Development of 17 microsatellite markers in the federally endangered species Liatris helleri (Asteraceae)

PREMISE: Microsatellite markers were developed in the federally endangered species Liatris helleri (Asteraceae) to evaluate species boundaries with closely related congeners within the genus. METHODS AND RESULTS: Using Illumina data, 17 primer pairs were developed in populations of L. helleri. The p...

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Detalles Bibliográficos
Autores principales: Clark, Logan C., Gaglianese‐Woody, Morgan R., Estep, Matt C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Primer Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6636620/
https://www.ncbi.nlm.nih.gov/pubmed/31346504
http://dx.doi.org/10.1002/aps3.1250
Descripción
Sumario:PREMISE: Microsatellite markers were developed in the federally endangered species Liatris helleri (Asteraceae) to evaluate species boundaries with closely related congeners within the genus. METHODS AND RESULTS: Using Illumina data, 17 primer pairs were developed in populations of L. helleri. The primers amplified motifs from tri‐ to hexanucleotide repeats with one to 17 alleles per locus. Primers were also tested for cross‐amplification in L. aspera, L. microcephala, and L. pycnostachya. CONCLUSIONS: The developed primers for L. helleri serve as a novel genetic tool for future investigations in this genus, allowing for more explicit species delineation as well as population genetic analyses.

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