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SPR signals enhancement by gold nanorods for cell surface marker detection

[Image: see text] Introduction: The detection of micrometer-sized particles like cells is limited by surface plasmon resonance (SPR) biosensors because of having a depth of evanescent wave <500 nm. In this study, for the first time, we exhibited the use of streptavidin-functionalized gold nanorod...

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Autores principales: Fathi, Farzaneh, Jalili, Roghayeh, Amjadi, Mohammad, Rashidi, Mohammad-Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637213/
https://www.ncbi.nlm.nih.gov/pubmed/31334038
http://dx.doi.org/10.15171/bi.2019.10
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author Fathi, Farzaneh
Jalili, Roghayeh
Amjadi, Mohammad
Rashidi, Mohammad-Reza
author_facet Fathi, Farzaneh
Jalili, Roghayeh
Amjadi, Mohammad
Rashidi, Mohammad-Reza
author_sort Fathi, Farzaneh
collection PubMed
description [Image: see text] Introduction: The detection of micrometer-sized particles like cells is limited by surface plasmon resonance (SPR) biosensors because of having a depth of evanescent wave <500 nm. In this study, for the first time, we exhibited the use of streptavidin-functionalized gold nanorods (GNRs) as intensification labels for detection of cell surface markers in SPR-based biosensors. Methods: The GNRs (ʎ max: 735 nm) were modified with streptavidin using EDC/NHS coupling method and human umbilical vein endothelial cells (HUVECs) were selected as the cell model for detecting VE-cadherin on cell surface using real-time SPR device in the 785 nm wavelength of the laser source. Results: The investigations revealed that the plasmonic field extension produced from the gold layer and GNRs resulted in multiple enhancement of SPR signals when the wavelength of laser source in SPR instrument was matched with the wavelength of maximum absorbance in GNRs. Moreover, the results showed that the growth of ∆RU value in specific and non-specific bindings for various cell number injections were produced with increasing the cell number. Conclusion: The results displayed that cell detection can be performed in real- time form without any need to a time-consuming process used in conventional methods like immunocytochemistry, flow cytometry, and western blotting.
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spelling pubmed-66372132019-07-22 SPR signals enhancement by gold nanorods for cell surface marker detection Fathi, Farzaneh Jalili, Roghayeh Amjadi, Mohammad Rashidi, Mohammad-Reza Bioimpacts Original Research [Image: see text] Introduction: The detection of micrometer-sized particles like cells is limited by surface plasmon resonance (SPR) biosensors because of having a depth of evanescent wave <500 nm. In this study, for the first time, we exhibited the use of streptavidin-functionalized gold nanorods (GNRs) as intensification labels for detection of cell surface markers in SPR-based biosensors. Methods: The GNRs (ʎ max: 735 nm) were modified with streptavidin using EDC/NHS coupling method and human umbilical vein endothelial cells (HUVECs) were selected as the cell model for detecting VE-cadherin on cell surface using real-time SPR device in the 785 nm wavelength of the laser source. Results: The investigations revealed that the plasmonic field extension produced from the gold layer and GNRs resulted in multiple enhancement of SPR signals when the wavelength of laser source in SPR instrument was matched with the wavelength of maximum absorbance in GNRs. Moreover, the results showed that the growth of ∆RU value in specific and non-specific bindings for various cell number injections were produced with increasing the cell number. Conclusion: The results displayed that cell detection can be performed in real- time form without any need to a time-consuming process used in conventional methods like immunocytochemistry, flow cytometry, and western blotting. Tabriz University of Medical Sciences 2019 2018-10-20 /pmc/articles/PMC6637213/ /pubmed/31334038 http://dx.doi.org/10.15171/bi.2019.10 Text en © 2019 The Author(s) This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Research
Fathi, Farzaneh
Jalili, Roghayeh
Amjadi, Mohammad
Rashidi, Mohammad-Reza
SPR signals enhancement by gold nanorods for cell surface marker detection
title SPR signals enhancement by gold nanorods for cell surface marker detection
title_full SPR signals enhancement by gold nanorods for cell surface marker detection
title_fullStr SPR signals enhancement by gold nanorods for cell surface marker detection
title_full_unstemmed SPR signals enhancement by gold nanorods for cell surface marker detection
title_short SPR signals enhancement by gold nanorods for cell surface marker detection
title_sort spr signals enhancement by gold nanorods for cell surface marker detection
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637213/
https://www.ncbi.nlm.nih.gov/pubmed/31334038
http://dx.doi.org/10.15171/bi.2019.10
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