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Testing skin swabbing for DNA sampling in dendrobatid frogs

Skin swabbing, a minimally invasive DNA sampling method recently proposed for adult amphibians, was tested on the dendrobatid frog Allobates femoralis. I compared DNA yield from skin swabs and toe clips by evaluating obtained DNA concentrations and purity of extracts, as well as amplification succes...

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Autor principal: Ringler, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640035/
https://www.ncbi.nlm.nih.gov/pubmed/31327883
http://dx.doi.org/10.1163/15685381-17000206
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author Ringler, Eva
author_facet Ringler, Eva
author_sort Ringler, Eva
collection PubMed
description Skin swabbing, a minimally invasive DNA sampling method recently proposed for adult amphibians, was tested on the dendrobatid frog Allobates femoralis. I compared DNA yield from skin swabs and toe clips by evaluating obtained DNA concentrations and purity of extracts, as well as amplification success using eleven polymorphic microsatellite loci. I also tested whether storing skin swabs for two months at −20°C affected the properties of the extract or microsatellite analysis. Results show that skin swabs of adult A. femoralis suffered from high contamination and yielded significantly lower DNA quality and quantity, resulting in insufficient genotyping success, than DNA obtained from toe clips. The relatively dry skin in dendrobatid frogs may have impeded the collection of sufficient viable cells, and the presence of skin alkaloids and microbiota in the frog mucus may lead to high contamination load of skin swabs.
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spelling pubmed-66400352019-07-19 Testing skin swabbing for DNA sampling in dendrobatid frogs Ringler, Eva Amphib Reptil Article Skin swabbing, a minimally invasive DNA sampling method recently proposed for adult amphibians, was tested on the dendrobatid frog Allobates femoralis. I compared DNA yield from skin swabs and toe clips by evaluating obtained DNA concentrations and purity of extracts, as well as amplification success using eleven polymorphic microsatellite loci. I also tested whether storing skin swabs for two months at −20°C affected the properties of the extract or microsatellite analysis. Results show that skin swabs of adult A. femoralis suffered from high contamination and yielded significantly lower DNA quality and quantity, resulting in insufficient genotyping success, than DNA obtained from toe clips. The relatively dry skin in dendrobatid frogs may have impeded the collection of sufficient viable cells, and the presence of skin alkaloids and microbiota in the frog mucus may lead to high contamination load of skin swabs. 2018-04-24 2018-01 /pmc/articles/PMC6640035/ /pubmed/31327883 http://dx.doi.org/10.1163/15685381-17000206 Text en http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the prevailing CC-BY license at the time of publication. (http://creativecommons.org/licenses/by/4.0)
spellingShingle Article
Ringler, Eva
Testing skin swabbing for DNA sampling in dendrobatid frogs
title Testing skin swabbing for DNA sampling in dendrobatid frogs
title_full Testing skin swabbing for DNA sampling in dendrobatid frogs
title_fullStr Testing skin swabbing for DNA sampling in dendrobatid frogs
title_full_unstemmed Testing skin swabbing for DNA sampling in dendrobatid frogs
title_short Testing skin swabbing for DNA sampling in dendrobatid frogs
title_sort testing skin swabbing for dna sampling in dendrobatid frogs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640035/
https://www.ncbi.nlm.nih.gov/pubmed/31327883
http://dx.doi.org/10.1163/15685381-17000206
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