Comparison of the Antibacterial Efficacy of Commiphora molmol and Sodium Hypochlorite as Root Canal Irrigants against Enterococcus faecalis and Fusobacterium nucleatum

OBJECTIVE: The investigation aims to compare antimicrobial efficacy of the extract of Commiphora molmol, against Enterococcus faecalis and Fusobacterium nucleatum, with sodium hypochlorite (NaOCl). DESIGN: T he dehydrated oleo-gum resin of Commiphora molmol was extracted by using 70% ethanol and was suspended in 99.8% dimethyl sulfoxide (DMSO) as a dissolving agent in a 1:2 volume to produce an aqueous solution at room temperature. Agar-well diffusion and broth microdilution methods assay were utilized to determine both the antimicrobial activity and minimum inhibitory concentration, of alcoholic extract of Commiphora molmol against E. faecalis and F. nucleatum. The values of the inhibition zones were determined based on the concentration of the investigated material. One hundred and forty extracted human premolar teeth were instrumented and immersed in bacterial suspension of E. faecalis or F. nucleatum (70 teeth in each species suspension). Prepared teeth were then immersed in the myrrh extract solution, 2.5% NaOCl, DMSO, or Cefotaxime and incubated for 30 and 60 minutes. RESULTS: The largest inhibition zone diameter for both bacterial species was obtained by the 100mg/100μL concentration. The minimum inhibitory concentration (MIC) was 0.03mg/300μL for both E. faecalis and F. nucleatum. The minimum bactericidal concentration (MBC) results showed that 0.03mg/μL myrrh extract and 2.5% sodium hypochlorite significantly reduced bacterial growth at both 30 and 60 minutes of different treatments of root canals, compared to DMSO group (negative control) and the antibiotic group (positive group). CONCLUSION: Myrrh extract was proven to have considerable antibacterial activity against both F. nucleatum and E. faecalis.