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Cryo-EM and directed evolution reveal how Arabidopsis nitrilase specificity is influenced by its quaternary structure
Nitrilases are helical enzymes that convert nitriles to acids and/or amides. All plants have a nitrilase 4 homolog specific for ß-cyanoalanine, while in some plants neofunctionalization has produced nitrilases with altered specificity. Plant nitrilase substrate size and specificity correlate with he...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637149/ https://www.ncbi.nlm.nih.gov/pubmed/31341959 http://dx.doi.org/10.1038/s42003-019-0505-4 |