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Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila
Olfactory associative learning in Drosophila is mediated by synaptic plasticity between the Kenyon cells of the mushroom body and their output neurons. Both Kenyon cells and their inputs from projection neurons are cholinergic, yet little is known about the physiological function of muscarinic acety...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641838/ https://www.ncbi.nlm.nih.gov/pubmed/31215865 http://dx.doi.org/10.7554/eLife.48264 |
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author | Bielopolski, Noa Amin, Hoger Apostolopoulou, Anthi A Rozenfeld, Eyal Lerner, Hadas Huetteroth, Wolf Lin, Andrew C Parnas, Moshe |
author_facet | Bielopolski, Noa Amin, Hoger Apostolopoulou, Anthi A Rozenfeld, Eyal Lerner, Hadas Huetteroth, Wolf Lin, Andrew C Parnas, Moshe |
author_sort | Bielopolski, Noa |
collection | PubMed |
description | Olfactory associative learning in Drosophila is mediated by synaptic plasticity between the Kenyon cells of the mushroom body and their output neurons. Both Kenyon cells and their inputs from projection neurons are cholinergic, yet little is known about the physiological function of muscarinic acetylcholine receptors in learning in adult flies. Here, we show that aversive olfactory learning in adult flies requires type A muscarinic acetylcholine receptors (mAChR-A), particularly in the gamma subtype of Kenyon cells. mAChR-A inhibits odor responses and is localized in Kenyon cell dendrites. Moreover, mAChR-A knockdown impairs the learning-associated depression of odor responses in a mushroom body output neuron. Our results suggest that mAChR-A function in Kenyon cell dendrites is required for synaptic plasticity between Kenyon cells and their output neurons. |
format | Online Article Text |
id | pubmed-6641838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-66418382019-07-22 Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila Bielopolski, Noa Amin, Hoger Apostolopoulou, Anthi A Rozenfeld, Eyal Lerner, Hadas Huetteroth, Wolf Lin, Andrew C Parnas, Moshe eLife Neuroscience Olfactory associative learning in Drosophila is mediated by synaptic plasticity between the Kenyon cells of the mushroom body and their output neurons. Both Kenyon cells and their inputs from projection neurons are cholinergic, yet little is known about the physiological function of muscarinic acetylcholine receptors in learning in adult flies. Here, we show that aversive olfactory learning in adult flies requires type A muscarinic acetylcholine receptors (mAChR-A), particularly in the gamma subtype of Kenyon cells. mAChR-A inhibits odor responses and is localized in Kenyon cell dendrites. Moreover, mAChR-A knockdown impairs the learning-associated depression of odor responses in a mushroom body output neuron. Our results suggest that mAChR-A function in Kenyon cell dendrites is required for synaptic plasticity between Kenyon cells and their output neurons. eLife Sciences Publications, Ltd 2019-06-19 /pmc/articles/PMC6641838/ /pubmed/31215865 http://dx.doi.org/10.7554/eLife.48264 Text en © 2019, Bielopolski et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Bielopolski, Noa Amin, Hoger Apostolopoulou, Anthi A Rozenfeld, Eyal Lerner, Hadas Huetteroth, Wolf Lin, Andrew C Parnas, Moshe Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila |
title | Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila |
title_full | Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila |
title_fullStr | Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila |
title_full_unstemmed | Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila |
title_short | Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila |
title_sort | inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult drosophila |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6641838/ https://www.ncbi.nlm.nih.gov/pubmed/31215865 http://dx.doi.org/10.7554/eLife.48264 |
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