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Pooled library screening with multiplexed Cpf1 library
Capitalizing on the inherent multiplexing capability of AsCpf1, we developed a multiplexed, high-throughput screening strategy that minimizes library size without sacrificing gene targeting efficiency. We demonstrated that AsCpf1 can be used for functional genomics screenings and that an AsCpf1-base...
Autores principales: | Liu, Jintan, Srinivasan, Sanjana, Li, Chieh-Yuan, Ho, I-Lin, Rose, Johnathon, Shaheen, MennatAllah, Wang, Gang, Yao, Wantong, Deem, Angela, Bristow, Chris, Hart, Traver, Draetta, Giulio |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637147/ https://www.ncbi.nlm.nih.gov/pubmed/31316073 http://dx.doi.org/10.1038/s41467-019-10963-x |
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