Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor

[Image: see text] The stability, reusability, and monitoring of enzyme activity have been investigated to improve their efficiency for successful utilization in a broad range of industrial and medical applications. Herein, we present a simple method for fabricating an electrospun fiber/enzyme scaffo...

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Autores principales: Aldhahri, Musab M., Almulaiky, Yaaser Q., El-Shishtawy, Reda M., Al-Shawafi, Waleed, Alngadh, Ahmed, Maghrabi, Rayan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6644564/
https://www.ncbi.nlm.nih.gov/pubmed/31458817
http://dx.doi.org/10.1021/acsomega.8b00366
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author Aldhahri, Musab M.
Almulaiky, Yaaser Q.
El-Shishtawy, Reda M.
Al-Shawafi, Waleed
Alngadh, Ahmed
Maghrabi, Rayan
author_facet Aldhahri, Musab M.
Almulaiky, Yaaser Q.
El-Shishtawy, Reda M.
Al-Shawafi, Waleed
Alngadh, Ahmed
Maghrabi, Rayan
author_sort Aldhahri, Musab M.
collection PubMed
description [Image: see text] The stability, reusability, and monitoring of enzyme activity have been investigated to improve their efficiency for successful utilization in a broad range of industrial and medical applications. Herein, we present a simple method for fabricating an electrospun fiber/enzyme scaffold via co-electrospinning. The characterization of soluble and immobilized α-amylases with regard to pH, thermal stability, and reusability were studied. An organic light emitting material tris(8-hydroxyquinoline)aluminum was incorporated to monitor the enzyme activity for several reuses.
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spelling pubmed-66445642019-08-27 Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor Aldhahri, Musab M. Almulaiky, Yaaser Q. El-Shishtawy, Reda M. Al-Shawafi, Waleed Alngadh, Ahmed Maghrabi, Rayan ACS Omega [Image: see text] The stability, reusability, and monitoring of enzyme activity have been investigated to improve their efficiency for successful utilization in a broad range of industrial and medical applications. Herein, we present a simple method for fabricating an electrospun fiber/enzyme scaffold via co-electrospinning. The characterization of soluble and immobilized α-amylases with regard to pH, thermal stability, and reusability were studied. An organic light emitting material tris(8-hydroxyquinoline)aluminum was incorporated to monitor the enzyme activity for several reuses. American Chemical Society 2018-06-13 /pmc/articles/PMC6644564/ /pubmed/31458817 http://dx.doi.org/10.1021/acsomega.8b00366 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Aldhahri, Musab M.
Almulaiky, Yaaser Q.
El-Shishtawy, Reda M.
Al-Shawafi, Waleed
Alngadh, Ahmed
Maghrabi, Rayan
Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor
title Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor
title_full Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor
title_fullStr Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor
title_full_unstemmed Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor
title_short Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor
title_sort facile immobilization of enzyme via co-electrospinning: a simple method for enhancing enzyme reusability and monitoring an activity-based organic semiconductor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6644564/
https://www.ncbi.nlm.nih.gov/pubmed/31458817
http://dx.doi.org/10.1021/acsomega.8b00366
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