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Point-of-care measurements reveal release of purines into venous blood of stroke patients
Stroke is a leading cause of death and disability. Here, we examine whether point-of-care measurement of the purines, adenosine, inosine and hypoxanthine, which are downstream metabolites of ATP, has potential to assist the diagnosis of stroke. In a prospective observational study, patients who were...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635545/ https://www.ncbi.nlm.nih.gov/pubmed/30859371 http://dx.doi.org/10.1007/s11302-019-09647-4 |
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author | Dale, Nicholas Tian, Faming Sagoo, Ravjit Phillips, Norman Imray, Chris Roffe, Christine |
author_facet | Dale, Nicholas Tian, Faming Sagoo, Ravjit Phillips, Norman Imray, Chris Roffe, Christine |
author_sort | Dale, Nicholas |
collection | PubMed |
description | Stroke is a leading cause of death and disability. Here, we examine whether point-of-care measurement of the purines, adenosine, inosine and hypoxanthine, which are downstream metabolites of ATP, has potential to assist the diagnosis of stroke. In a prospective observational study, patients who were suspected of having had a stroke, within 4.5 h of symptom onset and still displaying focal neurological symptoms at admission, were recruited. Clinical research staff in the Emergency Departments of two hospitals used a prototype biosensor array, SMARTCap, to measure the purines in the venous blood of stroke patients and healthy controls. In controls, the baseline purines were 7.1 ± (SD) 4.2 μM (n = 52), while in stroke patients, they were 11.6 ± 8.9 μM (n = 76). Using the National Institutes for Stoke Scale (NIHSS) to band the severity of stroke, we found that minor, moderate and severe strokes all gave significant elevation of blood purines above the controls. The purine levels fall over 24 h. This was most marked for patients with haemorrhagic strokes (5.1 ± 3.6 μM, n = 9 after 24 h). The purine levels measured on admission show a significant correlation with the volume of affected brain tissue determined by medical imaging in patients who had not received thrombolysis or mechanical thrombectomy. ClinicalTrials.gov Identifier: NCT02308605 |
format | Online Article Text |
id | pubmed-6635545 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-66355452019-07-31 Point-of-care measurements reveal release of purines into venous blood of stroke patients Dale, Nicholas Tian, Faming Sagoo, Ravjit Phillips, Norman Imray, Chris Roffe, Christine Purinergic Signal Original Article Stroke is a leading cause of death and disability. Here, we examine whether point-of-care measurement of the purines, adenosine, inosine and hypoxanthine, which are downstream metabolites of ATP, has potential to assist the diagnosis of stroke. In a prospective observational study, patients who were suspected of having had a stroke, within 4.5 h of symptom onset and still displaying focal neurological symptoms at admission, were recruited. Clinical research staff in the Emergency Departments of two hospitals used a prototype biosensor array, SMARTCap, to measure the purines in the venous blood of stroke patients and healthy controls. In controls, the baseline purines were 7.1 ± (SD) 4.2 μM (n = 52), while in stroke patients, they were 11.6 ± 8.9 μM (n = 76). Using the National Institutes for Stoke Scale (NIHSS) to band the severity of stroke, we found that minor, moderate and severe strokes all gave significant elevation of blood purines above the controls. The purine levels fall over 24 h. This was most marked for patients with haemorrhagic strokes (5.1 ± 3.6 μM, n = 9 after 24 h). The purine levels measured on admission show a significant correlation with the volume of affected brain tissue determined by medical imaging in patients who had not received thrombolysis or mechanical thrombectomy. ClinicalTrials.gov Identifier: NCT02308605 Springer Netherlands 2019-03-12 2019-06 /pmc/articles/PMC6635545/ /pubmed/30859371 http://dx.doi.org/10.1007/s11302-019-09647-4 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Dale, Nicholas Tian, Faming Sagoo, Ravjit Phillips, Norman Imray, Chris Roffe, Christine Point-of-care measurements reveal release of purines into venous blood of stroke patients |
title | Point-of-care measurements reveal release of purines into venous blood of stroke patients |
title_full | Point-of-care measurements reveal release of purines into venous blood of stroke patients |
title_fullStr | Point-of-care measurements reveal release of purines into venous blood of stroke patients |
title_full_unstemmed | Point-of-care measurements reveal release of purines into venous blood of stroke patients |
title_short | Point-of-care measurements reveal release of purines into venous blood of stroke patients |
title_sort | point-of-care measurements reveal release of purines into venous blood of stroke patients |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635545/ https://www.ncbi.nlm.nih.gov/pubmed/30859371 http://dx.doi.org/10.1007/s11302-019-09647-4 |
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