Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice

Thy28, also known as ThyN1, is a highly conserved nuclear protein. We previously showed that in a chicken mature B cell line, Thy28 binds to the promoter of the gene encoding Pax5, a transcription factor essential for B cell development, and positively regulates its expression. Here, we generated a...

Descripción completa

Detalles Bibliográficos
Autores principales: Kitaura, Fusako, Yuno, Miyuki, Fujita, Toshitsugu, Wakana, Shigeharu, Ueda, Jun, Yamagata, Kazuo, Fujii, Hodaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6645560/
https://www.ncbi.nlm.nih.gov/pubmed/31329649
http://dx.doi.org/10.1371/journal.pone.0220199
_version_ 1783437488249896960
author Kitaura, Fusako
Yuno, Miyuki
Fujita, Toshitsugu
Wakana, Shigeharu
Ueda, Jun
Yamagata, Kazuo
Fujii, Hodaka
author_facet Kitaura, Fusako
Yuno, Miyuki
Fujita, Toshitsugu
Wakana, Shigeharu
Ueda, Jun
Yamagata, Kazuo
Fujii, Hodaka
author_sort Kitaura, Fusako
collection PubMed
description Thy28, also known as ThyN1, is a highly conserved nuclear protein. We previously showed that in a chicken mature B cell line, Thy28 binds to the promoter of the gene encoding Pax5, a transcription factor essential for B cell development, and positively regulates its expression. Here, we generated a Thy28-deficient mouse line to analyze its potential role in B cell development in mice. Thy28-deficient mice showed normal development of B cells, and the expression of Pax5 was comparable between wild-type and Thy28-deficient primary B cells. Thus, species-specific mechanisms regulate Pax5 expression and B cell development.
format Online
Article
Text
id pubmed-6645560
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-66455602019-07-25 Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice Kitaura, Fusako Yuno, Miyuki Fujita, Toshitsugu Wakana, Shigeharu Ueda, Jun Yamagata, Kazuo Fujii, Hodaka PLoS One Research Article Thy28, also known as ThyN1, is a highly conserved nuclear protein. We previously showed that in a chicken mature B cell line, Thy28 binds to the promoter of the gene encoding Pax5, a transcription factor essential for B cell development, and positively regulates its expression. Here, we generated a Thy28-deficient mouse line to analyze its potential role in B cell development in mice. Thy28-deficient mice showed normal development of B cells, and the expression of Pax5 was comparable between wild-type and Thy28-deficient primary B cells. Thus, species-specific mechanisms regulate Pax5 expression and B cell development. Public Library of Science 2019-07-22 /pmc/articles/PMC6645560/ /pubmed/31329649 http://dx.doi.org/10.1371/journal.pone.0220199 Text en © 2019 Kitaura et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kitaura, Fusako
Yuno, Miyuki
Fujita, Toshitsugu
Wakana, Shigeharu
Ueda, Jun
Yamagata, Kazuo
Fujii, Hodaka
Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice
title Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice
title_full Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice
title_fullStr Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice
title_full_unstemmed Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice
title_short Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice
title_sort normal b cell development and pax5 expression in thy28/thyn1-deficient mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6645560/
https://www.ncbi.nlm.nih.gov/pubmed/31329649
http://dx.doi.org/10.1371/journal.pone.0220199
work_keys_str_mv AT kitaurafusako normalbcelldevelopmentandpax5expressioninthy28thyn1deficientmice
AT yunomiyuki normalbcelldevelopmentandpax5expressioninthy28thyn1deficientmice
AT fujitatoshitsugu normalbcelldevelopmentandpax5expressioninthy28thyn1deficientmice
AT wakanashigeharu normalbcelldevelopmentandpax5expressioninthy28thyn1deficientmice
AT uedajun normalbcelldevelopmentandpax5expressioninthy28thyn1deficientmice
AT yamagatakazuo normalbcelldevelopmentandpax5expressioninthy28thyn1deficientmice
AT fujiihodaka normalbcelldevelopmentandpax5expressioninthy28thyn1deficientmice

Ejemplares similares