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TALE‐carrying bacterial pathogens trap host nuclear import receptors for facilitation of infection of rice

Many plant‐pathogenic Xanthomonas rely on the secretion of virulence transcription activator‐like effector (TALE) proteins into plant cells to activate plant susceptibility genes to cause disease. The process is dependent on the binding of TALEs to specific elements of host target gene promoters in...

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Autores principales: Hui, Shugang, Shi, Yarui, Tian, Jingjing, Wang, Li, Li, Yueyue, Wang, Shiping, Yuan, Meng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637887/
https://www.ncbi.nlm.nih.gov/pubmed/30499169
http://dx.doi.org/10.1111/mpp.12772
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author Hui, Shugang
Shi, Yarui
Tian, Jingjing
Wang, Li
Li, Yueyue
Wang, Shiping
Yuan, Meng
author_facet Hui, Shugang
Shi, Yarui
Tian, Jingjing
Wang, Li
Li, Yueyue
Wang, Shiping
Yuan, Meng
author_sort Hui, Shugang
collection PubMed
description Many plant‐pathogenic Xanthomonas rely on the secretion of virulence transcription activator‐like effector (TALE) proteins into plant cells to activate plant susceptibility genes to cause disease. The process is dependent on the binding of TALEs to specific elements of host target gene promoters in the plant nucleus. However, it is unclear how TALEs, after injection into host cells, are transferred from the plant cytoplasm into the plant nucleus, which is the key step of successful pathogen infection. Here, we show that the host plant cytoplasm/nuclear shuttle proteins OsImpα1a and OsImpα1b are key components for infection by the TALE‐carrying bacterial pathogens Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), the causal agents of bacterial leaf blight and bacterial leaf streak, respectively, in rice. Direct interaction between the second nuclear localization signal of TALEs of Xoo or Xoc and OsImpα1a or OsImpα1b is required for the transportation of TALEs into the nucleus. Conversely, suppression of the expression of OsImpα1a and OsImpα1b genes attenuates the shuttling of TALEs from the cytoplasm into the nucleus and the induction of susceptibility genes, thus improving the broad‐spectrum disease resistance of rice to Xoo and Xoc. These results provide an applicable strategy for the improvement of resistance to TALE‐carrying pathogens in rice by moderate suppression of the expression of plant nuclear import receptor proteins.
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spelling pubmed-66378872019-09-16 TALE‐carrying bacterial pathogens trap host nuclear import receptors for facilitation of infection of rice Hui, Shugang Shi, Yarui Tian, Jingjing Wang, Li Li, Yueyue Wang, Shiping Yuan, Meng Mol Plant Pathol Original Articles Many plant‐pathogenic Xanthomonas rely on the secretion of virulence transcription activator‐like effector (TALE) proteins into plant cells to activate plant susceptibility genes to cause disease. The process is dependent on the binding of TALEs to specific elements of host target gene promoters in the plant nucleus. However, it is unclear how TALEs, after injection into host cells, are transferred from the plant cytoplasm into the plant nucleus, which is the key step of successful pathogen infection. Here, we show that the host plant cytoplasm/nuclear shuttle proteins OsImpα1a and OsImpα1b are key components for infection by the TALE‐carrying bacterial pathogens Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), the causal agents of bacterial leaf blight and bacterial leaf streak, respectively, in rice. Direct interaction between the second nuclear localization signal of TALEs of Xoo or Xoc and OsImpα1a or OsImpα1b is required for the transportation of TALEs into the nucleus. Conversely, suppression of the expression of OsImpα1a and OsImpα1b genes attenuates the shuttling of TALEs from the cytoplasm into the nucleus and the induction of susceptibility genes, thus improving the broad‐spectrum disease resistance of rice to Xoo and Xoc. These results provide an applicable strategy for the improvement of resistance to TALE‐carrying pathogens in rice by moderate suppression of the expression of plant nuclear import receptor proteins. John Wiley and Sons Inc. 2019-01-09 /pmc/articles/PMC6637887/ /pubmed/30499169 http://dx.doi.org/10.1111/mpp.12772 Text en © 2018 The Authors. Molecular Plant Pathology Published by BSPP and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Hui, Shugang
Shi, Yarui
Tian, Jingjing
Wang, Li
Li, Yueyue
Wang, Shiping
Yuan, Meng
TALE‐carrying bacterial pathogens trap host nuclear import receptors for facilitation of infection of rice
title TALE‐carrying bacterial pathogens trap host nuclear import receptors for facilitation of infection of rice
title_full TALE‐carrying bacterial pathogens trap host nuclear import receptors for facilitation of infection of rice
title_fullStr TALE‐carrying bacterial pathogens trap host nuclear import receptors for facilitation of infection of rice
title_full_unstemmed TALE‐carrying bacterial pathogens trap host nuclear import receptors for facilitation of infection of rice
title_short TALE‐carrying bacterial pathogens trap host nuclear import receptors for facilitation of infection of rice
title_sort tale‐carrying bacterial pathogens trap host nuclear import receptors for facilitation of infection of rice
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637887/
https://www.ncbi.nlm.nih.gov/pubmed/30499169
http://dx.doi.org/10.1111/mpp.12772
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