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BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei

Due to significant advances in computational biology, protein prediction, together with antigen and epitope design, have rapidly moved from conventional methods, based on experimental approaches, to in silico-based bioinformatics methods. In this context, we report a reverse vaccinology study that i...

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Autores principales: Capelli, Riccardo, Peri, Claudio, Villa, Riccardo, Nithichanon, Arnone, Conchillo-Solé, Oscar, Yero, Daniel, Gagni, Paola, Chiari, Marcella, Lertmemongkolchai, Ganjana, Cretich, Marina, Daura, Xavier, Bolognesi, Martino, Colombo, Giorgio, Gourlay, Louise J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640674/
https://www.ncbi.nlm.nih.gov/pubmed/31544878
http://dx.doi.org/10.3390/antib7030026
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author Capelli, Riccardo
Peri, Claudio
Villa, Riccardo
Nithichanon, Arnone
Conchillo-Solé, Oscar
Yero, Daniel
Gagni, Paola
Chiari, Marcella
Lertmemongkolchai, Ganjana
Cretich, Marina
Daura, Xavier
Bolognesi, Martino
Colombo, Giorgio
Gourlay, Louise J.
author_facet Capelli, Riccardo
Peri, Claudio
Villa, Riccardo
Nithichanon, Arnone
Conchillo-Solé, Oscar
Yero, Daniel
Gagni, Paola
Chiari, Marcella
Lertmemongkolchai, Ganjana
Cretich, Marina
Daura, Xavier
Bolognesi, Martino
Colombo, Giorgio
Gourlay, Louise J.
author_sort Capelli, Riccardo
collection PubMed
description Due to significant advances in computational biology, protein prediction, together with antigen and epitope design, have rapidly moved from conventional methods, based on experimental approaches, to in silico-based bioinformatics methods. In this context, we report a reverse vaccinology study that identified a panel of 104 candidate antigens from the Gram-negative bacterial pathogen Burkholderia pseudomallei, which is responsible for the disease melioidosis. B. pseudomallei can cause fatal sepsis in endemic populations in the tropical regions of the world and treatment with antibiotics is mostly ineffective. With the aim of identifying potential vaccine candidates, we report the experimental validation of predicted antigen and type I fimbrial subunit, BPSL1626, which we show is able to recognize and bind human antibodies from the sera of Burkholderia infected patients and to stimulate T-lymphocytes in vitro. The prerequisite for a melioidosis vaccine, in fact, is that both antibody- and cell-mediated immune responses must be triggered. In order to reveal potential antigenic regions of the protein that may aid immunogen re-design, we also report the crystal structure of BPSL1626 at 1.9 Å resolution on which structure-based epitope predictions were based. Overall, our data suggest that BPSL1626 and three epitope regions here-identified can represent viable candidates as potential antigenic molecules.
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spelling pubmed-66406742019-09-05 BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei Capelli, Riccardo Peri, Claudio Villa, Riccardo Nithichanon, Arnone Conchillo-Solé, Oscar Yero, Daniel Gagni, Paola Chiari, Marcella Lertmemongkolchai, Ganjana Cretich, Marina Daura, Xavier Bolognesi, Martino Colombo, Giorgio Gourlay, Louise J. Antibodies (Basel) Article Due to significant advances in computational biology, protein prediction, together with antigen and epitope design, have rapidly moved from conventional methods, based on experimental approaches, to in silico-based bioinformatics methods. In this context, we report a reverse vaccinology study that identified a panel of 104 candidate antigens from the Gram-negative bacterial pathogen Burkholderia pseudomallei, which is responsible for the disease melioidosis. B. pseudomallei can cause fatal sepsis in endemic populations in the tropical regions of the world and treatment with antibiotics is mostly ineffective. With the aim of identifying potential vaccine candidates, we report the experimental validation of predicted antigen and type I fimbrial subunit, BPSL1626, which we show is able to recognize and bind human antibodies from the sera of Burkholderia infected patients and to stimulate T-lymphocytes in vitro. The prerequisite for a melioidosis vaccine, in fact, is that both antibody- and cell-mediated immune responses must be triggered. In order to reveal potential antigenic regions of the protein that may aid immunogen re-design, we also report the crystal structure of BPSL1626 at 1.9 Å resolution on which structure-based epitope predictions were based. Overall, our data suggest that BPSL1626 and three epitope regions here-identified can represent viable candidates as potential antigenic molecules. MDPI 2018-07-19 /pmc/articles/PMC6640674/ /pubmed/31544878 http://dx.doi.org/10.3390/antib7030026 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Capelli, Riccardo
Peri, Claudio
Villa, Riccardo
Nithichanon, Arnone
Conchillo-Solé, Oscar
Yero, Daniel
Gagni, Paola
Chiari, Marcella
Lertmemongkolchai, Ganjana
Cretich, Marina
Daura, Xavier
Bolognesi, Martino
Colombo, Giorgio
Gourlay, Louise J.
BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei
title BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei
title_full BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei
title_fullStr BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei
title_full_unstemmed BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei
title_short BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei
title_sort bpsl1626: reverse and structural vaccinology reveal a novel candidate for vaccine design against burkholderia pseudomallei
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640674/
https://www.ncbi.nlm.nih.gov/pubmed/31544878
http://dx.doi.org/10.3390/antib7030026
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