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Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia

BACKGROUND: Anthrax is a zoonotic disease caused by the Gram-positive bacterium Bacillus anthracis. In Russia, there are more than 35 thousand anthrax stationary unfavourable sites. At the same time, there is very little published information about the isolates of B. anthracis from the territory of...

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Autores principales: Pisarenko, Sergey V., Eremenko, Eugene I., Ryazanova, Alla G., Kovalev, Dmitry A., Buravtseva, Nina P., Aksenova, Lyudmila Yu., Evchenko, Anna Yu., Semenova, Olga V., Bobrisheva, Olga V., Kuznetsova, Irina V., Golovinskaya, Tatyana M., Tchmerenko, Dmitriy K., Kulichenko, Alexander N., Morozov, Vitaliy Yu.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637652/
https://www.ncbi.nlm.nih.gov/pubmed/31315564
http://dx.doi.org/10.1186/s12866-019-1542-3
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author Pisarenko, Sergey V.
Eremenko, Eugene I.
Ryazanova, Alla G.
Kovalev, Dmitry A.
Buravtseva, Nina P.
Aksenova, Lyudmila Yu.
Evchenko, Anna Yu.
Semenova, Olga V.
Bobrisheva, Olga V.
Kuznetsova, Irina V.
Golovinskaya, Tatyana M.
Tchmerenko, Dmitriy K.
Kulichenko, Alexander N.
Morozov, Vitaliy Yu.
author_facet Pisarenko, Sergey V.
Eremenko, Eugene I.
Ryazanova, Alla G.
Kovalev, Dmitry A.
Buravtseva, Nina P.
Aksenova, Lyudmila Yu.
Evchenko, Anna Yu.
Semenova, Olga V.
Bobrisheva, Olga V.
Kuznetsova, Irina V.
Golovinskaya, Tatyana M.
Tchmerenko, Dmitriy K.
Kulichenko, Alexander N.
Morozov, Vitaliy Yu.
author_sort Pisarenko, Sergey V.
collection PubMed
description BACKGROUND: Anthrax is a zoonotic disease caused by the Gram-positive bacterium Bacillus anthracis. In Russia, there are more than 35 thousand anthrax stationary unfavourable sites. At the same time, there is very little published information about the isolates of B. anthracis from the territory of Russia. In this study, we report the use of whole genome sequencing (WGS) and bioinformatics analysis to characterize B. anthracis 81/1 strain isolated in Russia in 1969 from a person during an outbreak of the disease in the Stavropol region. RESULTS: We used 232 B. anthracis genomes, which are currently available in the GenBank database, to determine the place of the Russian isolate in the global phylogeny of B. anthracis. The studied strain was characterized by PCR-based genetic methods, such as Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA), canonical single nucleotide polymorphisms (canSNP), as well as the method of full-genomic analysis of nucleotide polymorphisms (wgSNP). The results indicate that the Russian B. anthracis 81/1 strain belongs to Trans-Eurasion (TEA) group, the most representative in the world. CONCLUSIONS: In this study, the full genomic sequence of virulent B. anthracis strain from Russia was characterized for the first time. As a result of complex phylogenetic analysis, the place of this isolate was determined in the global phylogenetic structure of the B. anthracis population, expanding our knowledge of anthrax phylogeography in Russia. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12866-019-1542-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-66376522019-07-29 Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia Pisarenko, Sergey V. Eremenko, Eugene I. Ryazanova, Alla G. Kovalev, Dmitry A. Buravtseva, Nina P. Aksenova, Lyudmila Yu. Evchenko, Anna Yu. Semenova, Olga V. Bobrisheva, Olga V. Kuznetsova, Irina V. Golovinskaya, Tatyana M. Tchmerenko, Dmitriy K. Kulichenko, Alexander N. Morozov, Vitaliy Yu. BMC Microbiol Research Article BACKGROUND: Anthrax is a zoonotic disease caused by the Gram-positive bacterium Bacillus anthracis. In Russia, there are more than 35 thousand anthrax stationary unfavourable sites. At the same time, there is very little published information about the isolates of B. anthracis from the territory of Russia. In this study, we report the use of whole genome sequencing (WGS) and bioinformatics analysis to characterize B. anthracis 81/1 strain isolated in Russia in 1969 from a person during an outbreak of the disease in the Stavropol region. RESULTS: We used 232 B. anthracis genomes, which are currently available in the GenBank database, to determine the place of the Russian isolate in the global phylogeny of B. anthracis. The studied strain was characterized by PCR-based genetic methods, such as Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA), canonical single nucleotide polymorphisms (canSNP), as well as the method of full-genomic analysis of nucleotide polymorphisms (wgSNP). The results indicate that the Russian B. anthracis 81/1 strain belongs to Trans-Eurasion (TEA) group, the most representative in the world. CONCLUSIONS: In this study, the full genomic sequence of virulent B. anthracis strain from Russia was characterized for the first time. As a result of complex phylogenetic analysis, the place of this isolate was determined in the global phylogenetic structure of the B. anthracis population, expanding our knowledge of anthrax phylogeography in Russia. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12866-019-1542-3) contains supplementary material, which is available to authorized users. BioMed Central 2019-07-17 /pmc/articles/PMC6637652/ /pubmed/31315564 http://dx.doi.org/10.1186/s12866-019-1542-3 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Pisarenko, Sergey V.
Eremenko, Eugene I.
Ryazanova, Alla G.
Kovalev, Dmitry A.
Buravtseva, Nina P.
Aksenova, Lyudmila Yu.
Evchenko, Anna Yu.
Semenova, Olga V.
Bobrisheva, Olga V.
Kuznetsova, Irina V.
Golovinskaya, Tatyana M.
Tchmerenko, Dmitriy K.
Kulichenko, Alexander N.
Morozov, Vitaliy Yu.
Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia
title Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia
title_full Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia
title_fullStr Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia
title_full_unstemmed Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia
title_short Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia
title_sort genotyping and phylogenetic location of one clinical isolate of bacillus anthracis isolated from a human in russia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637652/
https://www.ncbi.nlm.nih.gov/pubmed/31315564
http://dx.doi.org/10.1186/s12866-019-1542-3
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