Cargando…
Prevalence and Genotyping of Echinococcus Species from Livestock in Kajiado County, Kenya
Cystic Echinococcosis (CE) is a widespread neglected zoonotic disease and is caused by the larval stage of the dog tapeworm Echinococcus granulosus sensu lato. CE is more frequent in livestock-rearing areas and where people live a nomadic or seminomadic lifestyle such as in Kajiado County, Kenya. Th...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6642774/ https://www.ncbi.nlm.nih.gov/pubmed/31360712 http://dx.doi.org/10.1155/2019/4798906 |
_version_ | 1783437036815908864 |
---|---|
author | Nungari, Lucy Mbae, Cecilia Gikunju, Joseph Mulinge, Erastus Kaburu, Timothy Zeyhle, Eberhard Magambo, Japhet |
author_facet | Nungari, Lucy Mbae, Cecilia Gikunju, Joseph Mulinge, Erastus Kaburu, Timothy Zeyhle, Eberhard Magambo, Japhet |
author_sort | Nungari, Lucy |
collection | PubMed |
description | Cystic Echinococcosis (CE) is a widespread neglected zoonotic disease and is caused by the larval stage of the dog tapeworm Echinococcus granulosus sensu lato. CE is more frequent in livestock-rearing areas and where people live a nomadic or seminomadic lifestyle such as in Kajiado County, Kenya. There is limited data on CE disease situation in the county of Maasailand; the present study, therefore, reports on the prevalence of CE in cattle, sheep, and goats and their relative importance in CE transmission in Kajiado County. In total, 1,486 livestock (388 cattle, 625 sheep, and 473 goats) slaughtered in two abattoirs were examined for the presence of hydatid cysts in various organs. Cyst isolates were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the NADH dehydrogenase subunit 1 gene (nad1). The overall prevalence of CE was 14.8% (220/1486), while prevalence per livestock species was 15.2% (72/473) in goats, 14.9% (93/625) in sheep, and 14.2% (55/388) in cattle. Out of the 421 cysts isolated, 389 cysts were successfully characterized to be either E. granulosus sensu stricto (s. s.), 356/389 (91.5%), E. canadensis (G6/7), 26/389 (6.7%), or E. ortleppi, 7/389 (1.8%). This record confirms predominance of E. granulosus s. s. in Maasailand and other parts of Kenya, while the importance of E. ortleppi and E. canadensis (G6/7) to the general CE burden in Maasailand might be higher than previously thought. More so, a higher infection pressure for humans by E. granulosus s. s. based on its abundance could be speculated. The study sheds significant light on CE situation in livestock in the nomadic/seminomadic society of the Maasai in Kajiado County and provides good bases to investigate human CE in the area. |
format | Online Article Text |
id | pubmed-6642774 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-66427742019-07-29 Prevalence and Genotyping of Echinococcus Species from Livestock in Kajiado County, Kenya Nungari, Lucy Mbae, Cecilia Gikunju, Joseph Mulinge, Erastus Kaburu, Timothy Zeyhle, Eberhard Magambo, Japhet Biomed Res Int Research Article Cystic Echinococcosis (CE) is a widespread neglected zoonotic disease and is caused by the larval stage of the dog tapeworm Echinococcus granulosus sensu lato. CE is more frequent in livestock-rearing areas and where people live a nomadic or seminomadic lifestyle such as in Kajiado County, Kenya. There is limited data on CE disease situation in the county of Maasailand; the present study, therefore, reports on the prevalence of CE in cattle, sheep, and goats and their relative importance in CE transmission in Kajiado County. In total, 1,486 livestock (388 cattle, 625 sheep, and 473 goats) slaughtered in two abattoirs were examined for the presence of hydatid cysts in various organs. Cyst isolates were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the NADH dehydrogenase subunit 1 gene (nad1). The overall prevalence of CE was 14.8% (220/1486), while prevalence per livestock species was 15.2% (72/473) in goats, 14.9% (93/625) in sheep, and 14.2% (55/388) in cattle. Out of the 421 cysts isolated, 389 cysts were successfully characterized to be either E. granulosus sensu stricto (s. s.), 356/389 (91.5%), E. canadensis (G6/7), 26/389 (6.7%), or E. ortleppi, 7/389 (1.8%). This record confirms predominance of E. granulosus s. s. in Maasailand and other parts of Kenya, while the importance of E. ortleppi and E. canadensis (G6/7) to the general CE burden in Maasailand might be higher than previously thought. More so, a higher infection pressure for humans by E. granulosus s. s. based on its abundance could be speculated. The study sheds significant light on CE situation in livestock in the nomadic/seminomadic society of the Maasai in Kajiado County and provides good bases to investigate human CE in the area. Hindawi 2019-07-07 /pmc/articles/PMC6642774/ /pubmed/31360712 http://dx.doi.org/10.1155/2019/4798906 Text en Copyright © 2019 Lucy Nungari et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Nungari, Lucy Mbae, Cecilia Gikunju, Joseph Mulinge, Erastus Kaburu, Timothy Zeyhle, Eberhard Magambo, Japhet Prevalence and Genotyping of Echinococcus Species from Livestock in Kajiado County, Kenya |
title | Prevalence and Genotyping of Echinococcus Species from Livestock in Kajiado County, Kenya |
title_full | Prevalence and Genotyping of Echinococcus Species from Livestock in Kajiado County, Kenya |
title_fullStr | Prevalence and Genotyping of Echinococcus Species from Livestock in Kajiado County, Kenya |
title_full_unstemmed | Prevalence and Genotyping of Echinococcus Species from Livestock in Kajiado County, Kenya |
title_short | Prevalence and Genotyping of Echinococcus Species from Livestock in Kajiado County, Kenya |
title_sort | prevalence and genotyping of echinococcus species from livestock in kajiado county, kenya |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6642774/ https://www.ncbi.nlm.nih.gov/pubmed/31360712 http://dx.doi.org/10.1155/2019/4798906 |
work_keys_str_mv | AT nungarilucy prevalenceandgenotypingofechinococcusspeciesfromlivestockinkajiadocountykenya AT mbaececilia prevalenceandgenotypingofechinococcusspeciesfromlivestockinkajiadocountykenya AT gikunjujoseph prevalenceandgenotypingofechinococcusspeciesfromlivestockinkajiadocountykenya AT mulingeerastus prevalenceandgenotypingofechinococcusspeciesfromlivestockinkajiadocountykenya AT kaburutimothy prevalenceandgenotypingofechinococcusspeciesfromlivestockinkajiadocountykenya AT zeyhleeberhard prevalenceandgenotypingofechinococcusspeciesfromlivestockinkajiadocountykenya AT magambojaphet prevalenceandgenotypingofechinococcusspeciesfromlivestockinkajiadocountykenya |