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Surface Exclusion Revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20
During conjugation a genetic element is transferred from a bacterial donor to a recipient cell via a connecting channel. It is the major route responsible for the spread of antibiotic resistance. Conjugative elements can contain exclusion system(s) that inhibit its transfer to a cell already harbori...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635565/ https://www.ncbi.nlm.nih.gov/pubmed/31354647 http://dx.doi.org/10.3389/fmicb.2019.01502 |
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author | Gago-Córdoba, César Val-Calvo, Jorge Miguel-Arribas, Andrés Serrano, Ester Singh, Praveen K. Abia, David Wu, Ling Juan Meijer, Wilfried J. J. |
author_facet | Gago-Córdoba, César Val-Calvo, Jorge Miguel-Arribas, Andrés Serrano, Ester Singh, Praveen K. Abia, David Wu, Ling Juan Meijer, Wilfried J. J. |
author_sort | Gago-Córdoba, César |
collection | PubMed |
description | During conjugation a genetic element is transferred from a bacterial donor to a recipient cell via a connecting channel. It is the major route responsible for the spread of antibiotic resistance. Conjugative elements can contain exclusion system(s) that inhibit its transfer to a cell already harboring the element. Our limited knowledge on exclusion systems is mainly based on plasmids of Gram-negative bacteria. Here we studied the conjugative plasmid pLS20 of the Gram-positive Bacillus subtilis. We demonstrate that pLS20 contains an exclusion system and identified the single gene responsible for exclusion, named ses(pLS20), which is embedded in the conjugation operon. Ses(pLS20) is the founding member of a novel family of surface exclusion proteins encoded by conjugative elements of Gram-positive origin. We show that the extent of surface exclusion correlates with the level of ses(pLS20) expression, and that ses(pLS20) is expressed at basal low-levels in all donor cells but becomes highly expressed in conjugating cells. Accordingly, the transfer of pLS20 from a conjugation-primed donor cell to an un-primed or conjugation-primed donor is inhibited moderately and very efficiently, respectively. The consequences of this differential regulation, which appears to be a conserved feature of surface exclusion systems of Gram-positive and Gram-negative origin, are discussed. |
format | Online Article Text |
id | pubmed-6635565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66355652019-07-26 Surface Exclusion Revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20 Gago-Córdoba, César Val-Calvo, Jorge Miguel-Arribas, Andrés Serrano, Ester Singh, Praveen K. Abia, David Wu, Ling Juan Meijer, Wilfried J. J. Front Microbiol Microbiology During conjugation a genetic element is transferred from a bacterial donor to a recipient cell via a connecting channel. It is the major route responsible for the spread of antibiotic resistance. Conjugative elements can contain exclusion system(s) that inhibit its transfer to a cell already harboring the element. Our limited knowledge on exclusion systems is mainly based on plasmids of Gram-negative bacteria. Here we studied the conjugative plasmid pLS20 of the Gram-positive Bacillus subtilis. We demonstrate that pLS20 contains an exclusion system and identified the single gene responsible for exclusion, named ses(pLS20), which is embedded in the conjugation operon. Ses(pLS20) is the founding member of a novel family of surface exclusion proteins encoded by conjugative elements of Gram-positive origin. We show that the extent of surface exclusion correlates with the level of ses(pLS20) expression, and that ses(pLS20) is expressed at basal low-levels in all donor cells but becomes highly expressed in conjugating cells. Accordingly, the transfer of pLS20 from a conjugation-primed donor cell to an un-primed or conjugation-primed donor is inhibited moderately and very efficiently, respectively. The consequences of this differential regulation, which appears to be a conserved feature of surface exclusion systems of Gram-positive and Gram-negative origin, are discussed. Frontiers Media S.A. 2019-07-10 /pmc/articles/PMC6635565/ /pubmed/31354647 http://dx.doi.org/10.3389/fmicb.2019.01502 Text en Copyright © 2019 Gago-Córdoba, Val-Calvo, Miguel-Arribas, Serrano, Singh, Abia, Wu and Meijer. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Gago-Córdoba, César Val-Calvo, Jorge Miguel-Arribas, Andrés Serrano, Ester Singh, Praveen K. Abia, David Wu, Ling Juan Meijer, Wilfried J. J. Surface Exclusion Revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20 |
title | Surface Exclusion Revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20 |
title_full | Surface Exclusion Revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20 |
title_fullStr | Surface Exclusion Revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20 |
title_full_unstemmed | Surface Exclusion Revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20 |
title_short | Surface Exclusion Revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20 |
title_sort | surface exclusion revisited: function related to differential expression of the surface exclusion system of bacillus subtilis plasmid pls20 |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635565/ https://www.ncbi.nlm.nih.gov/pubmed/31354647 http://dx.doi.org/10.3389/fmicb.2019.01502 |
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