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Identification of a TLR2 Inhibiting Wheat Hydrolysate

SCOPE: Wheat hydrolysates are used in medical nutrition to provide undernourished patients a readily digestible protein source, for instance to recover from chemotherapy‐induced intestinal mucosal inflammation. Since many hydrolysates of different sources can modulate the immune system, likely via T...

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Autores principales: Kiewiet, Mensiena B. G., Dekkers, Renske, van Gool, Martine P., Ulfman, Laurien H., Groeneveld, Andre, Faas, Marijke M., de Vos, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6646915/
https://www.ncbi.nlm.nih.gov/pubmed/30354027
http://dx.doi.org/10.1002/mnfr.201800716
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author Kiewiet, Mensiena B. G.
Dekkers, Renske
van Gool, Martine P.
Ulfman, Laurien H.
Groeneveld, Andre
Faas, Marijke M.
de Vos, Paul
author_facet Kiewiet, Mensiena B. G.
Dekkers, Renske
van Gool, Martine P.
Ulfman, Laurien H.
Groeneveld, Andre
Faas, Marijke M.
de Vos, Paul
author_sort Kiewiet, Mensiena B. G.
collection PubMed
description SCOPE: Wheat hydrolysates are used in medical nutrition to provide undernourished patients a readily digestible protein source, for instance to recover from chemotherapy‐induced intestinal mucosal inflammation. Since many hydrolysates of different sources can modulate the immune system, likely via Toll‐like receptors (TLRs), it is hypothesized that also wheat hydrolysates might interact with TLR signaling, which could be a way to prevent intestinal inflammation and damage. METHODS AND RESULTS: The capacity of three wheat hydrolysates to modulate immunity by interfering with TLR signaling is determined. All wheat hydrolysates have TLR modulating effects but only one has strong TLR2 inhibiting effects, attenuating both TLR2/1 and TLR2/6 signaling in a reporter cell system. This is likely induced by direct TLR2‐ectodomain binding, as confirmed by ELISA. Furthermore, this TLR2 blocking hydrolysate reduces IL‐6 production in human dendritic cells. Application of reversed‐phase–ultra HPLC combined with MS reveals that the presence of peptide WQIPEQSR is associated with the observed TLR2 inhibiting capacity. CONCLUSION: The study demonstrates TLR2‐inhibiting capacities of a wheat hydrolysate. The findings provide a good start for further research to investigate whether this hydrolysate might contribute to the management of intestinal mucosal inflammation in cancer patients receiving chemotherapy.
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spelling pubmed-66469152019-07-31 Identification of a TLR2 Inhibiting Wheat Hydrolysate Kiewiet, Mensiena B. G. Dekkers, Renske van Gool, Martine P. Ulfman, Laurien H. Groeneveld, Andre Faas, Marijke M. de Vos, Paul Mol Nutr Food Res Research Articles SCOPE: Wheat hydrolysates are used in medical nutrition to provide undernourished patients a readily digestible protein source, for instance to recover from chemotherapy‐induced intestinal mucosal inflammation. Since many hydrolysates of different sources can modulate the immune system, likely via Toll‐like receptors (TLRs), it is hypothesized that also wheat hydrolysates might interact with TLR signaling, which could be a way to prevent intestinal inflammation and damage. METHODS AND RESULTS: The capacity of three wheat hydrolysates to modulate immunity by interfering with TLR signaling is determined. All wheat hydrolysates have TLR modulating effects but only one has strong TLR2 inhibiting effects, attenuating both TLR2/1 and TLR2/6 signaling in a reporter cell system. This is likely induced by direct TLR2‐ectodomain binding, as confirmed by ELISA. Furthermore, this TLR2 blocking hydrolysate reduces IL‐6 production in human dendritic cells. Application of reversed‐phase–ultra HPLC combined with MS reveals that the presence of peptide WQIPEQSR is associated with the observed TLR2 inhibiting capacity. CONCLUSION: The study demonstrates TLR2‐inhibiting capacities of a wheat hydrolysate. The findings provide a good start for further research to investigate whether this hydrolysate might contribute to the management of intestinal mucosal inflammation in cancer patients receiving chemotherapy. John Wiley and Sons Inc. 2018-11-02 2018-12 /pmc/articles/PMC6646915/ /pubmed/30354027 http://dx.doi.org/10.1002/mnfr.201800716 Text en © 2018 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Kiewiet, Mensiena B. G.
Dekkers, Renske
van Gool, Martine P.
Ulfman, Laurien H.
Groeneveld, Andre
Faas, Marijke M.
de Vos, Paul
Identification of a TLR2 Inhibiting Wheat Hydrolysate
title Identification of a TLR2 Inhibiting Wheat Hydrolysate
title_full Identification of a TLR2 Inhibiting Wheat Hydrolysate
title_fullStr Identification of a TLR2 Inhibiting Wheat Hydrolysate
title_full_unstemmed Identification of a TLR2 Inhibiting Wheat Hydrolysate
title_short Identification of a TLR2 Inhibiting Wheat Hydrolysate
title_sort identification of a tlr2 inhibiting wheat hydrolysate
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6646915/
https://www.ncbi.nlm.nih.gov/pubmed/30354027
http://dx.doi.org/10.1002/mnfr.201800716
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