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Highly selective reduced graphene oxide (rGO) sensor based on a peptide aptamer receptor for detecting explosives
An essential requirement for bio/chemical sensors and electronic nose systems is the ability to detect the intended target at room temperature with high selectivity. We report a reduced graphene oxide (rGO)-based gas sensor functionalized with a peptide receptor to detect dinitrotoluene (DNT), which...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635493/ https://www.ncbi.nlm.nih.gov/pubmed/31311944 http://dx.doi.org/10.1038/s41598-019-45936-z |
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author | Lee, Kyungjae Yoo, Yong Kyoung Chae, Myung-Sic Hwang, Kyo Seon Lee, Junwoo Kim, Hyungsuk Hur, Don Lee, Jeong Hoon |
author_facet | Lee, Kyungjae Yoo, Yong Kyoung Chae, Myung-Sic Hwang, Kyo Seon Lee, Junwoo Kim, Hyungsuk Hur, Don Lee, Jeong Hoon |
author_sort | Lee, Kyungjae |
collection | PubMed |
description | An essential requirement for bio/chemical sensors and electronic nose systems is the ability to detect the intended target at room temperature with high selectivity. We report a reduced graphene oxide (rGO)-based gas sensor functionalized with a peptide receptor to detect dinitrotoluene (DNT), which is a byproduct of trinitrotoluene (TNT). We fabricated the multi-arrayed rGO sensor using spin coating and a standard microfabrication technique. Subsequently, the rGO was subjected to photolithography and an etching process, after which we prepared the DNT-specific binding peptide (DNT-bp, sequence: His-Pro-Asn-Phe-Se r-Lys-Tyr-IleLeu-HisGln-Arg-Cys) and DNT non-specific binding peptide (DNT-nbp, sequence: Thr-Ser-Met-Leu-Leu-Met-Ser-Pro-Lys-His-Gln-Ala-Cys). These two peptides were prepared to function as highly specific and highly non-specific (for the control experiment) peptide receptors, respectively. By detecting the differential signals between the DNT-bp and DNT-nbp functionalized rGO sensor, we demonstrated the ability of 2,4-dinitrotoluene (DNT) targets to bind to DNT-specific binding peptide surfaces, showing good sensitivity and selectivity. The advantage of using the differential signal is that it eliminates unwanted electrical noise and/or environmental effects. We achieved sensitivity of 27 ± 2 × 10(−6) per part per billion (ppb) for the slope of resistance change versus DNT gas concentration of 80, 160, 240, 320, and 480 ppm, respectively. By sequentially flowing DNT vapor (320 ppb), acetone (100 ppm), toluene (1 ppm), and ethanol (100 ppm) onto the rGO sensors, the change in the signal of rGO in the presence of DNT gas is 6400 × 10(−6) per ppb whereas the signals from the other gases show no changes, representing highly selective performance. Using this platform, we were also able to regenerate the surface by simply purging with N(2). |
format | Online Article Text |
id | pubmed-6635493 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66354932019-07-24 Highly selective reduced graphene oxide (rGO) sensor based on a peptide aptamer receptor for detecting explosives Lee, Kyungjae Yoo, Yong Kyoung Chae, Myung-Sic Hwang, Kyo Seon Lee, Junwoo Kim, Hyungsuk Hur, Don Lee, Jeong Hoon Sci Rep Article An essential requirement for bio/chemical sensors and electronic nose systems is the ability to detect the intended target at room temperature with high selectivity. We report a reduced graphene oxide (rGO)-based gas sensor functionalized with a peptide receptor to detect dinitrotoluene (DNT), which is a byproduct of trinitrotoluene (TNT). We fabricated the multi-arrayed rGO sensor using spin coating and a standard microfabrication technique. Subsequently, the rGO was subjected to photolithography and an etching process, after which we prepared the DNT-specific binding peptide (DNT-bp, sequence: His-Pro-Asn-Phe-Se r-Lys-Tyr-IleLeu-HisGln-Arg-Cys) and DNT non-specific binding peptide (DNT-nbp, sequence: Thr-Ser-Met-Leu-Leu-Met-Ser-Pro-Lys-His-Gln-Ala-Cys). These two peptides were prepared to function as highly specific and highly non-specific (for the control experiment) peptide receptors, respectively. By detecting the differential signals between the DNT-bp and DNT-nbp functionalized rGO sensor, we demonstrated the ability of 2,4-dinitrotoluene (DNT) targets to bind to DNT-specific binding peptide surfaces, showing good sensitivity and selectivity. The advantage of using the differential signal is that it eliminates unwanted electrical noise and/or environmental effects. We achieved sensitivity of 27 ± 2 × 10(−6) per part per billion (ppb) for the slope of resistance change versus DNT gas concentration of 80, 160, 240, 320, and 480 ppm, respectively. By sequentially flowing DNT vapor (320 ppb), acetone (100 ppm), toluene (1 ppm), and ethanol (100 ppm) onto the rGO sensors, the change in the signal of rGO in the presence of DNT gas is 6400 × 10(−6) per ppb whereas the signals from the other gases show no changes, representing highly selective performance. Using this platform, we were also able to regenerate the surface by simply purging with N(2). Nature Publishing Group UK 2019-07-16 /pmc/articles/PMC6635493/ /pubmed/31311944 http://dx.doi.org/10.1038/s41598-019-45936-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lee, Kyungjae Yoo, Yong Kyoung Chae, Myung-Sic Hwang, Kyo Seon Lee, Junwoo Kim, Hyungsuk Hur, Don Lee, Jeong Hoon Highly selective reduced graphene oxide (rGO) sensor based on a peptide aptamer receptor for detecting explosives |
title | Highly selective reduced graphene oxide (rGO) sensor based on a peptide aptamer receptor for detecting explosives |
title_full | Highly selective reduced graphene oxide (rGO) sensor based on a peptide aptamer receptor for detecting explosives |
title_fullStr | Highly selective reduced graphene oxide (rGO) sensor based on a peptide aptamer receptor for detecting explosives |
title_full_unstemmed | Highly selective reduced graphene oxide (rGO) sensor based on a peptide aptamer receptor for detecting explosives |
title_short | Highly selective reduced graphene oxide (rGO) sensor based on a peptide aptamer receptor for detecting explosives |
title_sort | highly selective reduced graphene oxide (rgo) sensor based on a peptide aptamer receptor for detecting explosives |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635493/ https://www.ncbi.nlm.nih.gov/pubmed/31311944 http://dx.doi.org/10.1038/s41598-019-45936-z |
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