency, and reduce the experimental cost, which is of great significance for the development of QCM biosensors and the study of biomolecular interactions.
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spelling pubmed-66319992019-08-19 A Universal Photochemical Method to Prepare Carbohydrate Sensors Based on Perfluorophenylazide Modified Polydopamine for Study of Carbohydrate-Lectin Interactions by QCM Biosensor Guo, Lili Chao, Shuang Huang, Pei Lv, Xiukai Song, Quanquan Wu, Chunli Pei, Yuxin Pei, Zhichao Polymers (Basel) Article A universal photochemical method to prepare carbohydrate sensors based on perfluorophenylazide (PFPA) modified polydopamine (PDA) for the study of carbohydrate–lectin interactions by a quartz crystal microbalance (QCM) biosensor was developed. The PFPA was immobilized on PDA-coated gold sensors via Schiff base reactions. Upon light irradiation, the underivatized carbohydrates were inserted into the sensor surface, including mannose, galactose, fucose and N-acetylglucosamine (GlcNAc). Carbohydrate sensors were evaluated for the binding to a series of plant lectins. A kinetic study of the interactions between mannose and concanavalin A (Con A), fucose and Ulex europaeus agglutinin I (UEA-I) were performed. This method can eliminate the tedious modification of carbohydrates, improve the experimental efficiency, and reduce the experimental cost, which is of great significance for the development of QCM biosensors and the study of biomolecular interactions. MDPI 2019-06-10 /pmc/articles/PMC6631999/ /pubmed/31185633 http://dx.doi.org/10.3390/polym11061023 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guo, Lili
Chao, Shuang
Huang, Pei
Lv, Xiukai
Song, Quanquan
Wu, Chunli
Pei, Yuxin
Pei, Zhichao
A Universal Photochemical Method to Prepare Carbohydrate Sensors Based on Perfluorophenylazide Modified Polydopamine for Study of Carbohydrate-Lectin Interactions by QCM Biosensor
title A Universal Photochemical Method to Prepare Carbohydrate Sensors Based on Perfluorophenylazide Modified Polydopamine for Study of Carbohydrate-Lectin Interactions by QCM Biosensor
title_full A Universal Photochemical Method to Prepare Carbohydrate Sensors Based on Perfluorophenylazide Modified Polydopamine for Study of Carbohydrate-Lectin Interactions by QCM Biosensor
title_fullStr A Universal Photochemical Method to Prepare Carbohydrate Sensors Based on Perfluorophenylazide Modified Polydopamine for Study of Carbohydrate-Lectin Interactions by QCM Biosensor
title_full_unstemmed A Universal Photochemical Method to Prepare Carbohydrate Sensors Based on Perfluorophenylazide Modified Polydopamine for Study of Carbohydrate-Lectin Interactions by QCM Biosensor
title_short A Universal Photochemical Method to Prepare Carbohydrate Sensors Based on Perfluorophenylazide Modified Polydopamine for Study of Carbohydrate-Lectin Interactions by QCM Biosensor
title_sort universal photochemical method to prepare carbohydrate sensors based on perfluorophenylazide modified polydopamine for study of carbohydrate-lectin interactions by qcm biosensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631999/
https://www.ncbi.nlm.nih.gov/pubmed/31185633
http://dx.doi.org/10.3390/polym11061023
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