Cargando…

Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics

[Image: see text] Conventional studies on enzyme kinetics assume that the substrate concentration remains constant. However, for catalytic reactions taking place in intracellular compartments, the substrate molecules are fed in and out of the compartment and are catalyzed into product molecules with...

Descripción completa

Detalles Bibliográficos
Autores principales: Singh, Divya, Chaudhury, Srabanti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641702/
https://www.ncbi.nlm.nih.gov/pubmed/31458761
http://dx.doi.org/10.1021/acsomega.8b00611
_version_ 1783436834474295296
author Singh, Divya
Chaudhury, Srabanti
author_facet Singh, Divya
Chaudhury, Srabanti
author_sort Singh, Divya
collection PubMed
description [Image: see text] Conventional studies on enzyme kinetics assume that the substrate concentration remains constant. However, for catalytic reactions taking place in intracellular compartments, the substrate molecules are fed in and out of the compartment and are catalyzed into product molecules within the compartment. In this work, we use a chemical master equation approach to study the stochastic kinetics of a single enzyme for different reaction pathways with one or more intermediate states. We obtain velocity expressions that deviate from the Michaelis–Menten expression. We study the coefficient of variation, which is a measure of the noise strength as a function of the mean substrate concentration for systems where there is influx or/and outflux of substrate molecules. Our results show that the noise strength decreases with the increase in the substrate concentration and finally remains the same when the substrate is present in abundance.
format Online
Article
Text
id pubmed-6641702
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-66417022019-08-27 Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics Singh, Divya Chaudhury, Srabanti ACS Omega [Image: see text] Conventional studies on enzyme kinetics assume that the substrate concentration remains constant. However, for catalytic reactions taking place in intracellular compartments, the substrate molecules are fed in and out of the compartment and are catalyzed into product molecules within the compartment. In this work, we use a chemical master equation approach to study the stochastic kinetics of a single enzyme for different reaction pathways with one or more intermediate states. We obtain velocity expressions that deviate from the Michaelis–Menten expression. We study the coefficient of variation, which is a measure of the noise strength as a function of the mean substrate concentration for systems where there is influx or/and outflux of substrate molecules. Our results show that the noise strength decreases with the increase in the substrate concentration and finally remains the same when the substrate is present in abundance. American Chemical Society 2018-05-24 /pmc/articles/PMC6641702/ /pubmed/31458761 http://dx.doi.org/10.1021/acsomega.8b00611 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Singh, Divya
Chaudhury, Srabanti
Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics
title Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics
title_full Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics
title_fullStr Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics
title_full_unstemmed Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics
title_short Effect of Substrate Number Fluctuations in Stochastic Enzyme Kinetics
title_sort effect of substrate number fluctuations in stochastic enzyme kinetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641702/
https://www.ncbi.nlm.nih.gov/pubmed/31458761
http://dx.doi.org/10.1021/acsomega.8b00611
work_keys_str_mv AT singhdivya effectofsubstratenumberfluctuationsinstochasticenzymekinetics
AT chaudhurysrabanti effectofsubstratenumberfluctuationsinstochasticenzymekinetics