Cargando…
Solvent-Assisted Paper Spray Ionization Mass Spectrometry (SAPSI-MS) for the Analysis of Biomolecules and Biofluids
Paper Spray Ionization (PSI) is commonly applied for the analysis of small molecules, including drugs, metabolites, and pesticides in biological fluids, due to its high versatility, simplicity, and low costs. In this study, a new setup called Solvent Assisted Paper Spray Ionization (SAPSI), able to...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635430/ https://www.ncbi.nlm.nih.gov/pubmed/31311939 http://dx.doi.org/10.1038/s41598-019-45358-x |
_version_ | 1783435884253675520 |
---|---|
author | Riboni, Nicoló Quaranta, Alessandro Motwani, Hitesh V. Österlund, Nicklas Gräslund, Astrid Bianchi, Federica Ilag, Leopold L. |
author_facet | Riboni, Nicoló Quaranta, Alessandro Motwani, Hitesh V. Österlund, Nicklas Gräslund, Astrid Bianchi, Federica Ilag, Leopold L. |
author_sort | Riboni, Nicoló |
collection | PubMed |
description | Paper Spray Ionization (PSI) is commonly applied for the analysis of small molecules, including drugs, metabolites, and pesticides in biological fluids, due to its high versatility, simplicity, and low costs. In this study, a new setup called Solvent Assisted Paper Spray Ionization (SAPSI), able to increase data acquisition time, signal stability, and repeatability, is proposed to overcome common PSI drawbacks. The setup relies on an integrated solution to provide ionization potential and constant solvent flow to the paper tip. Specifically, the ion source was connected to the instrument fluidics along with the voltage supply systems, ensuring a close control over the ionization conditions. SAPSI was successfully applied for the analysis of different classes of biomolecules: amyloidogenic peptides, proteins, and N-glycans. The prolonged analysis time allowed real-time monitoring of processes taking places on the paper tip, such as amyloid peptides aggregation and disaggregation phenomena. The enhanced signal stability allowed to discriminate protein species characterized by different post translational modifications and adducts with electrophilic compounds, both in aqueous solutions and in biofluids, such as serum and cerebrospinal fluid, without any sample pretreatment. In the next future, application to clinical relevant modifications, could lead to the development of quick and cost-effective diagnostic tools. |
format | Online Article Text |
id | pubmed-6635430 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66354302019-07-24 Solvent-Assisted Paper Spray Ionization Mass Spectrometry (SAPSI-MS) for the Analysis of Biomolecules and Biofluids Riboni, Nicoló Quaranta, Alessandro Motwani, Hitesh V. Österlund, Nicklas Gräslund, Astrid Bianchi, Federica Ilag, Leopold L. Sci Rep Article Paper Spray Ionization (PSI) is commonly applied for the analysis of small molecules, including drugs, metabolites, and pesticides in biological fluids, due to its high versatility, simplicity, and low costs. In this study, a new setup called Solvent Assisted Paper Spray Ionization (SAPSI), able to increase data acquisition time, signal stability, and repeatability, is proposed to overcome common PSI drawbacks. The setup relies on an integrated solution to provide ionization potential and constant solvent flow to the paper tip. Specifically, the ion source was connected to the instrument fluidics along with the voltage supply systems, ensuring a close control over the ionization conditions. SAPSI was successfully applied for the analysis of different classes of biomolecules: amyloidogenic peptides, proteins, and N-glycans. The prolonged analysis time allowed real-time monitoring of processes taking places on the paper tip, such as amyloid peptides aggregation and disaggregation phenomena. The enhanced signal stability allowed to discriminate protein species characterized by different post translational modifications and adducts with electrophilic compounds, both in aqueous solutions and in biofluids, such as serum and cerebrospinal fluid, without any sample pretreatment. In the next future, application to clinical relevant modifications, could lead to the development of quick and cost-effective diagnostic tools. Nature Publishing Group UK 2019-07-16 /pmc/articles/PMC6635430/ /pubmed/31311939 http://dx.doi.org/10.1038/s41598-019-45358-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Riboni, Nicoló Quaranta, Alessandro Motwani, Hitesh V. Österlund, Nicklas Gräslund, Astrid Bianchi, Federica Ilag, Leopold L. Solvent-Assisted Paper Spray Ionization Mass Spectrometry (SAPSI-MS) for the Analysis of Biomolecules and Biofluids |
title | Solvent-Assisted Paper Spray Ionization Mass Spectrometry (SAPSI-MS) for the Analysis of Biomolecules and Biofluids |
title_full | Solvent-Assisted Paper Spray Ionization Mass Spectrometry (SAPSI-MS) for the Analysis of Biomolecules and Biofluids |
title_fullStr | Solvent-Assisted Paper Spray Ionization Mass Spectrometry (SAPSI-MS) for the Analysis of Biomolecules and Biofluids |
title_full_unstemmed | Solvent-Assisted Paper Spray Ionization Mass Spectrometry (SAPSI-MS) for the Analysis of Biomolecules and Biofluids |
title_short | Solvent-Assisted Paper Spray Ionization Mass Spectrometry (SAPSI-MS) for the Analysis of Biomolecules and Biofluids |
title_sort | solvent-assisted paper spray ionization mass spectrometry (sapsi-ms) for the analysis of biomolecules and biofluids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635430/ https://www.ncbi.nlm.nih.gov/pubmed/31311939 http://dx.doi.org/10.1038/s41598-019-45358-x |
work_keys_str_mv | AT riboninicolo solventassistedpapersprayionizationmassspectrometrysapsimsfortheanalysisofbiomoleculesandbiofluids AT quarantaalessandro solventassistedpapersprayionizationmassspectrometrysapsimsfortheanalysisofbiomoleculesandbiofluids AT motwanihiteshv solventassistedpapersprayionizationmassspectrometrysapsimsfortheanalysisofbiomoleculesandbiofluids AT osterlundnicklas solventassistedpapersprayionizationmassspectrometrysapsimsfortheanalysisofbiomoleculesandbiofluids AT graslundastrid solventassistedpapersprayionizationmassspectrometrysapsimsfortheanalysisofbiomoleculesandbiofluids AT bianchifederica solventassistedpapersprayionizationmassspectrometrysapsimsfortheanalysisofbiomoleculesandbiofluids AT ilagleopoldl solventassistedpapersprayionizationmassspectrometrysapsimsfortheanalysisofbiomoleculesandbiofluids |