Amplification-free library preparation with SAFE Hi-C uses ligation products for deep sequencing to improve traditional Hi-C analysis

PCR amplification of Hi-C libraries introduces unusable duplicates and results in a biased representation of chromatin interactions. We present a simplified, fast, and economically efficient Hi-C library preparation procedure, SAFE Hi-C, which generates sufficient non-amplified ligation products for...

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Detalles Bibliográficos
Autores principales: Niu, Longjian, Shen, Wei, Huang, Yingzhang, He, Na, Zhang, Yuedong, Sun, Jialei, Wan, Jing, Jiang, Daxin, Yang, Manyun, Tse, Yu Chung, Li, Li, Hou, Chunhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6642088/
https://www.ncbi.nlm.nih.gov/pubmed/31341966
http://dx.doi.org/10.1038/s42003-019-0519-y

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