Cargando…
Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment
The carboxysome is a complex, proteinaceous organelle that plays essential roles in carbon assimilation in cyanobacteria and chemoautotrophs. It comprises hundreds of protein homologs that self-assemble in space to form an icosahedral structure. Despite its significance in enhancing CO(2) fixation a...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Plant Biologists
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635877/ https://www.ncbi.nlm.nih.gov/pubmed/31048338 http://dx.doi.org/10.1105/tpc.18.00787 |
_version_ | 1783435962413481984 |
---|---|
author | Sun, Yaqi Wollman, Adam J. M. Huang, Fang Leake, Mark C. Liu, Lu-Ning |
author_facet | Sun, Yaqi Wollman, Adam J. M. Huang, Fang Leake, Mark C. Liu, Lu-Ning |
author_sort | Sun, Yaqi |
collection | PubMed |
description | The carboxysome is a complex, proteinaceous organelle that plays essential roles in carbon assimilation in cyanobacteria and chemoautotrophs. It comprises hundreds of protein homologs that self-assemble in space to form an icosahedral structure. Despite its significance in enhancing CO(2) fixation and potentials in bioengineering applications, the formation of carboxysomes and their structural composition, stoichiometry, and adaptation to cope with environmental changes remain unclear. Here we use live-cell single-molecule fluorescence microscopy, coupled with confocal and electron microscopy, to decipher the absolute protein stoichiometry and organizational variability of single β-carboxysomes in the model cyanobacterium Synechococcus elongatus PCC7942. We determine the physiological abundance of individual building blocks within the icosahedral carboxysome. We further find that the protein stoichiometry, diameter, localization, and mobility patterns of carboxysomes in cells depend sensitively on the microenvironmental levels of CO(2) and light intensity during cell growth, revealing cellular strategies of dynamic regulation. These findings, also applicable to other bacterial microcompartments and macromolecular self-assembling systems, advance our knowledge of the principles that mediate carboxysome formation and structural modulation. It will empower rational design and construction of entire functional metabolic factories in heterologous organisms, for example crop plants, to boost photosynthesis and agricultural productivity. |
format | Online Article Text |
id | pubmed-6635877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Society of Plant Biologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-66358772019-07-22 Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment Sun, Yaqi Wollman, Adam J. M. Huang, Fang Leake, Mark C. Liu, Lu-Ning Plant Cell Research Articles The carboxysome is a complex, proteinaceous organelle that plays essential roles in carbon assimilation in cyanobacteria and chemoautotrophs. It comprises hundreds of protein homologs that self-assemble in space to form an icosahedral structure. Despite its significance in enhancing CO(2) fixation and potentials in bioengineering applications, the formation of carboxysomes and their structural composition, stoichiometry, and adaptation to cope with environmental changes remain unclear. Here we use live-cell single-molecule fluorescence microscopy, coupled with confocal and electron microscopy, to decipher the absolute protein stoichiometry and organizational variability of single β-carboxysomes in the model cyanobacterium Synechococcus elongatus PCC7942. We determine the physiological abundance of individual building blocks within the icosahedral carboxysome. We further find that the protein stoichiometry, diameter, localization, and mobility patterns of carboxysomes in cells depend sensitively on the microenvironmental levels of CO(2) and light intensity during cell growth, revealing cellular strategies of dynamic regulation. These findings, also applicable to other bacterial microcompartments and macromolecular self-assembling systems, advance our knowledge of the principles that mediate carboxysome formation and structural modulation. It will empower rational design and construction of entire functional metabolic factories in heterologous organisms, for example crop plants, to boost photosynthesis and agricultural productivity. American Society of Plant Biologists 2019-07 2019-05-02 /pmc/articles/PMC6635877/ /pubmed/31048338 http://dx.doi.org/10.1105/tpc.18.00787 Text en © 2019 The author(s). All rights reserved. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Sun, Yaqi Wollman, Adam J. M. Huang, Fang Leake, Mark C. Liu, Lu-Ning Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment |
title | Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment |
title_full | Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment |
title_fullStr | Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment |
title_full_unstemmed | Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment |
title_short | Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment |
title_sort | single-organelle quantification reveals stoichiometric and structural variability of carboxysomes dependent on the environment |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635877/ https://www.ncbi.nlm.nih.gov/pubmed/31048338 http://dx.doi.org/10.1105/tpc.18.00787 |
work_keys_str_mv | AT sunyaqi singleorganellequantificationrevealsstoichiometricandstructuralvariabilityofcarboxysomesdependentontheenvironment AT wollmanadamjm singleorganellequantificationrevealsstoichiometricandstructuralvariabilityofcarboxysomesdependentontheenvironment AT huangfang singleorganellequantificationrevealsstoichiometricandstructuralvariabilityofcarboxysomesdependentontheenvironment AT leakemarkc singleorganellequantificationrevealsstoichiometricandstructuralvariabilityofcarboxysomesdependentontheenvironment AT liuluning singleorganellequantificationrevealsstoichiometricandstructuralvariabilityofcarboxysomesdependentontheenvironment |