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Proton-irradiated breast cells: molecular points of view
Breast cancer (BC) is the most common cancer in women, highly heterogeneous at both the clinical and molecular level. Radiation therapy (RT) represents an efficient modality to treat localized tumor in BC care, although the choice of a unique treatment plan for all BC patients, including RT, may not...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640903/ https://www.ncbi.nlm.nih.gov/pubmed/31135901 http://dx.doi.org/10.1093/jrr/rrz032 |
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author | Bravatà, Valentina Cammarata, Francesco P Minafra, Luigi Pisciotta, Pietro Scazzone, Concetta Manti, Lorenzo Savoca, Gaetano Petringa, Giada Cirrone, Giuseppe A P Cuttone, Giacomo Gilardi, Maria C Forte, Giusi I Russo, Giorgio |
author_facet | Bravatà, Valentina Cammarata, Francesco P Minafra, Luigi Pisciotta, Pietro Scazzone, Concetta Manti, Lorenzo Savoca, Gaetano Petringa, Giada Cirrone, Giuseppe A P Cuttone, Giacomo Gilardi, Maria C Forte, Giusi I Russo, Giorgio |
author_sort | Bravatà, Valentina |
collection | PubMed |
description | Breast cancer (BC) is the most common cancer in women, highly heterogeneous at both the clinical and molecular level. Radiation therapy (RT) represents an efficient modality to treat localized tumor in BC care, although the choice of a unique treatment plan for all BC patients, including RT, may not be the best option. Technological advances in RT are evolving with the use of charged particle beams (i.e. protons) which, due to a more localized delivery of the radiation dose, reduce the dose administered to the heart compared with conventional RT. However, few data regarding proton-induced molecular changes are currently available. The aim of this study was to investigate and describe the production of immunological molecules and gene expression profiles induced by proton irradiation. We performed Luminex assay and cDNA microarray analyses to study the biological processes activated following irradiation with proton beams, both in the non-tumorigenic MCF10A cell line and in two tumorigenic BC cell lines, MCF7 and MDA-MB-231. The immunological signatures were dose dependent in MCF10A and MCF7 cell lines, whereas MDA-MB-231 cells show a strong pro-inflammatory profile regardless of the dose delivered. Clonogenic assay revealed different surviving fractions according to the breast cell lines analyzed. We found the involvement of genes related to cell response to proton irradiation and reported specific cell line- and dose-dependent gene signatures, able to drive cell fate after radiation exposure. Our data could represent a useful tool to better understand the molecular mechanisms elicited by proton irradiation and to predict treatment outcome |
format | Online Article Text |
id | pubmed-6640903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-66409032019-07-24 Proton-irradiated breast cells: molecular points of view Bravatà, Valentina Cammarata, Francesco P Minafra, Luigi Pisciotta, Pietro Scazzone, Concetta Manti, Lorenzo Savoca, Gaetano Petringa, Giada Cirrone, Giuseppe A P Cuttone, Giacomo Gilardi, Maria C Forte, Giusi I Russo, Giorgio J Radiat Res Regular Paper Breast cancer (BC) is the most common cancer in women, highly heterogeneous at both the clinical and molecular level. Radiation therapy (RT) represents an efficient modality to treat localized tumor in BC care, although the choice of a unique treatment plan for all BC patients, including RT, may not be the best option. Technological advances in RT are evolving with the use of charged particle beams (i.e. protons) which, due to a more localized delivery of the radiation dose, reduce the dose administered to the heart compared with conventional RT. However, few data regarding proton-induced molecular changes are currently available. The aim of this study was to investigate and describe the production of immunological molecules and gene expression profiles induced by proton irradiation. We performed Luminex assay and cDNA microarray analyses to study the biological processes activated following irradiation with proton beams, both in the non-tumorigenic MCF10A cell line and in two tumorigenic BC cell lines, MCF7 and MDA-MB-231. The immunological signatures were dose dependent in MCF10A and MCF7 cell lines, whereas MDA-MB-231 cells show a strong pro-inflammatory profile regardless of the dose delivered. Clonogenic assay revealed different surviving fractions according to the breast cell lines analyzed. We found the involvement of genes related to cell response to proton irradiation and reported specific cell line- and dose-dependent gene signatures, able to drive cell fate after radiation exposure. Our data could represent a useful tool to better understand the molecular mechanisms elicited by proton irradiation and to predict treatment outcome Oxford University Press 2019-07 2019-05-28 /pmc/articles/PMC6640903/ /pubmed/31135901 http://dx.doi.org/10.1093/jrr/rrz032 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Regular Paper Bravatà, Valentina Cammarata, Francesco P Minafra, Luigi Pisciotta, Pietro Scazzone, Concetta Manti, Lorenzo Savoca, Gaetano Petringa, Giada Cirrone, Giuseppe A P Cuttone, Giacomo Gilardi, Maria C Forte, Giusi I Russo, Giorgio Proton-irradiated breast cells: molecular points of view |
title | Proton-irradiated breast cells: molecular points of view |
title_full | Proton-irradiated breast cells: molecular points of view |
title_fullStr | Proton-irradiated breast cells: molecular points of view |
title_full_unstemmed | Proton-irradiated breast cells: molecular points of view |
title_short | Proton-irradiated breast cells: molecular points of view |
title_sort | proton-irradiated breast cells: molecular points of view |
topic | Regular Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640903/ https://www.ncbi.nlm.nih.gov/pubmed/31135901 http://dx.doi.org/10.1093/jrr/rrz032 |
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