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A functionally conserved Zn(2)Cys(6) binuclear cluster transcription factor class regulates necrotrophic effector gene expression and host‐specific virulence of two major Pleosporales fungal pathogens of wheat

The fungus Parastagonospora nodorum is the causal agent of Septoria nodorum blotch of wheat (Triticum aestivum). The interaction is mediated by multiple fungal necrotrophic effector–dominant host sensitivity gene interactions. The three best‐characterized effector–sensitivity gene systems are SnToxA...

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Autores principales: Rybak, Kasia, See, Pao Theen, Phan, Huyen T. T., Syme, Robert A., Moffat, Caroline S., Oliver, Richard P., Tan, Kar‐Chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6638278/
https://www.ncbi.nlm.nih.gov/pubmed/27860150
http://dx.doi.org/10.1111/mpp.12511
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author Rybak, Kasia
See, Pao Theen
Phan, Huyen T. T.
Syme, Robert A.
Moffat, Caroline S.
Oliver, Richard P.
Tan, Kar‐Chun
author_facet Rybak, Kasia
See, Pao Theen
Phan, Huyen T. T.
Syme, Robert A.
Moffat, Caroline S.
Oliver, Richard P.
Tan, Kar‐Chun
author_sort Rybak, Kasia
collection PubMed
description The fungus Parastagonospora nodorum is the causal agent of Septoria nodorum blotch of wheat (Triticum aestivum). The interaction is mediated by multiple fungal necrotrophic effector–dominant host sensitivity gene interactions. The three best‐characterized effector–sensitivity gene systems are SnToxA–Tsn1, SnTox1–Snn1 and SnTox3–Snn3. These effector genes are highly expressed during early infection, but expression decreases as the infection progresses to tissue necrosis and sporulation. However, the mechanism of regulation is unknown. We have identified and functionally characterized a gene, referred to as PnPf2, which encodes a putative zinc finger transcription factor. PnPf2 deletion resulted in the down‐regulation of SnToxA and SnTox3 expression. Virulence on Tsn1 and Snn3 wheat cultivars was strongly reduced. The SnTox1–Snn1 interaction remained unaffected. Furthermore, we have also identified and deleted an orthologous PtrPf2 from the tan spot fungus Pyrenophora tritici‐repentis which possesses a near‐identical ToxA that was acquired from P. nodorum via horizontal gene transfer. PtrPf2 deletion also resulted in the down‐regulation of PtrToxA expression and a near‐complete loss of virulence on Tsn1 wheat. We have demonstrated, for the first time, evidence for a functionally conserved signalling component that plays a role in the regulation of a common/horizontally transferred effector found in two major fungal pathogens of wheat.
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spelling pubmed-66382782019-09-16 A functionally conserved Zn(2)Cys(6) binuclear cluster transcription factor class regulates necrotrophic effector gene expression and host‐specific virulence of two major Pleosporales fungal pathogens of wheat Rybak, Kasia See, Pao Theen Phan, Huyen T. T. Syme, Robert A. Moffat, Caroline S. Oliver, Richard P. Tan, Kar‐Chun Mol Plant Pathol Original Articles The fungus Parastagonospora nodorum is the causal agent of Septoria nodorum blotch of wheat (Triticum aestivum). The interaction is mediated by multiple fungal necrotrophic effector–dominant host sensitivity gene interactions. The three best‐characterized effector–sensitivity gene systems are SnToxA–Tsn1, SnTox1–Snn1 and SnTox3–Snn3. These effector genes are highly expressed during early infection, but expression decreases as the infection progresses to tissue necrosis and sporulation. However, the mechanism of regulation is unknown. We have identified and functionally characterized a gene, referred to as PnPf2, which encodes a putative zinc finger transcription factor. PnPf2 deletion resulted in the down‐regulation of SnToxA and SnTox3 expression. Virulence on Tsn1 and Snn3 wheat cultivars was strongly reduced. The SnTox1–Snn1 interaction remained unaffected. Furthermore, we have also identified and deleted an orthologous PtrPf2 from the tan spot fungus Pyrenophora tritici‐repentis which possesses a near‐identical ToxA that was acquired from P. nodorum via horizontal gene transfer. PtrPf2 deletion also resulted in the down‐regulation of PtrToxA expression and a near‐complete loss of virulence on Tsn1 wheat. We have demonstrated, for the first time, evidence for a functionally conserved signalling component that plays a role in the regulation of a common/horizontally transferred effector found in two major fungal pathogens of wheat. John Wiley and Sons Inc. 2017-01-24 /pmc/articles/PMC6638278/ /pubmed/27860150 http://dx.doi.org/10.1111/mpp.12511 Text en © 2016 THE AUTHORS. MOLECULAR PLANT PATHOLOGY PUBLISHED BY BRITISH SOCIETY FOR PLANT PATHOLOGY AND JOHN WILEY & SONS LTD This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Rybak, Kasia
See, Pao Theen
Phan, Huyen T. T.
Syme, Robert A.
Moffat, Caroline S.
Oliver, Richard P.
Tan, Kar‐Chun
A functionally conserved Zn(2)Cys(6) binuclear cluster transcription factor class regulates necrotrophic effector gene expression and host‐specific virulence of two major Pleosporales fungal pathogens of wheat
title A functionally conserved Zn(2)Cys(6) binuclear cluster transcription factor class regulates necrotrophic effector gene expression and host‐specific virulence of two major Pleosporales fungal pathogens of wheat
title_full A functionally conserved Zn(2)Cys(6) binuclear cluster transcription factor class regulates necrotrophic effector gene expression and host‐specific virulence of two major Pleosporales fungal pathogens of wheat
title_fullStr A functionally conserved Zn(2)Cys(6) binuclear cluster transcription factor class regulates necrotrophic effector gene expression and host‐specific virulence of two major Pleosporales fungal pathogens of wheat
title_full_unstemmed A functionally conserved Zn(2)Cys(6) binuclear cluster transcription factor class regulates necrotrophic effector gene expression and host‐specific virulence of two major Pleosporales fungal pathogens of wheat
title_short A functionally conserved Zn(2)Cys(6) binuclear cluster transcription factor class regulates necrotrophic effector gene expression and host‐specific virulence of two major Pleosporales fungal pathogens of wheat
title_sort functionally conserved zn(2)cys(6) binuclear cluster transcription factor class regulates necrotrophic effector gene expression and host‐specific virulence of two major pleosporales fungal pathogens of wheat
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6638278/
https://www.ncbi.nlm.nih.gov/pubmed/27860150
http://dx.doi.org/10.1111/mpp.12511
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