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High BRAF(V600E) mutation frequency in Chinese patients with papillary thyroid carcinoma increases diagnostic efficacy in cytologically indeterminate thyroid nodules
To estimate the BRAFV600E mutation frequency in Chinese patients with papillary thyroid carcinoma (PTC), and the diagnostic value of BRAFV600E mutation status in thyroid nodules with indeterminate TBSRTC categories. A total of 4875 consecutive samples for thyroid ultrasound-guided fine-needle aspira...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641833/ https://www.ncbi.nlm.nih.gov/pubmed/31305422 http://dx.doi.org/10.1097/MD.0000000000016343 |
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author | Li, Xing-Jia Mao, Xiao-dong Chen, Guo-fang Wang, Qi-feng Chu, Xiao-qiu Hu, Xin Ding, Wen-bo Zeng, Zheng Wang, Jian-hua Xu, Shu-hang Liu, Chao |
author_facet | Li, Xing-Jia Mao, Xiao-dong Chen, Guo-fang Wang, Qi-feng Chu, Xiao-qiu Hu, Xin Ding, Wen-bo Zeng, Zheng Wang, Jian-hua Xu, Shu-hang Liu, Chao |
author_sort | Li, Xing-Jia |
collection | PubMed |
description | To estimate the BRAFV600E mutation frequency in Chinese patients with papillary thyroid carcinoma (PTC), and the diagnostic value of BRAFV600E mutation status in thyroid nodules with indeterminate TBSRTC categories. A total of 4875 consecutive samples for thyroid ultrasound-guided fine-needle aspiration cytology (FNAC) and BRAF(V600E) mutation analysis were collected from patients at Jiangsu Province Hospital on Integration of Chinese and Western Medicine. Among all the cases, 314 underwent thyroidectomy. According to TBSRTC categories, FNAC was performed for a preoperative diagnosis. ROC of the subject was constructed to evaluate the diagnostic value of these 2 methods and their combination. BRAF(V600E) mutation in FNAC of thyroid nodules occurred in 2796 samples (57.35%). Of 353 nodule samples from 314 patients with thyroid operation, 333 were pathologically diagnosed as PTC. Of these PTC patients, 292 (87.69%) were found to have BRAF(V600E) mutation in their preoperative FNAC. In 175 cytologically indeterminate thyroid nodules, BRAF(V600E) mutation identified 88% of PTC. According to ROC data, BRAF(V600E) mutation testing had an obviously higher sensitivity (87.69%) and specificity (100.00%) than TBSRTC. Combining BRAF(V600E) mutation testing and TBSRTC achieved the largest AUC (0.954). For 41 PTC with a negative BRAF(V600E) mutation in preoperative evaluation, the repeated BRAF(V600E) mutation testing found out 12 samples with BRAF(V600E) mutation. The true BRAF(V600E) mutation rate of Chinese PTC patients was 91.29%. Chinese patients with PTC have a higher frequency of BRAF(V600E) mutation. The BRAF(V600E) mutation testing affords a high diagnostic value in thyroid nodules with indeterminate cytology. |
format | Online Article Text |
id | pubmed-6641833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Wolters Kluwer Health |
record_format | MEDLINE/PubMed |
spelling | pubmed-66418332019-08-15 High BRAF(V600E) mutation frequency in Chinese patients with papillary thyroid carcinoma increases diagnostic efficacy in cytologically indeterminate thyroid nodules Li, Xing-Jia Mao, Xiao-dong Chen, Guo-fang Wang, Qi-feng Chu, Xiao-qiu Hu, Xin Ding, Wen-bo Zeng, Zheng Wang, Jian-hua Xu, Shu-hang Liu, Chao Medicine (Baltimore) Research Article To estimate the BRAFV600E mutation frequency in Chinese patients with papillary thyroid carcinoma (PTC), and the diagnostic value of BRAFV600E mutation status in thyroid nodules with indeterminate TBSRTC categories. A total of 4875 consecutive samples for thyroid ultrasound-guided fine-needle aspiration cytology (FNAC) and BRAF(V600E) mutation analysis were collected from patients at Jiangsu Province Hospital on Integration of Chinese and Western Medicine. Among all the cases, 314 underwent thyroidectomy. According to TBSRTC categories, FNAC was performed for a preoperative diagnosis. ROC of the subject was constructed to evaluate the diagnostic value of these 2 methods and their combination. BRAF(V600E) mutation in FNAC of thyroid nodules occurred in 2796 samples (57.35%). Of 353 nodule samples from 314 patients with thyroid operation, 333 were pathologically diagnosed as PTC. Of these PTC patients, 292 (87.69%) were found to have BRAF(V600E) mutation in their preoperative FNAC. In 175 cytologically indeterminate thyroid nodules, BRAF(V600E) mutation identified 88% of PTC. According to ROC data, BRAF(V600E) mutation testing had an obviously higher sensitivity (87.69%) and specificity (100.00%) than TBSRTC. Combining BRAF(V600E) mutation testing and TBSRTC achieved the largest AUC (0.954). For 41 PTC with a negative BRAF(V600E) mutation in preoperative evaluation, the repeated BRAF(V600E) mutation testing found out 12 samples with BRAF(V600E) mutation. The true BRAF(V600E) mutation rate of Chinese PTC patients was 91.29%. Chinese patients with PTC have a higher frequency of BRAF(V600E) mutation. The BRAF(V600E) mutation testing affords a high diagnostic value in thyroid nodules with indeterminate cytology. Wolters Kluwer Health 2019-07-12 /pmc/articles/PMC6641833/ /pubmed/31305422 http://dx.doi.org/10.1097/MD.0000000000016343 Text en Copyright © 2019 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0 |
spellingShingle | Research Article Li, Xing-Jia Mao, Xiao-dong Chen, Guo-fang Wang, Qi-feng Chu, Xiao-qiu Hu, Xin Ding, Wen-bo Zeng, Zheng Wang, Jian-hua Xu, Shu-hang Liu, Chao High BRAF(V600E) mutation frequency in Chinese patients with papillary thyroid carcinoma increases diagnostic efficacy in cytologically indeterminate thyroid nodules |
title | High BRAF(V600E) mutation frequency in Chinese patients with papillary thyroid carcinoma increases diagnostic efficacy in cytologically indeterminate thyroid nodules |
title_full | High BRAF(V600E) mutation frequency in Chinese patients with papillary thyroid carcinoma increases diagnostic efficacy in cytologically indeterminate thyroid nodules |
title_fullStr | High BRAF(V600E) mutation frequency in Chinese patients with papillary thyroid carcinoma increases diagnostic efficacy in cytologically indeterminate thyroid nodules |
title_full_unstemmed | High BRAF(V600E) mutation frequency in Chinese patients with papillary thyroid carcinoma increases diagnostic efficacy in cytologically indeterminate thyroid nodules |
title_short | High BRAF(V600E) mutation frequency in Chinese patients with papillary thyroid carcinoma increases diagnostic efficacy in cytologically indeterminate thyroid nodules |
title_sort | high braf(v600e) mutation frequency in chinese patients with papillary thyroid carcinoma increases diagnostic efficacy in cytologically indeterminate thyroid nodules |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641833/ https://www.ncbi.nlm.nih.gov/pubmed/31305422 http://dx.doi.org/10.1097/MD.0000000000016343 |
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