Cargando…

MC4R and HNF4α promoter methylation at birth contribute to triglyceride levels in childhood: A prospective cohort study

Although the changes in DNA methylation are assumed to be due to the association between adverse intrauterine conditions and adult metabolic health, evidence from human studies is rare. Little is known about the changes in DNA methylation present at birth that affect metabolic profiles in childhood....

Descripción completa

Detalles Bibliográficos
Autores principales: Kwon, Eun Jin, Lee, Hye Ah, You, Young-Ah, Yoo, Jae Young, Park, Hyesook, Park, Eun Ae, Ha, Eun Hee, Kim, Young Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641802/
https://www.ncbi.nlm.nih.gov/pubmed/31305461
http://dx.doi.org/10.1097/MD.0000000000016424
_version_ 1783436858166870016
author Kwon, Eun Jin
Lee, Hye Ah
You, Young-Ah
Yoo, Jae Young
Park, Hyesook
Park, Eun Ae
Ha, Eun Hee
Kim, Young Ju
author_facet Kwon, Eun Jin
Lee, Hye Ah
You, Young-Ah
Yoo, Jae Young
Park, Hyesook
Park, Eun Ae
Ha, Eun Hee
Kim, Young Ju
author_sort Kwon, Eun Jin
collection PubMed
description Although the changes in DNA methylation are assumed to be due to the association between adverse intrauterine conditions and adult metabolic health, evidence from human studies is rare. Little is known about the changes in DNA methylation present at birth that affect metabolic profiles in childhood. Previous studies have shown that the melanocortin 4 receptor (MC4R) and hepatocyte nuclear factor 4 alpha (HNF4α) genes are associated with obesity and metabolic disorders. Thus, we investigated the associations of the DNA methylation statuses of MC4R and HNF4α in cord blood with metabolic profiles in childhood. We collected data from 90 children 7 to 9 years of age included in the Ewha Birth & Growth Cohort Study in Korea. DNA methylation was analyzed by pyrosequencing. The children were split into 2 groups according to the cutoff triglyceride (TG) levels (<110 and ≥110 mg/dL). The methylation statuses of MC4R and HNF4α at birth were significantly associated with the TG level in childhood (P < .05). It was interesting to note that the methylation statuses of MC4R and HNF4α in cord blood were significantly decreased, whereas childhood body mass index was significantly increased, in children with high TG levels compared with children with low TG levels (P < .05). Our findings show that the methylation statuses of MC4R and HNF4α at birth are associated with metabolic profiles in childhood. These epigenetic modifications occurring in early life may contribute to subsequent metabolic-related disorders. Thus, we suggest that DNA methylation status in cord blood may be predictive of the risk of developing metabolic syndrome.
format Online
Article
Text
id pubmed-6641802
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Wolters Kluwer Health
record_format MEDLINE/PubMed
spelling pubmed-66418022019-08-15 MC4R and HNF4α promoter methylation at birth contribute to triglyceride levels in childhood: A prospective cohort study Kwon, Eun Jin Lee, Hye Ah You, Young-Ah Yoo, Jae Young Park, Hyesook Park, Eun Ae Ha, Eun Hee Kim, Young Ju Medicine (Baltimore) Research Article Although the changes in DNA methylation are assumed to be due to the association between adverse intrauterine conditions and adult metabolic health, evidence from human studies is rare. Little is known about the changes in DNA methylation present at birth that affect metabolic profiles in childhood. Previous studies have shown that the melanocortin 4 receptor (MC4R) and hepatocyte nuclear factor 4 alpha (HNF4α) genes are associated with obesity and metabolic disorders. Thus, we investigated the associations of the DNA methylation statuses of MC4R and HNF4α in cord blood with metabolic profiles in childhood. We collected data from 90 children 7 to 9 years of age included in the Ewha Birth & Growth Cohort Study in Korea. DNA methylation was analyzed by pyrosequencing. The children were split into 2 groups according to the cutoff triglyceride (TG) levels (<110 and ≥110 mg/dL). The methylation statuses of MC4R and HNF4α at birth were significantly associated with the TG level in childhood (P < .05). It was interesting to note that the methylation statuses of MC4R and HNF4α in cord blood were significantly decreased, whereas childhood body mass index was significantly increased, in children with high TG levels compared with children with low TG levels (P < .05). Our findings show that the methylation statuses of MC4R and HNF4α at birth are associated with metabolic profiles in childhood. These epigenetic modifications occurring in early life may contribute to subsequent metabolic-related disorders. Thus, we suggest that DNA methylation status in cord blood may be predictive of the risk of developing metabolic syndrome. Wolters Kluwer Health 2019-07-12 /pmc/articles/PMC6641802/ /pubmed/31305461 http://dx.doi.org/10.1097/MD.0000000000016424 Text en Copyright © 2019 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0
spellingShingle Research Article
Kwon, Eun Jin
Lee, Hye Ah
You, Young-Ah
Yoo, Jae Young
Park, Hyesook
Park, Eun Ae
Ha, Eun Hee
Kim, Young Ju
MC4R and HNF4α promoter methylation at birth contribute to triglyceride levels in childhood: A prospective cohort study
title MC4R and HNF4α promoter methylation at birth contribute to triglyceride levels in childhood: A prospective cohort study
title_full MC4R and HNF4α promoter methylation at birth contribute to triglyceride levels in childhood: A prospective cohort study
title_fullStr MC4R and HNF4α promoter methylation at birth contribute to triglyceride levels in childhood: A prospective cohort study
title_full_unstemmed MC4R and HNF4α promoter methylation at birth contribute to triglyceride levels in childhood: A prospective cohort study
title_short MC4R and HNF4α promoter methylation at birth contribute to triglyceride levels in childhood: A prospective cohort study
title_sort mc4r and hnf4α promoter methylation at birth contribute to triglyceride levels in childhood: a prospective cohort study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641802/
https://www.ncbi.nlm.nih.gov/pubmed/31305461
http://dx.doi.org/10.1097/MD.0000000000016424
work_keys_str_mv AT kwoneunjin mc4randhnf4apromotermethylationatbirthcontributetotriglyceridelevelsinchildhoodaprospectivecohortstudy
AT leehyeah mc4randhnf4apromotermethylationatbirthcontributetotriglyceridelevelsinchildhoodaprospectivecohortstudy
AT youyoungah mc4randhnf4apromotermethylationatbirthcontributetotriglyceridelevelsinchildhoodaprospectivecohortstudy
AT yoojaeyoung mc4randhnf4apromotermethylationatbirthcontributetotriglyceridelevelsinchildhoodaprospectivecohortstudy
AT parkhyesook mc4randhnf4apromotermethylationatbirthcontributetotriglyceridelevelsinchildhoodaprospectivecohortstudy
AT parkeunae mc4randhnf4apromotermethylationatbirthcontributetotriglyceridelevelsinchildhoodaprospectivecohortstudy
AT haeunhee mc4randhnf4apromotermethylationatbirthcontributetotriglyceridelevelsinchildhoodaprospectivecohortstudy
AT kimyoungju mc4randhnf4apromotermethylationatbirthcontributetotriglyceridelevelsinchildhoodaprospectivecohortstudy