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The Pseudogene PTTG3P Promotes Cell Migration and Invasion in Esophageal Squamous Cell Carcinoma
Pseudogenes are pivotal funtional non-coding RNAs in tumorigenesis. Cumulative evidences have shown that pituitary tumor-transforming 3, pseudogene (PTTG3P), serves as an oncogene in multiple human cancers. However, its expression pattern, biological function, and potential targets in esophageal squ...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
De Gruyter
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6642799/ https://www.ncbi.nlm.nih.gov/pubmed/31346548 http://dx.doi.org/10.1515/med-2019-0057 |
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author | Zhang, Zhenhua Shi, Zhengyuan |
author_facet | Zhang, Zhenhua Shi, Zhengyuan |
author_sort | Zhang, Zhenhua |
collection | PubMed |
description | Pseudogenes are pivotal funtional non-coding RNAs in tumorigenesis. Cumulative evidences have shown that pituitary tumor-transforming 3, pseudogene (PTTG3P), serves as an oncogene in multiple human cancers. However, its expression pattern, biological function, and potential targets in esophageal squamous cell carcinoma (ESCC) remain unknown. Here, by quantitative real-time polymerase chain reaction (qRT-PCR) in 50 cases of ESCC, we found that the expression of PTTG3P, PTTG1 and PTTG2 in esophageal squamous cancer tissues and cell lines were significantly higher than their normal counterparts (P<0.01). Spearman correlation analysis showed that the PTTG3P expression was positively correlated with the PTTG1 and PTTG2 expression in ESCC tissue samples (P<0.05). Additionally, the high expression of PTTG3P in ESCC was significantly correlated with tumor depth, lymph node invasion and TNM stage (P<0.05). We also assessed the function of PTTG3P in vitro by gain-of-function studies. Results showed that enhanced expression of PTTG3P stimulated the migration and invasion of ESCC cells, and promoted the expression level of PTTG1 and PTTG2 in vitro. Furthermore, PTTG3P fulfilled its oncogenic functions by positively regulating its parent gene PTTG1 and PTTG2. Overall, our study indicated that PTTG3P is distinctly overexpressed and exhibited oncogenic role in a PTTG1 and PTTG2 mediated manner in ESCC. |
format | Online Article Text |
id | pubmed-6642799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-66427992019-07-25 The Pseudogene PTTG3P Promotes Cell Migration and Invasion in Esophageal Squamous Cell Carcinoma Zhang, Zhenhua Shi, Zhengyuan Open Med (Wars) Research Article Pseudogenes are pivotal funtional non-coding RNAs in tumorigenesis. Cumulative evidences have shown that pituitary tumor-transforming 3, pseudogene (PTTG3P), serves as an oncogene in multiple human cancers. However, its expression pattern, biological function, and potential targets in esophageal squamous cell carcinoma (ESCC) remain unknown. Here, by quantitative real-time polymerase chain reaction (qRT-PCR) in 50 cases of ESCC, we found that the expression of PTTG3P, PTTG1 and PTTG2 in esophageal squamous cancer tissues and cell lines were significantly higher than their normal counterparts (P<0.01). Spearman correlation analysis showed that the PTTG3P expression was positively correlated with the PTTG1 and PTTG2 expression in ESCC tissue samples (P<0.05). Additionally, the high expression of PTTG3P in ESCC was significantly correlated with tumor depth, lymph node invasion and TNM stage (P<0.05). We also assessed the function of PTTG3P in vitro by gain-of-function studies. Results showed that enhanced expression of PTTG3P stimulated the migration and invasion of ESCC cells, and promoted the expression level of PTTG1 and PTTG2 in vitro. Furthermore, PTTG3P fulfilled its oncogenic functions by positively regulating its parent gene PTTG1 and PTTG2. Overall, our study indicated that PTTG3P is distinctly overexpressed and exhibited oncogenic role in a PTTG1 and PTTG2 mediated manner in ESCC. De Gruyter 2019-06-30 /pmc/articles/PMC6642799/ /pubmed/31346548 http://dx.doi.org/10.1515/med-2019-0057 Text en © 2019 Zhenhua Zhang, Zhengyuan Shi, published by De Gruyter http://creativecommons.org/licenses/by-nc-nd/4.0 This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. |
spellingShingle | Research Article Zhang, Zhenhua Shi, Zhengyuan The Pseudogene PTTG3P Promotes Cell Migration and Invasion in Esophageal Squamous Cell Carcinoma |
title | The Pseudogene PTTG3P Promotes Cell Migration and Invasion in Esophageal Squamous Cell Carcinoma |
title_full | The Pseudogene PTTG3P Promotes Cell Migration and Invasion in Esophageal Squamous Cell Carcinoma |
title_fullStr | The Pseudogene PTTG3P Promotes Cell Migration and Invasion in Esophageal Squamous Cell Carcinoma |
title_full_unstemmed | The Pseudogene PTTG3P Promotes Cell Migration and Invasion in Esophageal Squamous Cell Carcinoma |
title_short | The Pseudogene PTTG3P Promotes Cell Migration and Invasion in Esophageal Squamous Cell Carcinoma |
title_sort | pseudogene pttg3p promotes cell migration and invasion in esophageal squamous cell carcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6642799/ https://www.ncbi.nlm.nih.gov/pubmed/31346548 http://dx.doi.org/10.1515/med-2019-0057 |
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