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Total body irradiation induced mouse small intestine senescence as a late effect

Radiation can induce senescence in many organs and tissues; however, it is still unclear how radiation stimulates senescence in mouse small intestine. In this study, we use the bone marrow transplantation mouse model to explore the late effects of total body irradiation on small intestine. Our resul...

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Autores principales: Zhao, Yu, Zhang, Junling, Han, Xiaodan, Fan, Saijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641339/
https://www.ncbi.nlm.nih.gov/pubmed/31165161
http://dx.doi.org/10.1093/jrr/rrz026
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author Zhao, Yu
Zhang, Junling
Han, Xiaodan
Fan, Saijun
author_facet Zhao, Yu
Zhang, Junling
Han, Xiaodan
Fan, Saijun
author_sort Zhao, Yu
collection PubMed
description Radiation can induce senescence in many organs and tissues; however, it is still unclear how radiation stimulates senescence in mouse small intestine. In this study, we use the bone marrow transplantation mouse model to explore the late effects of total body irradiation on small intestine. Our results showed that almost all of the body hairs of the irradiated mice were white (which is an indication of aging) 10 months after the exposure to radiation. Furthermore, compared with the age-matched control mice, there were more SA-β-galactosidase (SA-β-gal)–positive cells and an upregulation of p16 and p21 in 8 Gy–irradiated mice intestinal crypts, indicating that radiation induced senescence in the small intestine. Intestinal bacterial flora profile analysis showed that the diversity of the intestinal bacterial flora decreased in irradiated mice; in addition it showed that the principal components of the irradiated and control mice differed: there was increased abundance of Bacteroidia and a decreased abundance of Clostridia in irradiated mice. To explore the underlying mechanism, an RNA-sequence was executed; the results suggested that pancreatic secretion, and the digestion and absorption of proteins, carbohydrates, fats and vitamins were damaged in irradiated mice, which may be responsible for the body weight loss observed in irradiated mice. In summary, our study suggested that total body irradiation may induce senescence in the small intestine and damage the health status of the irradiated mice.
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spelling pubmed-66413392019-07-24 Total body irradiation induced mouse small intestine senescence as a late effect Zhao, Yu Zhang, Junling Han, Xiaodan Fan, Saijun J Radiat Res Regular Paper Radiation can induce senescence in many organs and tissues; however, it is still unclear how radiation stimulates senescence in mouse small intestine. In this study, we use the bone marrow transplantation mouse model to explore the late effects of total body irradiation on small intestine. Our results showed that almost all of the body hairs of the irradiated mice were white (which is an indication of aging) 10 months after the exposure to radiation. Furthermore, compared with the age-matched control mice, there were more SA-β-galactosidase (SA-β-gal)–positive cells and an upregulation of p16 and p21 in 8 Gy–irradiated mice intestinal crypts, indicating that radiation induced senescence in the small intestine. Intestinal bacterial flora profile analysis showed that the diversity of the intestinal bacterial flora decreased in irradiated mice; in addition it showed that the principal components of the irradiated and control mice differed: there was increased abundance of Bacteroidia and a decreased abundance of Clostridia in irradiated mice. To explore the underlying mechanism, an RNA-sequence was executed; the results suggested that pancreatic secretion, and the digestion and absorption of proteins, carbohydrates, fats and vitamins were damaged in irradiated mice, which may be responsible for the body weight loss observed in irradiated mice. In summary, our study suggested that total body irradiation may induce senescence in the small intestine and damage the health status of the irradiated mice. Oxford University Press 2019-07 2019-06-05 /pmc/articles/PMC6641339/ /pubmed/31165161 http://dx.doi.org/10.1093/jrr/rrz026 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Regular Paper
Zhao, Yu
Zhang, Junling
Han, Xiaodan
Fan, Saijun
Total body irradiation induced mouse small intestine senescence as a late effect
title Total body irradiation induced mouse small intestine senescence as a late effect
title_full Total body irradiation induced mouse small intestine senescence as a late effect
title_fullStr Total body irradiation induced mouse small intestine senescence as a late effect
title_full_unstemmed Total body irradiation induced mouse small intestine senescence as a late effect
title_short Total body irradiation induced mouse small intestine senescence as a late effect
title_sort total body irradiation induced mouse small intestine senescence as a late effect
topic Regular Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641339/
https://www.ncbi.nlm.nih.gov/pubmed/31165161
http://dx.doi.org/10.1093/jrr/rrz026
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