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The interaction of silica nanoparticles with catalase and human mesenchymal stem cells: biophysical, theoretical and cellular studies
AIM: Nanoparticles (NPs) have been receiving potential interests in protein delivery and cell therapy. As a matter of fact, NPs may be used as great candidates in promoting cell therapy by catalase (CAT) delivery into high oxidative stress tissues. However, for using NPs like SiO(2) as carriers, the...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643057/ https://www.ncbi.nlm.nih.gov/pubmed/31409992 http://dx.doi.org/10.2147/IJN.S210136 |
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author | Mousavi, Mina Hakimian, Saman Mustafa, Twana Ahmed Aziz, Falah Mohammad Salihi, Abbas Ale-Ebrahim, Mahsa Mirpour, Mirsasan Rasti, Behnam Akhtari, Keivan Shahpasand, Koorosh Abou-Zied, Osama K Falahati, Mojtaba |
author_facet | Mousavi, Mina Hakimian, Saman Mustafa, Twana Ahmed Aziz, Falah Mohammad Salihi, Abbas Ale-Ebrahim, Mahsa Mirpour, Mirsasan Rasti, Behnam Akhtari, Keivan Shahpasand, Koorosh Abou-Zied, Osama K Falahati, Mojtaba |
author_sort | Mousavi, Mina |
collection | PubMed |
description | AIM: Nanoparticles (NPs) have been receiving potential interests in protein delivery and cell therapy. As a matter of fact, NPs may be used as great candidates in promoting cell therapy by catalase (CAT) delivery into high oxidative stress tissues. However, for using NPs like SiO(2) as carriers, the interaction of NPs with proteins and mesenchymal stem cells (MSCs) should be explored in advance. METHODS: In the present study, the interaction of SiO(2) NPs with CAT and human MSCs (hMSCs) was explored by various spectroscopic methods (fluorescence, circular dichroism (CD), UV-visible), molecular docking and dynamics studies, and cellular (MTT, cellular morphology, cellular uptake, lactate dehydrogenase, ROS, caspase-3, flow cytometry) assays. RESULTS: Fluorescence study displayed that both dynamic and static quenching mechanisms and hydrophobic interactions are involved in the spontaneous interaction of SiO(2) NPs with CAT. CD spectra indicated that native structure of CAT remains stable after interaction with SiO(2) NPs. UV-visible study also revealed that the kinetic parameters of CAT such as Km, Vmax, Kcat, and enzyme efficiency were not changed after the addition of SiO(2) NPs. Molecular docking and dynamics studies showed that Si and SiO(2) clusters interact with hydrophobic residues of CAT and SiO(2) cluster causes minor changes in the CAT structure at a total simulation time of 200 ps. Cellular assays depicted that SiO(2) NPs induce significant cell mortality, change in cellular morphology, cellular internalization, ROS elevation, and apoptosis in hMSCs at higher concentration than 100 µg/mL (170 µM). CONCLUSION: The current results suggest that low concentrations of SiO(2) NPs induce no substantial change or mortality against CAT and hMSCs, and potentially useful carriers in CAT delivery to hMSC. |
format | Online Article Text |
id | pubmed-6643057 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-66430572019-08-13 The interaction of silica nanoparticles with catalase and human mesenchymal stem cells: biophysical, theoretical and cellular studies Mousavi, Mina Hakimian, Saman Mustafa, Twana Ahmed Aziz, Falah Mohammad Salihi, Abbas Ale-Ebrahim, Mahsa Mirpour, Mirsasan Rasti, Behnam Akhtari, Keivan Shahpasand, Koorosh Abou-Zied, Osama K Falahati, Mojtaba Int J Nanomedicine Original Research AIM: Nanoparticles (NPs) have been receiving potential interests in protein delivery and cell therapy. As a matter of fact, NPs may be used as great candidates in promoting cell therapy by catalase (CAT) delivery into high oxidative stress tissues. However, for using NPs like SiO(2) as carriers, the interaction of NPs with proteins and mesenchymal stem cells (MSCs) should be explored in advance. METHODS: In the present study, the interaction of SiO(2) NPs with CAT and human MSCs (hMSCs) was explored by various spectroscopic methods (fluorescence, circular dichroism (CD), UV-visible), molecular docking and dynamics studies, and cellular (MTT, cellular morphology, cellular uptake, lactate dehydrogenase, ROS, caspase-3, flow cytometry) assays. RESULTS: Fluorescence study displayed that both dynamic and static quenching mechanisms and hydrophobic interactions are involved in the spontaneous interaction of SiO(2) NPs with CAT. CD spectra indicated that native structure of CAT remains stable after interaction with SiO(2) NPs. UV-visible study also revealed that the kinetic parameters of CAT such as Km, Vmax, Kcat, and enzyme efficiency were not changed after the addition of SiO(2) NPs. Molecular docking and dynamics studies showed that Si and SiO(2) clusters interact with hydrophobic residues of CAT and SiO(2) cluster causes minor changes in the CAT structure at a total simulation time of 200 ps. Cellular assays depicted that SiO(2) NPs induce significant cell mortality, change in cellular morphology, cellular internalization, ROS elevation, and apoptosis in hMSCs at higher concentration than 100 µg/mL (170 µM). CONCLUSION: The current results suggest that low concentrations of SiO(2) NPs induce no substantial change or mortality against CAT and hMSCs, and potentially useful carriers in CAT delivery to hMSC. Dove 2019-07-16 /pmc/articles/PMC6643057/ /pubmed/31409992 http://dx.doi.org/10.2147/IJN.S210136 Text en © 2019 Mousavi et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Mousavi, Mina Hakimian, Saman Mustafa, Twana Ahmed Aziz, Falah Mohammad Salihi, Abbas Ale-Ebrahim, Mahsa Mirpour, Mirsasan Rasti, Behnam Akhtari, Keivan Shahpasand, Koorosh Abou-Zied, Osama K Falahati, Mojtaba The interaction of silica nanoparticles with catalase and human mesenchymal stem cells: biophysical, theoretical and cellular studies |
title | The interaction of silica nanoparticles with catalase and human mesenchymal stem cells: biophysical, theoretical and cellular studies |
title_full | The interaction of silica nanoparticles with catalase and human mesenchymal stem cells: biophysical, theoretical and cellular studies |
title_fullStr | The interaction of silica nanoparticles with catalase and human mesenchymal stem cells: biophysical, theoretical and cellular studies |
title_full_unstemmed | The interaction of silica nanoparticles with catalase and human mesenchymal stem cells: biophysical, theoretical and cellular studies |
title_short | The interaction of silica nanoparticles with catalase and human mesenchymal stem cells: biophysical, theoretical and cellular studies |
title_sort | interaction of silica nanoparticles with catalase and human mesenchymal stem cells: biophysical, theoretical and cellular studies |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643057/ https://www.ncbi.nlm.nih.gov/pubmed/31409992 http://dx.doi.org/10.2147/IJN.S210136 |
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