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Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics
[Image: see text] Conventional studies on enzyme kinetics assume that the substrate concentration remains constant. However, for catalytic reactions taking place in intracellular compartments, the substrate molecules are fed in and out of the compartment and are catalyzed into product molecules with...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641702/ https://www.ncbi.nlm.nih.gov/pubmed/31458761 http://dx.doi.org/10.1021/acsomega.8b00611 |
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author | Singh, Divya Chaudhury, Srabanti |
author_facet | Singh, Divya Chaudhury, Srabanti |
author_sort | Singh, Divya |
collection | PubMed |
description | [Image: see text] Conventional studies on enzyme kinetics assume that the substrate concentration remains constant. However, for catalytic reactions taking place in intracellular compartments, the substrate molecules are fed in and out of the compartment and are catalyzed into product molecules within the compartment. In this work, we use a chemical master equation approach to study the stochastic kinetics of a single enzyme for different reaction pathways with one or more intermediate states. We obtain velocity expressions that deviate from the Michaelis–Menten expression. We study the coefficient of variation, which is a measure of the noise strength as a function of the mean substrate concentration for systems where there is influx or/and outflux of substrate molecules. Our results show that the noise strength decreases with the increase in the substrate concentration and finally remains the same when the substrate is present in abundance. |
format | Online Article Text |
id | pubmed-6641702 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-66417022019-08-27 Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics Singh, Divya Chaudhury, Srabanti ACS Omega [Image: see text] Conventional studies on enzyme kinetics assume that the substrate concentration remains constant. However, for catalytic reactions taking place in intracellular compartments, the substrate molecules are fed in and out of the compartment and are catalyzed into product molecules within the compartment. In this work, we use a chemical master equation approach to study the stochastic kinetics of a single enzyme for different reaction pathways with one or more intermediate states. We obtain velocity expressions that deviate from the Michaelis–Menten expression. We study the coefficient of variation, which is a measure of the noise strength as a function of the mean substrate concentration for systems where there is influx or/and outflux of substrate molecules. Our results show that the noise strength decreases with the increase in the substrate concentration and finally remains the same when the substrate is present in abundance. American Chemical Society 2018-05-24 /pmc/articles/PMC6641702/ /pubmed/31458761 http://dx.doi.org/10.1021/acsomega.8b00611 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Singh, Divya Chaudhury, Srabanti Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics |
title | Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics |
title_full | Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics |
title_fullStr | Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics |
title_full_unstemmed | Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics |
title_short | Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics |
title_sort | effect of substrate number fluctuations in stochastic enzyme kinetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641702/ https://www.ncbi.nlm.nih.gov/pubmed/31458761 http://dx.doi.org/10.1021/acsomega.8b00611 |
work_keys_str_mv | AT singhdivya effectofsubstratenumberfluctuationsinstochasticenzymekinetics AT chaudhurysrabanti effectofsubstratenumberfluctuationsinstochasticenzymekinetics |