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Measuring vision using innate behaviours in mice with intact and impaired retina function
Measuring vision in rodents is a critical step for understanding vision, improving models of human disease, and developing therapies. Established behavioural tests for perceptual vision, such as the visual water task, rely on learning. The learning process, while effective for sighted animals, can b...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637134/ https://www.ncbi.nlm.nih.gov/pubmed/31316114 http://dx.doi.org/10.1038/s41598-019-46836-y |
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author | Storchi, R. Rodgers, J. Gracey, M. Martial, F. P. Wynne, J. Ryan, S. Twining, C. J. Cootes, T. F. Killick, R. Lucas, R. J. |
author_facet | Storchi, R. Rodgers, J. Gracey, M. Martial, F. P. Wynne, J. Ryan, S. Twining, C. J. Cootes, T. F. Killick, R. Lucas, R. J. |
author_sort | Storchi, R. |
collection | PubMed |
description | Measuring vision in rodents is a critical step for understanding vision, improving models of human disease, and developing therapies. Established behavioural tests for perceptual vision, such as the visual water task, rely on learning. The learning process, while effective for sighted animals, can be laborious and stressful in animals with impaired vision, requiring long periods of training. Current tests that that do not require training are based on sub-conscious, reflex responses (e.g. optokinetic nystagmus) that don’t require involvement of visual cortex and higher order thalamic nuclei. A potential alternative for measuring vision relies on using visually guided innate defensive responses, such as escape or freeze, that involve cortical and thalamic circuits. In this study we address this possibility in mice with intact and degenerate retinas. We first develop automatic methods to detect behavioural responses based on high dimensional tracking and changepoint detection of behavioural time series. Using those methods, we show that visually guided innate responses can be elicited using parametisable stimuli, and applied to describing the limits of visual acuity in healthy animals and discriminating degrees of visual dysfunction in mouse models of retinal degeneration. |
format | Online Article Text |
id | pubmed-6637134 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66371342019-07-25 Measuring vision using innate behaviours in mice with intact and impaired retina function Storchi, R. Rodgers, J. Gracey, M. Martial, F. P. Wynne, J. Ryan, S. Twining, C. J. Cootes, T. F. Killick, R. Lucas, R. J. Sci Rep Article Measuring vision in rodents is a critical step for understanding vision, improving models of human disease, and developing therapies. Established behavioural tests for perceptual vision, such as the visual water task, rely on learning. The learning process, while effective for sighted animals, can be laborious and stressful in animals with impaired vision, requiring long periods of training. Current tests that that do not require training are based on sub-conscious, reflex responses (e.g. optokinetic nystagmus) that don’t require involvement of visual cortex and higher order thalamic nuclei. A potential alternative for measuring vision relies on using visually guided innate defensive responses, such as escape or freeze, that involve cortical and thalamic circuits. In this study we address this possibility in mice with intact and degenerate retinas. We first develop automatic methods to detect behavioural responses based on high dimensional tracking and changepoint detection of behavioural time series. Using those methods, we show that visually guided innate responses can be elicited using parametisable stimuli, and applied to describing the limits of visual acuity in healthy animals and discriminating degrees of visual dysfunction in mouse models of retinal degeneration. Nature Publishing Group UK 2019-07-17 /pmc/articles/PMC6637134/ /pubmed/31316114 http://dx.doi.org/10.1038/s41598-019-46836-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Storchi, R. Rodgers, J. Gracey, M. Martial, F. P. Wynne, J. Ryan, S. Twining, C. J. Cootes, T. F. Killick, R. Lucas, R. J. Measuring vision using innate behaviours in mice with intact and impaired retina function |
title | Measuring vision using innate behaviours in mice with intact and impaired retina function |
title_full | Measuring vision using innate behaviours in mice with intact and impaired retina function |
title_fullStr | Measuring vision using innate behaviours in mice with intact and impaired retina function |
title_full_unstemmed | Measuring vision using innate behaviours in mice with intact and impaired retina function |
title_short | Measuring vision using innate behaviours in mice with intact and impaired retina function |
title_sort | measuring vision using innate behaviours in mice with intact and impaired retina function |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637134/ https://www.ncbi.nlm.nih.gov/pubmed/31316114 http://dx.doi.org/10.1038/s41598-019-46836-y |
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