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BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei
Due to significant advances in computational biology, protein prediction, together with antigen and epitope design, have rapidly moved from conventional methods, based on experimental approaches, to in silico-based bioinformatics methods. In this context, we report a reverse vaccinology study that i...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640674/ https://www.ncbi.nlm.nih.gov/pubmed/31544878 http://dx.doi.org/10.3390/antib7030026 |
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author | Capelli, Riccardo Peri, Claudio Villa, Riccardo Nithichanon, Arnone Conchillo-Solé, Oscar Yero, Daniel Gagni, Paola Chiari, Marcella Lertmemongkolchai, Ganjana Cretich, Marina Daura, Xavier Bolognesi, Martino Colombo, Giorgio Gourlay, Louise J. |
author_facet | Capelli, Riccardo Peri, Claudio Villa, Riccardo Nithichanon, Arnone Conchillo-Solé, Oscar Yero, Daniel Gagni, Paola Chiari, Marcella Lertmemongkolchai, Ganjana Cretich, Marina Daura, Xavier Bolognesi, Martino Colombo, Giorgio Gourlay, Louise J. |
author_sort | Capelli, Riccardo |
collection | PubMed |
description | Due to significant advances in computational biology, protein prediction, together with antigen and epitope design, have rapidly moved from conventional methods, based on experimental approaches, to in silico-based bioinformatics methods. In this context, we report a reverse vaccinology study that identified a panel of 104 candidate antigens from the Gram-negative bacterial pathogen Burkholderia pseudomallei, which is responsible for the disease melioidosis. B. pseudomallei can cause fatal sepsis in endemic populations in the tropical regions of the world and treatment with antibiotics is mostly ineffective. With the aim of identifying potential vaccine candidates, we report the experimental validation of predicted antigen and type I fimbrial subunit, BPSL1626, which we show is able to recognize and bind human antibodies from the sera of Burkholderia infected patients and to stimulate T-lymphocytes in vitro. The prerequisite for a melioidosis vaccine, in fact, is that both antibody- and cell-mediated immune responses must be triggered. In order to reveal potential antigenic regions of the protein that may aid immunogen re-design, we also report the crystal structure of BPSL1626 at 1.9 Å resolution on which structure-based epitope predictions were based. Overall, our data suggest that BPSL1626 and three epitope regions here-identified can represent viable candidates as potential antigenic molecules. |
format | Online Article Text |
id | pubmed-6640674 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66406742019-09-05 BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei Capelli, Riccardo Peri, Claudio Villa, Riccardo Nithichanon, Arnone Conchillo-Solé, Oscar Yero, Daniel Gagni, Paola Chiari, Marcella Lertmemongkolchai, Ganjana Cretich, Marina Daura, Xavier Bolognesi, Martino Colombo, Giorgio Gourlay, Louise J. Antibodies (Basel) Article Due to significant advances in computational biology, protein prediction, together with antigen and epitope design, have rapidly moved from conventional methods, based on experimental approaches, to in silico-based bioinformatics methods. In this context, we report a reverse vaccinology study that identified a panel of 104 candidate antigens from the Gram-negative bacterial pathogen Burkholderia pseudomallei, which is responsible for the disease melioidosis. B. pseudomallei can cause fatal sepsis in endemic populations in the tropical regions of the world and treatment with antibiotics is mostly ineffective. With the aim of identifying potential vaccine candidates, we report the experimental validation of predicted antigen and type I fimbrial subunit, BPSL1626, which we show is able to recognize and bind human antibodies from the sera of Burkholderia infected patients and to stimulate T-lymphocytes in vitro. The prerequisite for a melioidosis vaccine, in fact, is that both antibody- and cell-mediated immune responses must be triggered. In order to reveal potential antigenic regions of the protein that may aid immunogen re-design, we also report the crystal structure of BPSL1626 at 1.9 Å resolution on which structure-based epitope predictions were based. Overall, our data suggest that BPSL1626 and three epitope regions here-identified can represent viable candidates as potential antigenic molecules. MDPI 2018-07-19 /pmc/articles/PMC6640674/ /pubmed/31544878 http://dx.doi.org/10.3390/antib7030026 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Capelli, Riccardo Peri, Claudio Villa, Riccardo Nithichanon, Arnone Conchillo-Solé, Oscar Yero, Daniel Gagni, Paola Chiari, Marcella Lertmemongkolchai, Ganjana Cretich, Marina Daura, Xavier Bolognesi, Martino Colombo, Giorgio Gourlay, Louise J. BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei |
title | BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei |
title_full | BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei |
title_fullStr | BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei |
title_full_unstemmed | BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei |
title_short | BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei |
title_sort | bpsl1626: reverse and structural vaccinology reveal a novel candidate for vaccine design against burkholderia pseudomallei |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640674/ https://www.ncbi.nlm.nih.gov/pubmed/31544878 http://dx.doi.org/10.3390/antib7030026 |
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