Cargando…

Mycobacterium avium subsp. paratuberculosis ELISA Responses in Milk Samples from Vaccinated and Nonvaccinated Dairy Goat Herds in The Netherlands

The aims of our study were to calculate the most appropriate cut-off value for milk samples in a serum-validated Mycobacterium avium subsp. paratuberculosis (MAP) ELISA and to analyze MAP ELISA responses in milk samples from vaccinated and nonvaccinated dairy goats in the Netherlands. Analyzed herds...

Descripción completa

Detalles Bibliográficos
Autores principales: Luttikholt, Saskia, Lievaart-Peterson, Karianne, Gonggrijp, Maaike, Aalberts, Marian, van Schaik, Gerdien, Vellema, Piet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6632119/
https://www.ncbi.nlm.nih.gov/pubmed/31234509
http://dx.doi.org/10.3390/vetsci6020058
_version_ 1783435673092489216
author Luttikholt, Saskia
Lievaart-Peterson, Karianne
Gonggrijp, Maaike
Aalberts, Marian
van Schaik, Gerdien
Vellema, Piet
author_facet Luttikholt, Saskia
Lievaart-Peterson, Karianne
Gonggrijp, Maaike
Aalberts, Marian
van Schaik, Gerdien
Vellema, Piet
author_sort Luttikholt, Saskia
collection PubMed
description The aims of our study were to calculate the most appropriate cut-off value for milk samples in a serum-validated Mycobacterium avium subsp. paratuberculosis (MAP) ELISA and to analyze MAP ELISA responses in milk samples from vaccinated and nonvaccinated dairy goats in the Netherlands. Analyzed herds were representative for location and herd size of dairy goat herds in the Netherlands. A significantly higher proportion of the analyzed 49 herds were organic as compared with the total Dutch dairy goat population. First, the MAP ELISA was optimized using 992 paired serum and milk samples. At a cut-off of 25 S/P%, the relative sensitivity (Se) was 58.4% (n = 992, 95% CI: 48.8%−67.6%) and relative specificity (Sp) was 98.5% (n = 992, 95% CI: 97.5%−99.2%), as compared to serum ELISA results. The percentage of positively tested herds was 78.2% (n = 49, 95% CI: 63.4%−88.1%). The percentage of positive milk samples per herd (n = 22) was on average 4.6% (median, min, and max of 4.7%, 0.0%, and 10.7%, respectively). Average age of ELISA-positive (3.2 years) and -negative goats (3.2 years) was not different. Significantly more vaccinated goats tested positive (6.7%) as compared with nonvaccinated goats (1.1%). This study shows that a high number of vaccinated and nonvaccinated commercial dairy goat herds in the Netherlands have MAP-ELISA-positive goats.
format Online
Article
Text
id pubmed-6632119
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-66321192019-08-19 Mycobacterium avium subsp. paratuberculosis ELISA Responses in Milk Samples from Vaccinated and Nonvaccinated Dairy Goat Herds in The Netherlands Luttikholt, Saskia Lievaart-Peterson, Karianne Gonggrijp, Maaike Aalberts, Marian van Schaik, Gerdien Vellema, Piet Vet Sci Article The aims of our study were to calculate the most appropriate cut-off value for milk samples in a serum-validated Mycobacterium avium subsp. paratuberculosis (MAP) ELISA and to analyze MAP ELISA responses in milk samples from vaccinated and nonvaccinated dairy goats in the Netherlands. Analyzed herds were representative for location and herd size of dairy goat herds in the Netherlands. A significantly higher proportion of the analyzed 49 herds were organic as compared with the total Dutch dairy goat population. First, the MAP ELISA was optimized using 992 paired serum and milk samples. At a cut-off of 25 S/P%, the relative sensitivity (Se) was 58.4% (n = 992, 95% CI: 48.8%−67.6%) and relative specificity (Sp) was 98.5% (n = 992, 95% CI: 97.5%−99.2%), as compared to serum ELISA results. The percentage of positively tested herds was 78.2% (n = 49, 95% CI: 63.4%−88.1%). The percentage of positive milk samples per herd (n = 22) was on average 4.6% (median, min, and max of 4.7%, 0.0%, and 10.7%, respectively). Average age of ELISA-positive (3.2 years) and -negative goats (3.2 years) was not different. Significantly more vaccinated goats tested positive (6.7%) as compared with nonvaccinated goats (1.1%). This study shows that a high number of vaccinated and nonvaccinated commercial dairy goat herds in the Netherlands have MAP-ELISA-positive goats. MDPI 2019-06-22 /pmc/articles/PMC6632119/ /pubmed/31234509 http://dx.doi.org/10.3390/vetsci6020058 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luttikholt, Saskia
Lievaart-Peterson, Karianne
Gonggrijp, Maaike
Aalberts, Marian
van Schaik, Gerdien
Vellema, Piet
Mycobacterium avium subsp. paratuberculosis ELISA Responses in Milk Samples from Vaccinated and Nonvaccinated Dairy Goat Herds in The Netherlands
title Mycobacterium avium subsp. paratuberculosis ELISA Responses in Milk Samples from Vaccinated and Nonvaccinated Dairy Goat Herds in The Netherlands
title_full Mycobacterium avium subsp. paratuberculosis ELISA Responses in Milk Samples from Vaccinated and Nonvaccinated Dairy Goat Herds in The Netherlands
title_fullStr Mycobacterium avium subsp. paratuberculosis ELISA Responses in Milk Samples from Vaccinated and Nonvaccinated Dairy Goat Herds in The Netherlands
title_full_unstemmed Mycobacterium avium subsp. paratuberculosis ELISA Responses in Milk Samples from Vaccinated and Nonvaccinated Dairy Goat Herds in The Netherlands
title_short Mycobacterium avium subsp. paratuberculosis ELISA Responses in Milk Samples from Vaccinated and Nonvaccinated Dairy Goat Herds in The Netherlands
title_sort mycobacterium avium subsp. paratuberculosis elisa responses in milk samples from vaccinated and nonvaccinated dairy goat herds in the netherlands
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6632119/
https://www.ncbi.nlm.nih.gov/pubmed/31234509
http://dx.doi.org/10.3390/vetsci6020058
work_keys_str_mv AT luttikholtsaskia mycobacteriumaviumsubspparatuberculosiselisaresponsesinmilksamplesfromvaccinatedandnonvaccinateddairygoatherdsinthenetherlands
AT lievaartpetersonkarianne mycobacteriumaviumsubspparatuberculosiselisaresponsesinmilksamplesfromvaccinatedandnonvaccinateddairygoatherdsinthenetherlands
AT gonggrijpmaaike mycobacteriumaviumsubspparatuberculosiselisaresponsesinmilksamplesfromvaccinatedandnonvaccinateddairygoatherdsinthenetherlands
AT aalbertsmarian mycobacteriumaviumsubspparatuberculosiselisaresponsesinmilksamplesfromvaccinatedandnonvaccinateddairygoatherdsinthenetherlands
AT vanschaikgerdien mycobacteriumaviumsubspparatuberculosiselisaresponsesinmilksamplesfromvaccinatedandnonvaccinateddairygoatherdsinthenetherlands
AT vellemapiet mycobacteriumaviumsubspparatuberculosiselisaresponsesinmilksamplesfromvaccinatedandnonvaccinateddairygoatherdsinthenetherlands