Phenotypic and Functional Characterization of Müller Glia Isolated from Induced Pluripotent Stem Cell‐Derived Retinal Organoids: Improvement of Retinal Ganglion Cell Function upon Transplantation

Glaucoma is one of the leading causes of blindness, and there is an ongoing need for new therapies. Recent studies indicate that cell transplantation using Müller glia may be beneficial, but there is a need for novel sources of cells to provide therapeutic benefit. In this study, we have isolated Mü...

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Autores principales: Eastlake, Karen, Wang, Weixin, Jayaram, Hari, Murray‐Dunning, Celia, Carr, Amanda J. F., Ramsden, Conor M., Vugler, Anthony, Gore, Katrina, Clemo, Nadine, Stewart, Mark, Coffey, Pete, Khaw, Peng T., Limb, G. Astrid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2019
Materias:
Tissue‐Specific Progenitor and Stem Cells
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6646702/
https://www.ncbi.nlm.nih.gov/pubmed/31037833
http://dx.doi.org/10.1002/sctm.18-0263
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author Eastlake, Karen
Wang, Weixin
Jayaram, Hari
Murray‐Dunning, Celia
Carr, Amanda J. F.
Ramsden, Conor M.
Vugler, Anthony
Gore, Katrina
Clemo, Nadine
Stewart, Mark
Coffey, Pete
Khaw, Peng T.
Limb, G. Astrid
author_facet Eastlake, Karen
Wang, Weixin
Jayaram, Hari
Murray‐Dunning, Celia
Carr, Amanda J. F.
Ramsden, Conor M.
Vugler, Anthony
Gore, Katrina
Clemo, Nadine
Stewart, Mark
Coffey, Pete
Khaw, Peng T.
Limb, G. Astrid
author_sort Eastlake, Karen
collection PubMed
description Glaucoma is one of the leading causes of blindness, and there is an ongoing need for new therapies. Recent studies indicate that cell transplantation using Müller glia may be beneficial, but there is a need for novel sources of cells to provide therapeutic benefit. In this study, we have isolated Müller glia from retinal organoids formed by human induced pluripotent stem cells (hiPSCs) in vitro and have shown their ability to partially restore visual function in rats depleted of retinal ganglion cells by NMDA. Based on the present results, we suggest that Müller glia derived from retinal organoids formed by hiPSC may provide an attractive source of cells for human retinal therapies, to prevent and treat vision loss caused by retinal degenerative conditions. stem cells translational medicine 2019;8:775&784
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spelling pubmed-66467022019-07-31 Phenotypic and Functional Characterization of Müller Glia Isolated from Induced Pluripotent Stem Cell‐Derived Retinal Organoids: Improvement of Retinal Ganglion Cell Function upon Transplantation Eastlake, Karen Wang, Weixin Jayaram, Hari Murray‐Dunning, Celia Carr, Amanda J. F. Ramsden, Conor M. Vugler, Anthony Gore, Katrina Clemo, Nadine Stewart, Mark Coffey, Pete Khaw, Peng T. Limb, G. Astrid Stem Cells Transl Med Tissue‐Specific Progenitor and Stem Cells Glaucoma is one of the leading causes of blindness, and there is an ongoing need for new therapies. Recent studies indicate that cell transplantation using Müller glia may be beneficial, but there is a need for novel sources of cells to provide therapeutic benefit. In this study, we have isolated Müller glia from retinal organoids formed by human induced pluripotent stem cells (hiPSCs) in vitro and have shown their ability to partially restore visual function in rats depleted of retinal ganglion cells by NMDA. Based on the present results, we suggest that Müller glia derived from retinal organoids formed by hiPSC may provide an attractive source of cells for human retinal therapies, to prevent and treat vision loss caused by retinal degenerative conditions. stem cells translational medicine 2019;8:775&784 John Wiley & Sons, Inc. 2019-04-29 /pmc/articles/PMC6646702/ /pubmed/31037833 http://dx.doi.org/10.1002/sctm.18-0263 Text en © 2019 The Authors. stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Tissue‐Specific Progenitor and Stem Cells
Eastlake, Karen
Wang, Weixin
Jayaram, Hari
Murray‐Dunning, Celia
Carr, Amanda J. F.
Ramsden, Conor M.
Vugler, Anthony
Gore, Katrina
Clemo, Nadine
Stewart, Mark
Coffey, Pete
Khaw, Peng T.
Limb, G. Astrid
Phenotypic and Functional Characterization of Müller Glia Isolated from Induced Pluripotent Stem Cell‐Derived Retinal Organoids: Improvement of Retinal Ganglion Cell Function upon Transplantation
title Phenotypic and Functional Characterization of Müller Glia Isolated from Induced Pluripotent Stem Cell‐Derived Retinal Organoids: Improvement of Retinal Ganglion Cell Function upon Transplantation
title_full Phenotypic and Functional Characterization of Müller Glia Isolated from Induced Pluripotent Stem Cell‐Derived Retinal Organoids: Improvement of Retinal Ganglion Cell Function upon Transplantation
title_fullStr Phenotypic and Functional Characterization of Müller Glia Isolated from Induced Pluripotent Stem Cell‐Derived Retinal Organoids: Improvement of Retinal Ganglion Cell Function upon Transplantation
title_full_unstemmed Phenotypic and Functional Characterization of Müller Glia Isolated from Induced Pluripotent Stem Cell‐Derived Retinal Organoids: Improvement of Retinal Ganglion Cell Function upon Transplantation
title_short Phenotypic and Functional Characterization of Müller Glia Isolated from Induced Pluripotent Stem Cell‐Derived Retinal Organoids: Improvement of Retinal Ganglion Cell Function upon Transplantation
title_sort phenotypic and functional characterization of müller glia isolated from induced pluripotent stem cell‐derived retinal organoids: improvement of retinal ganglion cell function upon transplantation
topic Tissue‐Specific Progenitor and Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6646702/
https://www.ncbi.nlm.nih.gov/pubmed/31037833
http://dx.doi.org/10.1002/sctm.18-0263
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