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Luminescent peptide tagging enables efficient screening for CRISPR-mediated knock-in in human induced pluripotent stem cells

Human pluripotent stem cells are increasingly used for CRISPR-mediated gene targeting in efforts to generate models of human diseases. This is a challenging task because of the high sensitivity of these cells to suboptimal conditions, including CRISPR-associated DNA damage and subsequent rounds of s...

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Detalles Bibliográficos
Autores principales:	Madsen, Ralitsa R., Semple, Robert K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	F1000 Research Limited 2019
Materias:	Research Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640264/ https://www.ncbi.nlm.nih.gov/pubmed/31363496 http://dx.doi.org/10.12688/wellcomeopenres.15119.3

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