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Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis
The developmental pathway of the neural retina (NR) and retinal pigment epithelium (RPE) has been revealed by extensive research in mice. However, the molecular mechanisms underlying the development of the human NR and RPE, as well as the interactions between these two tissues, have not been well de...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6634428/ https://www.ncbi.nlm.nih.gov/pubmed/31269016 http://dx.doi.org/10.1371/journal.pbio.3000365 |
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author | Hu, Yuqiong Wang, Xiaoye Hu, Boqiang Mao, Yunuo Chen, Yidong Yan, Liying Yong, Jun Dong, Ji Wei, Yuan Wang, Wei Wen, Lu Qiao, Jie Tang, Fuchou |
author_facet | Hu, Yuqiong Wang, Xiaoye Hu, Boqiang Mao, Yunuo Chen, Yidong Yan, Liying Yong, Jun Dong, Ji Wei, Yuan Wang, Wei Wen, Lu Qiao, Jie Tang, Fuchou |
author_sort | Hu, Yuqiong |
collection | PubMed |
description | The developmental pathway of the neural retina (NR) and retinal pigment epithelium (RPE) has been revealed by extensive research in mice. However, the molecular mechanisms underlying the development of the human NR and RPE, as well as the interactions between these two tissues, have not been well defined. Here, we analyzed 2,421 individual cells from human fetal NR and RPE using single-cell RNA sequencing (RNA-seq) technique and revealed the tightly regulated spatiotemporal gene expression network of human retinal cells. We identified major cell classes of human fetal retina and potential crucial transcription factors for each cell class. We dissected the dynamic expression patterns of visual cycle– and ligand-receptor interaction–related genes in the RPE and NR. Moreover, we provided a map of disease-related genes for human fetal retinal cells and highlighted the importance of retinal progenitor cells as potential targets of inherited retinal diseases. Our findings captured the key in vivo features of the development of the human NR and RPE and offered insightful clues for further functional studies. |
format | Online Article Text |
id | pubmed-6634428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-66344282019-07-25 Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis Hu, Yuqiong Wang, Xiaoye Hu, Boqiang Mao, Yunuo Chen, Yidong Yan, Liying Yong, Jun Dong, Ji Wei, Yuan Wang, Wei Wen, Lu Qiao, Jie Tang, Fuchou PLoS Biol Methods and Resources The developmental pathway of the neural retina (NR) and retinal pigment epithelium (RPE) has been revealed by extensive research in mice. However, the molecular mechanisms underlying the development of the human NR and RPE, as well as the interactions between these two tissues, have not been well defined. Here, we analyzed 2,421 individual cells from human fetal NR and RPE using single-cell RNA sequencing (RNA-seq) technique and revealed the tightly regulated spatiotemporal gene expression network of human retinal cells. We identified major cell classes of human fetal retina and potential crucial transcription factors for each cell class. We dissected the dynamic expression patterns of visual cycle– and ligand-receptor interaction–related genes in the RPE and NR. Moreover, we provided a map of disease-related genes for human fetal retinal cells and highlighted the importance of retinal progenitor cells as potential targets of inherited retinal diseases. Our findings captured the key in vivo features of the development of the human NR and RPE and offered insightful clues for further functional studies. Public Library of Science 2019-07-03 /pmc/articles/PMC6634428/ /pubmed/31269016 http://dx.doi.org/10.1371/journal.pbio.3000365 Text en © 2019 Hu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Methods and Resources Hu, Yuqiong Wang, Xiaoye Hu, Boqiang Mao, Yunuo Chen, Yidong Yan, Liying Yong, Jun Dong, Ji Wei, Yuan Wang, Wei Wen, Lu Qiao, Jie Tang, Fuchou Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis |
title | Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis |
title_full | Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis |
title_fullStr | Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis |
title_full_unstemmed | Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis |
title_short | Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis |
title_sort | dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell rna-seq analysis |
topic | Methods and Resources |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6634428/ https://www.ncbi.nlm.nih.gov/pubmed/31269016 http://dx.doi.org/10.1371/journal.pbio.3000365 |
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