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Redox Modulators Determine Luminol Luminescence Generated by Porphyrin-Coordinated Iron and May Repress “Suicide Inactivation”

[Image: see text] Iron porphyrin catalysts of the luminol reaction (horseradish peroxidase, hemoglobin, cytochrome c, and hemin) interact with diverse reducing compounds. Here, it is demonstrated how the chemiluminescence yield is modulated by such interactions. The compounds accepted as substrates...

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Autor principal: Plieth, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6645248/
https://www.ncbi.nlm.nih.gov/pubmed/31459303
http://dx.doi.org/10.1021/acsomega.8b01261
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author Plieth, Christoph
author_facet Plieth, Christoph
author_sort Plieth, Christoph
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description [Image: see text] Iron porphyrin catalysts of the luminol reaction (horseradish peroxidase, hemoglobin, cytochrome c, and hemin) interact with diverse reducing compounds. Here, it is demonstrated how the chemiluminescence yield is modulated by such interactions. The compounds accepted as substrates protect the catalyst against the “suicide inactivation” caused by high peroxide concentrations. The reducing agents not accepted by the catalyst inhibit light production either by generating a futile redox cycle of the luminophore or by irreversibly inactivating the catalytic center. In the case of a futile cycle, light emission resumes as soon as the reducing agents in the reaction are consumed, whereas with an irreversible inactivation, light emission does not recover. The characteristics of luminescence enhancement and quenching depending on interfering agents are also reported here. They reveal details about the relative redox potentials of the involved compounds. It is discussed how this should be considered when the luminol reaction is used for quantitative analyses and when unpurified samples with a broad compound matrix are to be assayed.
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spelling pubmed-66452482019-08-27 Redox Modulators Determine Luminol Luminescence Generated by Porphyrin-Coordinated Iron and May Repress “Suicide Inactivation” Plieth, Christoph ACS Omega [Image: see text] Iron porphyrin catalysts of the luminol reaction (horseradish peroxidase, hemoglobin, cytochrome c, and hemin) interact with diverse reducing compounds. Here, it is demonstrated how the chemiluminescence yield is modulated by such interactions. The compounds accepted as substrates protect the catalyst against the “suicide inactivation” caused by high peroxide concentrations. The reducing agents not accepted by the catalyst inhibit light production either by generating a futile redox cycle of the luminophore or by irreversibly inactivating the catalytic center. In the case of a futile cycle, light emission resumes as soon as the reducing agents in the reaction are consumed, whereas with an irreversible inactivation, light emission does not recover. The characteristics of luminescence enhancement and quenching depending on interfering agents are also reported here. They reveal details about the relative redox potentials of the involved compounds. It is discussed how this should be considered when the luminol reaction is used for quantitative analyses and when unpurified samples with a broad compound matrix are to be assayed. American Chemical Society 2018-09-28 /pmc/articles/PMC6645248/ /pubmed/31459303 http://dx.doi.org/10.1021/acsomega.8b01261 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Plieth, Christoph
Redox Modulators Determine Luminol Luminescence Generated by Porphyrin-Coordinated Iron and May Repress “Suicide Inactivation”
title Redox Modulators Determine Luminol Luminescence Generated by Porphyrin-Coordinated Iron and May Repress “Suicide Inactivation”
title_full Redox Modulators Determine Luminol Luminescence Generated by Porphyrin-Coordinated Iron and May Repress “Suicide Inactivation”
title_fullStr Redox Modulators Determine Luminol Luminescence Generated by Porphyrin-Coordinated Iron and May Repress “Suicide Inactivation”
title_full_unstemmed Redox Modulators Determine Luminol Luminescence Generated by Porphyrin-Coordinated Iron and May Repress “Suicide Inactivation”
title_short Redox Modulators Determine Luminol Luminescence Generated by Porphyrin-Coordinated Iron and May Repress “Suicide Inactivation”
title_sort redox modulators determine luminol luminescence generated by porphyrin-coordinated iron and may repress “suicide inactivation”
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6645248/
https://www.ncbi.nlm.nih.gov/pubmed/31459303
http://dx.doi.org/10.1021/acsomega.8b01261
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