Development of Indirect Competitive Enzyme-Linked Immunosorbent and Immunochromatographic Strip Assays for Tiamulin Detection in Chicken

[Image: see text] Tiamulin (TML) is a diterpenoid antibiotic used in animals. In this study, a gold nanoparticle immunochromatographic strip assay and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed to detect the residue of TML in chicken. TML aminobutyric was syn...

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Autores principales: Lei, Xianlu, Song, Shanshan, Tao, Hong, Liu, Liqiang, Zheng, Qiankun, Xu, Chuanlai, Kuang, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641450/
https://www.ncbi.nlm.nih.gov/pubmed/31458609
http://dx.doi.org/10.1021/acsomega.8b00289
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author Lei, Xianlu
Song, Shanshan
Tao, Hong
Liu, Liqiang
Zheng, Qiankun
Xu, Chuanlai
Kuang, Hua
author_facet Lei, Xianlu
Song, Shanshan
Tao, Hong
Liu, Liqiang
Zheng, Qiankun
Xu, Chuanlai
Kuang, Hua
author_sort Lei, Xianlu
collection PubMed
description [Image: see text] Tiamulin (TML) is a diterpenoid antibiotic used in animals. In this study, a gold nanoparticle immunochromatographic strip assay and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed to detect the residue of TML in chicken. TML aminobutyric was synthesized and conjugated to keyhole limpet hemocyanin by mixed anhydride method as immunogen, whereas TML was connected to ovalbumin with 1,1′-carbonyldiimidazole as coating antigen. Under optimized conditions, the ultrasensitive monoclonal antibody-based ic-ELISA exhibited a half-maximal inhibitory concentration (IC(50)) value of 0.36 ng/mL with a working range of 0.14–0.9 ng/mL for TML. A rapid and sensitive immunochromatographic strip assay was developed with a TML cutoff value of 2.5 ng/mL. On the basis of these results, both developed methods are useful for TML detection in chicken.
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spelling pubmed-66414502019-08-27 Development of Indirect Competitive Enzyme-Linked Immunosorbent and Immunochromatographic Strip Assays for Tiamulin Detection in Chicken Lei, Xianlu Song, Shanshan Tao, Hong Liu, Liqiang Zheng, Qiankun Xu, Chuanlai Kuang, Hua ACS Omega [Image: see text] Tiamulin (TML) is a diterpenoid antibiotic used in animals. In this study, a gold nanoparticle immunochromatographic strip assay and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed to detect the residue of TML in chicken. TML aminobutyric was synthesized and conjugated to keyhole limpet hemocyanin by mixed anhydride method as immunogen, whereas TML was connected to ovalbumin with 1,1′-carbonyldiimidazole as coating antigen. Under optimized conditions, the ultrasensitive monoclonal antibody-based ic-ELISA exhibited a half-maximal inhibitory concentration (IC(50)) value of 0.36 ng/mL with a working range of 0.14–0.9 ng/mL for TML. A rapid and sensitive immunochromatographic strip assay was developed with a TML cutoff value of 2.5 ng/mL. On the basis of these results, both developed methods are useful for TML detection in chicken. American Chemical Society 2018-03-28 /pmc/articles/PMC6641450/ /pubmed/31458609 http://dx.doi.org/10.1021/acsomega.8b00289 Text en Copyright © 2018 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Lei, Xianlu
Song, Shanshan
Tao, Hong
Liu, Liqiang
Zheng, Qiankun
Xu, Chuanlai
Kuang, Hua
Development of Indirect Competitive Enzyme-Linked Immunosorbent and Immunochromatographic Strip Assays for Tiamulin Detection in Chicken
title Development of Indirect Competitive Enzyme-Linked Immunosorbent and Immunochromatographic Strip Assays for Tiamulin Detection in Chicken
title_full Development of Indirect Competitive Enzyme-Linked Immunosorbent and Immunochromatographic Strip Assays for Tiamulin Detection in Chicken
title_fullStr Development of Indirect Competitive Enzyme-Linked Immunosorbent and Immunochromatographic Strip Assays for Tiamulin Detection in Chicken
title_full_unstemmed Development of Indirect Competitive Enzyme-Linked Immunosorbent and Immunochromatographic Strip Assays for Tiamulin Detection in Chicken
title_short Development of Indirect Competitive Enzyme-Linked Immunosorbent and Immunochromatographic Strip Assays for Tiamulin Detection in Chicken
title_sort development of indirect competitive enzyme-linked immunosorbent and immunochromatographic strip assays for tiamulin detection in chicken
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641450/
https://www.ncbi.nlm.nih.gov/pubmed/31458609
http://dx.doi.org/10.1021/acsomega.8b00289
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