Cargando…

Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila

Olfactory associative learning in Drosophila is mediated by synaptic plasticity between the Kenyon cells of the mushroom body and their output neurons. Both Kenyon cells and their inputs from projection neurons are cholinergic, yet little is known about the physiological function of muscarinic acety...

Descripción completa

Detalles Bibliográficos
Autores principales: Bielopolski, Noa, Amin, Hoger, Apostolopoulou, Anthi A, Rozenfeld, Eyal, Lerner, Hadas, Huetteroth, Wolf, Lin, Andrew C, Parnas, Moshe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641838/
https://www.ncbi.nlm.nih.gov/pubmed/31215865
http://dx.doi.org/10.7554/eLife.48264
_version_ 1783436866834399232
author Bielopolski, Noa
Amin, Hoger
Apostolopoulou, Anthi A
Rozenfeld, Eyal
Lerner, Hadas
Huetteroth, Wolf
Lin, Andrew C
Parnas, Moshe
author_facet Bielopolski, Noa
Amin, Hoger
Apostolopoulou, Anthi A
Rozenfeld, Eyal
Lerner, Hadas
Huetteroth, Wolf
Lin, Andrew C
Parnas, Moshe
author_sort Bielopolski, Noa
collection PubMed
description Olfactory associative learning in Drosophila is mediated by synaptic plasticity between the Kenyon cells of the mushroom body and their output neurons. Both Kenyon cells and their inputs from projection neurons are cholinergic, yet little is known about the physiological function of muscarinic acetylcholine receptors in learning in adult flies. Here, we show that aversive olfactory learning in adult flies requires type A muscarinic acetylcholine receptors (mAChR-A), particularly in the gamma subtype of Kenyon cells. mAChR-A inhibits odor responses and is localized in Kenyon cell dendrites. Moreover, mAChR-A knockdown impairs the learning-associated depression of odor responses in a mushroom body output neuron. Our results suggest that mAChR-A function in Kenyon cell dendrites is required for synaptic plasticity between Kenyon cells and their output neurons.
format Online
Article
Text
id pubmed-6641838
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher eLife Sciences Publications, Ltd
record_format MEDLINE/PubMed
spelling pubmed-66418382019-07-22 Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila Bielopolski, Noa Amin, Hoger Apostolopoulou, Anthi A Rozenfeld, Eyal Lerner, Hadas Huetteroth, Wolf Lin, Andrew C Parnas, Moshe eLife Neuroscience Olfactory associative learning in Drosophila is mediated by synaptic plasticity between the Kenyon cells of the mushroom body and their output neurons. Both Kenyon cells and their inputs from projection neurons are cholinergic, yet little is known about the physiological function of muscarinic acetylcholine receptors in learning in adult flies. Here, we show that aversive olfactory learning in adult flies requires type A muscarinic acetylcholine receptors (mAChR-A), particularly in the gamma subtype of Kenyon cells. mAChR-A inhibits odor responses and is localized in Kenyon cell dendrites. Moreover, mAChR-A knockdown impairs the learning-associated depression of odor responses in a mushroom body output neuron. Our results suggest that mAChR-A function in Kenyon cell dendrites is required for synaptic plasticity between Kenyon cells and their output neurons. eLife Sciences Publications, Ltd 2019-06-19 /pmc/articles/PMC6641838/ /pubmed/31215865 http://dx.doi.org/10.7554/eLife.48264 Text en © 2019, Bielopolski et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Bielopolski, Noa
Amin, Hoger
Apostolopoulou, Anthi A
Rozenfeld, Eyal
Lerner, Hadas
Huetteroth, Wolf
Lin, Andrew C
Parnas, Moshe
Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila
title Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila
title_full Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila
title_fullStr Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila
title_full_unstemmed Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila
title_short Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila
title_sort inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult drosophila
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641838/
https://www.ncbi.nlm.nih.gov/pubmed/31215865
http://dx.doi.org/10.7554/eLife.48264
work_keys_str_mv AT bielopolskinoa inhibitorymuscarinicacetylcholinereceptorsenhanceaversiveolfactorylearninginadultdrosophila
AT aminhoger inhibitorymuscarinicacetylcholinereceptorsenhanceaversiveolfactorylearninginadultdrosophila
AT apostolopoulouanthia inhibitorymuscarinicacetylcholinereceptorsenhanceaversiveolfactorylearninginadultdrosophila
AT rozenfeldeyal inhibitorymuscarinicacetylcholinereceptorsenhanceaversiveolfactorylearninginadultdrosophila
AT lernerhadas inhibitorymuscarinicacetylcholinereceptorsenhanceaversiveolfactorylearninginadultdrosophila
AT huetterothwolf inhibitorymuscarinicacetylcholinereceptorsenhanceaversiveolfactorylearninginadultdrosophila
AT linandrewc inhibitorymuscarinicacetylcholinereceptorsenhanceaversiveolfactorylearninginadultdrosophila
AT parnasmoshe inhibitorymuscarinicacetylcholinereceptorsenhanceaversiveolfactorylearninginadultdrosophila