Cargando…

Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients

BACKGROUND: Nosocomial infections and persistence of multidrug resistant biofilm forming Acinetobacter baumannii in hospitals has made it as a serious problem in healthcare settings worldwide. METHODS: A total of 100 A. baumannii clinical isolates from immunocompromised patients hospitalized in ICU...

Descripción completa

Detalles Bibliográficos
Autores principales: Zeighami, Habib, Valadkhani, Fatemeh, Shapouri, Reza, Samadi, Elham, Haghi, Fakhri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637494/
https://www.ncbi.nlm.nih.gov/pubmed/31315572
http://dx.doi.org/10.1186/s12879-019-4272-0
_version_ 1783436250584186880
author Zeighami, Habib
Valadkhani, Fatemeh
Shapouri, Reza
Samadi, Elham
Haghi, Fakhri
author_facet Zeighami, Habib
Valadkhani, Fatemeh
Shapouri, Reza
Samadi, Elham
Haghi, Fakhri
author_sort Zeighami, Habib
collection PubMed
description BACKGROUND: Nosocomial infections and persistence of multidrug resistant biofilm forming Acinetobacter baumannii in hospitals has made it as a serious problem in healthcare settings worldwide. METHODS: A total of 100 A. baumannii clinical isolates from immunocompromised patients hospitalized in ICU were investigated for biofilm formation, the presence of biofilm related genes (bap, ompA, csuE, fimH, epsA, bla(PER-1), bfmS, ptk, pgaB, csgA, kpsMII), integron characterization and molecular typing based on REP-PCR. RESULTS: All isolates were resistant to three or more categories of antibiotics and considered as multidrug resistant (MDR). A total of 32 isolates were resistant to all tested antibiotics and 91% were extensively drug-resistance (XDR). All isolates were able to produce biofilm and 58% of isolates showed strong ability to biofilm formation. All strong biofilm forming A. baumannii isolates were XDR. All A. baumannii isolates carried at least one biofilm related gene. The most prevalent gene was csuE (100%), followed by pgaB (98%), epsA and ptk (95%), bfmS (92%) and ompA (81%). 98% of isolates carried more than 4 biofilm related genes, simultaneously. Class I integron (67%) was more frequent in comparison with class II (10%) (P < 0.05). The REP-PCR patterns were classified as 8 types (A-H) and 21 subtypes. The A1 (23%) and C1 (15%) clusters were the most prevalent among A. baumannii isolates (P < 0.05). According to the REP-PCR patterns, 23% of all isolates had a clonal relatedness. CONCLUSION: Our study revealed the high frequency of biofilm forming XDR A. baumannii in ICU patients, with a high prevalence of biofilm related genes of csuE and pgaB. It seems that the appropriate surveillance and control measures are essential to prevent the emergence and transmission of XDR A. baumannii in our country.
format Online
Article
Text
id pubmed-6637494
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-66374942019-07-25 Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients Zeighami, Habib Valadkhani, Fatemeh Shapouri, Reza Samadi, Elham Haghi, Fakhri BMC Infect Dis Research Article BACKGROUND: Nosocomial infections and persistence of multidrug resistant biofilm forming Acinetobacter baumannii in hospitals has made it as a serious problem in healthcare settings worldwide. METHODS: A total of 100 A. baumannii clinical isolates from immunocompromised patients hospitalized in ICU were investigated for biofilm formation, the presence of biofilm related genes (bap, ompA, csuE, fimH, epsA, bla(PER-1), bfmS, ptk, pgaB, csgA, kpsMII), integron characterization and molecular typing based on REP-PCR. RESULTS: All isolates were resistant to three or more categories of antibiotics and considered as multidrug resistant (MDR). A total of 32 isolates were resistant to all tested antibiotics and 91% were extensively drug-resistance (XDR). All isolates were able to produce biofilm and 58% of isolates showed strong ability to biofilm formation. All strong biofilm forming A. baumannii isolates were XDR. All A. baumannii isolates carried at least one biofilm related gene. The most prevalent gene was csuE (100%), followed by pgaB (98%), epsA and ptk (95%), bfmS (92%) and ompA (81%). 98% of isolates carried more than 4 biofilm related genes, simultaneously. Class I integron (67%) was more frequent in comparison with class II (10%) (P < 0.05). The REP-PCR patterns were classified as 8 types (A-H) and 21 subtypes. The A1 (23%) and C1 (15%) clusters were the most prevalent among A. baumannii isolates (P < 0.05). According to the REP-PCR patterns, 23% of all isolates had a clonal relatedness. CONCLUSION: Our study revealed the high frequency of biofilm forming XDR A. baumannii in ICU patients, with a high prevalence of biofilm related genes of csuE and pgaB. It seems that the appropriate surveillance and control measures are essential to prevent the emergence and transmission of XDR A. baumannii in our country. BioMed Central 2019-07-17 /pmc/articles/PMC6637494/ /pubmed/31315572 http://dx.doi.org/10.1186/s12879-019-4272-0 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zeighami, Habib
Valadkhani, Fatemeh
Shapouri, Reza
Samadi, Elham
Haghi, Fakhri
Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients
title Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients
title_full Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients
title_fullStr Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients
title_full_unstemmed Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients
title_short Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients
title_sort virulence characteristics of multidrug resistant biofilm forming acinetobacter baumannii isolated from intensive care unit patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637494/
https://www.ncbi.nlm.nih.gov/pubmed/31315572
http://dx.doi.org/10.1186/s12879-019-4272-0
work_keys_str_mv AT zeighamihabib virulencecharacteristicsofmultidrugresistantbiofilmformingacinetobacterbaumanniiisolatedfromintensivecareunitpatients
AT valadkhanifatemeh virulencecharacteristicsofmultidrugresistantbiofilmformingacinetobacterbaumanniiisolatedfromintensivecareunitpatients
AT shapourireza virulencecharacteristicsofmultidrugresistantbiofilmformingacinetobacterbaumanniiisolatedfromintensivecareunitpatients
AT samadielham virulencecharacteristicsofmultidrugresistantbiofilmformingacinetobacterbaumanniiisolatedfromintensivecareunitpatients
AT haghifakhri virulencecharacteristicsofmultidrugresistantbiofilmformingacinetobacterbaumanniiisolatedfromintensivecareunitpatients