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Target Site Insensitivity Detection in Deltamethrin Resistant Culex pipiens Complex in Iran
BACKGROUND: Some mosquito species which belong to the Culex. pipiens complex are primary vectors for West Nile virus, Sindbis, Dirofilaria immitis, and many arboviruses. Knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) gene of Cx. pipiens that is inherited, is one of t...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635334/ https://www.ncbi.nlm.nih.gov/pubmed/31341851 |
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author | ZEIDABADINEZHAD, Reza VATANDOOST, Hassan ABAI, Mohammad Reza DINPARAST DJADID, Navid RAZ, Abbasali SEDAGHAT, Mohammad Mahdi OSHAGHI, Mohamad Ali RAEISI, Ahmad ADIBI, Neda |
author_facet | ZEIDABADINEZHAD, Reza VATANDOOST, Hassan ABAI, Mohammad Reza DINPARAST DJADID, Navid RAZ, Abbasali SEDAGHAT, Mohammad Mahdi OSHAGHI, Mohamad Ali RAEISI, Ahmad ADIBI, Neda |
author_sort | ZEIDABADINEZHAD, Reza |
collection | PubMed |
description | BACKGROUND: Some mosquito species which belong to the Culex. pipiens complex are primary vectors for West Nile virus, Sindbis, Dirofilaria immitis, and many arboviruses. Knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) gene of Cx. pipiens that is inherited, is one of the important threats for the efficacy of pyrethroids insecticides. Knockdown resistance (kdr) mutation, L1014F, is a well-defined mechanism of resistance to pyrethroids and DDT in many insect species. The aim of study was to determine the mechanisms of Insecticide resistance in this species METHODS: Specimens of Cx. pipiens, the major vector of West Nile virus, were obtained in Tehran, Iran by collecting larvae from polluted wastewater in Qarchak of Tehran. In 2016 Insecticide susceptibility tests were performed according to WHO methods with deltamethrin 0.05%. We focused on determination of this point mutation in the VGSC gene of Cx. pipiens by Real-time PCR. RESULTS: Our results revealed high levels of resistance to deltamethrin 0.05%. The lethal times i.e. LT(50) and LT(90) for deltamethrin were 2.1530 and 8.5117 h respectively. The result of Real-time PCR confirmed the presence of resistant genotype in all the members of tested population. This study is the first report on kdr genotyping of Cx. pipiens from Tehran and our results on the VGSC gene in position L1014F confirmed the TTA to TTT nucleotide change. CONCLUSION: This finding will provide a clue for management of insecticide resistance in mosquito which are vectors of arboviruses and decision for replacement of novel approach for vector control. |
format | Online Article Text |
id | pubmed-6635334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-66353342019-07-24 Target Site Insensitivity Detection in Deltamethrin Resistant Culex pipiens Complex in Iran ZEIDABADINEZHAD, Reza VATANDOOST, Hassan ABAI, Mohammad Reza DINPARAST DJADID, Navid RAZ, Abbasali SEDAGHAT, Mohammad Mahdi OSHAGHI, Mohamad Ali RAEISI, Ahmad ADIBI, Neda Iran J Public Health Original Article BACKGROUND: Some mosquito species which belong to the Culex. pipiens complex are primary vectors for West Nile virus, Sindbis, Dirofilaria immitis, and many arboviruses. Knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) gene of Cx. pipiens that is inherited, is one of the important threats for the efficacy of pyrethroids insecticides. Knockdown resistance (kdr) mutation, L1014F, is a well-defined mechanism of resistance to pyrethroids and DDT in many insect species. The aim of study was to determine the mechanisms of Insecticide resistance in this species METHODS: Specimens of Cx. pipiens, the major vector of West Nile virus, were obtained in Tehran, Iran by collecting larvae from polluted wastewater in Qarchak of Tehran. In 2016 Insecticide susceptibility tests were performed according to WHO methods with deltamethrin 0.05%. We focused on determination of this point mutation in the VGSC gene of Cx. pipiens by Real-time PCR. RESULTS: Our results revealed high levels of resistance to deltamethrin 0.05%. The lethal times i.e. LT(50) and LT(90) for deltamethrin were 2.1530 and 8.5117 h respectively. The result of Real-time PCR confirmed the presence of resistant genotype in all the members of tested population. This study is the first report on kdr genotyping of Cx. pipiens from Tehran and our results on the VGSC gene in position L1014F confirmed the TTA to TTT nucleotide change. CONCLUSION: This finding will provide a clue for management of insecticide resistance in mosquito which are vectors of arboviruses and decision for replacement of novel approach for vector control. Tehran University of Medical Sciences 2019-06 /pmc/articles/PMC6635334/ /pubmed/31341851 Text en Copyright© Iranian Public Health Association & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article ZEIDABADINEZHAD, Reza VATANDOOST, Hassan ABAI, Mohammad Reza DINPARAST DJADID, Navid RAZ, Abbasali SEDAGHAT, Mohammad Mahdi OSHAGHI, Mohamad Ali RAEISI, Ahmad ADIBI, Neda Target Site Insensitivity Detection in Deltamethrin Resistant Culex pipiens Complex in Iran |
title | Target Site Insensitivity Detection in Deltamethrin Resistant Culex pipiens Complex in Iran |
title_full | Target Site Insensitivity Detection in Deltamethrin Resistant Culex pipiens Complex in Iran |
title_fullStr | Target Site Insensitivity Detection in Deltamethrin Resistant Culex pipiens Complex in Iran |
title_full_unstemmed | Target Site Insensitivity Detection in Deltamethrin Resistant Culex pipiens Complex in Iran |
title_short | Target Site Insensitivity Detection in Deltamethrin Resistant Culex pipiens Complex in Iran |
title_sort | target site insensitivity detection in deltamethrin resistant culex pipiens complex in iran |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635334/ https://www.ncbi.nlm.nih.gov/pubmed/31341851 |
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