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Influence of bacterial burden on meibomian gland dysfunction and ocular surface disease

PURPOSE: Bacterial burden on the eyelid margin and within meibomian glands was evaluated for influence on specific ocular surface disease (OSD) markers across the meibomian gland dysfunction (MGD) spectrum. METHODS: In this prospective, observational, single-center study, 40 patients were divided in...

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Autores principales: Nattis, Alanna, Perry, Henry D, Rosenberg, Eric D, Donnenfeld, Eric D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635833/
https://www.ncbi.nlm.nih.gov/pubmed/31371918
http://dx.doi.org/10.2147/OPTH.S215071
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author Nattis, Alanna
Perry, Henry D
Rosenberg, Eric D
Donnenfeld, Eric D
author_facet Nattis, Alanna
Perry, Henry D
Rosenberg, Eric D
Donnenfeld, Eric D
author_sort Nattis, Alanna
collection PubMed
description PURPOSE: Bacterial burden on the eyelid margin and within meibomian glands was evaluated for influence on specific ocular surface disease (OSD) markers across the meibomian gland dysfunction (MGD) spectrum. METHODS: In this prospective, observational, single-center study, 40 patients were divided into 4 equal groups of 10 that encompassed increasingly worse MGD/OSD categories. All patients answered the standard Ocular Surface Disease Index questionnaire, and underwent tear osmolarity testing (TOT), Schirmer 1, matrix metalloproteinase 9 (MMP-9) testing, meibography, and lissamine green staining. Cultures of eyelid margins and meibomian gland secretions were directly plated on blood, chocolate, and Sabouraud agar; smears were sent for gram and Papinicolau evaluation. RESULTS: Mean patient age was 55.25±17.22 years; there were 10 males and 30 females. TOT and MMP-9 testing were similar across groups. Culture positivity was 62.5% for right eyes, 70% for left eyes, and was not statistically different across groups (for both eyelid margin and meibomian glands). The majority of cultures were positive for coagulase-negative staphylococcus (CNS). CONCLUSION: This study is in concordance with others, citing the predominance of CNS within the biofilm of both “normal” and clinically significant MGD/OSD patients. Our study exemplifies that symptoms of OSD do not necessarily correlate with degree of clinical exam findings, nor culture positivity. These results argue that bacterial burden should be reconsidered as a direct risk factor and treatment target for MGD/OSD patients.
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spelling pubmed-66358332019-08-01 Influence of bacterial burden on meibomian gland dysfunction and ocular surface disease Nattis, Alanna Perry, Henry D Rosenberg, Eric D Donnenfeld, Eric D Clin Ophthalmol Original Research PURPOSE: Bacterial burden on the eyelid margin and within meibomian glands was evaluated for influence on specific ocular surface disease (OSD) markers across the meibomian gland dysfunction (MGD) spectrum. METHODS: In this prospective, observational, single-center study, 40 patients were divided into 4 equal groups of 10 that encompassed increasingly worse MGD/OSD categories. All patients answered the standard Ocular Surface Disease Index questionnaire, and underwent tear osmolarity testing (TOT), Schirmer 1, matrix metalloproteinase 9 (MMP-9) testing, meibography, and lissamine green staining. Cultures of eyelid margins and meibomian gland secretions were directly plated on blood, chocolate, and Sabouraud agar; smears were sent for gram and Papinicolau evaluation. RESULTS: Mean patient age was 55.25±17.22 years; there were 10 males and 30 females. TOT and MMP-9 testing were similar across groups. Culture positivity was 62.5% for right eyes, 70% for left eyes, and was not statistically different across groups (for both eyelid margin and meibomian glands). The majority of cultures were positive for coagulase-negative staphylococcus (CNS). CONCLUSION: This study is in concordance with others, citing the predominance of CNS within the biofilm of both “normal” and clinically significant MGD/OSD patients. Our study exemplifies that symptoms of OSD do not necessarily correlate with degree of clinical exam findings, nor culture positivity. These results argue that bacterial burden should be reconsidered as a direct risk factor and treatment target for MGD/OSD patients. Dove 2019-07-12 /pmc/articles/PMC6635833/ /pubmed/31371918 http://dx.doi.org/10.2147/OPTH.S215071 Text en © 2019 Nattis et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Nattis, Alanna
Perry, Henry D
Rosenberg, Eric D
Donnenfeld, Eric D
Influence of bacterial burden on meibomian gland dysfunction and ocular surface disease
title Influence of bacterial burden on meibomian gland dysfunction and ocular surface disease
title_full Influence of bacterial burden on meibomian gland dysfunction and ocular surface disease
title_fullStr Influence of bacterial burden on meibomian gland dysfunction and ocular surface disease
title_full_unstemmed Influence of bacterial burden on meibomian gland dysfunction and ocular surface disease
title_short Influence of bacterial burden on meibomian gland dysfunction and ocular surface disease
title_sort influence of bacterial burden on meibomian gland dysfunction and ocular surface disease
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635833/
https://www.ncbi.nlm.nih.gov/pubmed/31371918
http://dx.doi.org/10.2147/OPTH.S215071
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