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Deciphering Design Principles of Förster Resonance Energy Transfer-Based Protease Substrates: Thermolysin-Like Protease from Geobacillus stearothermophilus as a Test Case

[Image: see text] Protease activity is frequently assayed using short peptides that are equipped with a Förster resonance energy transfer (FRET) reporter system. Many frequently used donor–acceptor pairs are excited in the ultraviolet range and suffer from low extinction coefficients and quantum yie...

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Detalles Bibliográficos
Autores principales:	Ripp, Sophie, Turunen, Petri, Minot, Ethan D., Rowan, Alan E., Blank, Kerstin G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2018
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641592/ https://www.ncbi.nlm.nih.gov/pubmed/31458650 http://dx.doi.org/10.1021/acsomega.7b02084

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