Cargando…

Igf1R/InsR function is required for axon extension and corpus callosum formation

One of the earliest steps during the development of the nervous system is the establishment of neuronal polarity and the formation of an axon. The intrinsic mechanisms that promote axon formation have been extensively analyzed. However, much less is known about the extrinsic signals that initiate ax...

Descripción completa

Detalles Bibliográficos
Autores principales: Jin, Jing, Ravindran, Priyadarshini, Di Meo, Danila, Püschel, Andreas W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6638864/
https://www.ncbi.nlm.nih.gov/pubmed/31318893
http://dx.doi.org/10.1371/journal.pone.0219362
_version_ 1783436372930985984
author Jin, Jing
Ravindran, Priyadarshini
Di Meo, Danila
Püschel, Andreas W.
author_facet Jin, Jing
Ravindran, Priyadarshini
Di Meo, Danila
Püschel, Andreas W.
author_sort Jin, Jing
collection PubMed
description One of the earliest steps during the development of the nervous system is the establishment of neuronal polarity and the formation of an axon. The intrinsic mechanisms that promote axon formation have been extensively analyzed. However, much less is known about the extrinsic signals that initiate axon formation. One of the candidates for these signals is Insulin-like growth factor 1 (Igf1) that acts through the Igf1 (Igf1R) and insulin receptors (InsR). Since Igf1R and InsR may act redundantly we analyzed conditional cortex-specific knockout mice that are deficient for both Igf1r and Insr to determine if they regulate the development of the cortex and the formation of axons in vivo. Our results show that Igf1R/InsR function is required for the normal development of the embryonic hippocampus and cingulate cortex while the lateral cortex does not show apparent defects in the Igf1r;Insr knockout. In the cingulate cortex, the number of intermediate progenitors and deep layer neurons is reduced and the corpus callosum is absent at E17. However, cortical organization and axon formation are not impaired in knockout embryos. In culture, cortical and hippocampal neurons from Igf1r;Insr knockout embryos extend an axon but the length of this axon is severely reduced. Our results indicate that Igf1R/InsR function is required for brain development in a region-specific manner and promotes axon growth but is not essential for neuronal polarization and migration in the developing brain.
format Online
Article
Text
id pubmed-6638864
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-66388642019-07-25 Igf1R/InsR function is required for axon extension and corpus callosum formation Jin, Jing Ravindran, Priyadarshini Di Meo, Danila Püschel, Andreas W. PLoS One Research Article One of the earliest steps during the development of the nervous system is the establishment of neuronal polarity and the formation of an axon. The intrinsic mechanisms that promote axon formation have been extensively analyzed. However, much less is known about the extrinsic signals that initiate axon formation. One of the candidates for these signals is Insulin-like growth factor 1 (Igf1) that acts through the Igf1 (Igf1R) and insulin receptors (InsR). Since Igf1R and InsR may act redundantly we analyzed conditional cortex-specific knockout mice that are deficient for both Igf1r and Insr to determine if they regulate the development of the cortex and the formation of axons in vivo. Our results show that Igf1R/InsR function is required for the normal development of the embryonic hippocampus and cingulate cortex while the lateral cortex does not show apparent defects in the Igf1r;Insr knockout. In the cingulate cortex, the number of intermediate progenitors and deep layer neurons is reduced and the corpus callosum is absent at E17. However, cortical organization and axon formation are not impaired in knockout embryos. In culture, cortical and hippocampal neurons from Igf1r;Insr knockout embryos extend an axon but the length of this axon is severely reduced. Our results indicate that Igf1R/InsR function is required for brain development in a region-specific manner and promotes axon growth but is not essential for neuronal polarization and migration in the developing brain. Public Library of Science 2019-07-18 /pmc/articles/PMC6638864/ /pubmed/31318893 http://dx.doi.org/10.1371/journal.pone.0219362 Text en © 2019 Jin et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jin, Jing
Ravindran, Priyadarshini
Di Meo, Danila
Püschel, Andreas W.
Igf1R/InsR function is required for axon extension and corpus callosum formation
title Igf1R/InsR function is required for axon extension and corpus callosum formation
title_full Igf1R/InsR function is required for axon extension and corpus callosum formation
title_fullStr Igf1R/InsR function is required for axon extension and corpus callosum formation
title_full_unstemmed Igf1R/InsR function is required for axon extension and corpus callosum formation
title_short Igf1R/InsR function is required for axon extension and corpus callosum formation
title_sort igf1r/insr function is required for axon extension and corpus callosum formation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6638864/
https://www.ncbi.nlm.nih.gov/pubmed/31318893
http://dx.doi.org/10.1371/journal.pone.0219362
work_keys_str_mv AT jinjing igf1rinsrfunctionisrequiredforaxonextensionandcorpuscallosumformation
AT ravindranpriyadarshini igf1rinsrfunctionisrequiredforaxonextensionandcorpuscallosumformation
AT dimeodanila igf1rinsrfunctionisrequiredforaxonextensionandcorpuscallosumformation
AT puschelandreasw igf1rinsrfunctionisrequiredforaxonextensionandcorpuscallosumformation