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Spectrokinetic characterization of photoactive yellow protein films for integrated optical applications

In this paper, the photocycle of the dried photoactive yellow protein film has been investigated in different humidity environments, in order to characterize its nonlinear optical properties for possible integrated optical applications. The light-induced spectral changes of the protein films were mo...

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Autores principales: Krekic, Szilvia, Nagy, Dávid, Taneva, Stefka G., Fábián, László, Zimányi, László, Dér, András
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6647221/
https://www.ncbi.nlm.nih.gov/pubmed/30905045
http://dx.doi.org/10.1007/s00249-019-01353-8
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author Krekic, Szilvia
Nagy, Dávid
Taneva, Stefka G.
Fábián, László
Zimányi, László
Dér, András
author_facet Krekic, Szilvia
Nagy, Dávid
Taneva, Stefka G.
Fábián, László
Zimányi, László
Dér, András
author_sort Krekic, Szilvia
collection PubMed
description In this paper, the photocycle of the dried photoactive yellow protein film has been investigated in different humidity environments, in order to characterize its nonlinear optical properties for possible integrated optical applications. The light-induced spectral changes of the protein films were monitored by an optical multichannel analyser set-up, while the accompanying refractive index changes were measured with the optical waveguide lightmode spectroscopy method. To determine the number and kinetics of spectral intermediates in the photocycle, the absorption kinetic data were analysed by singular value decomposition and multiexponential fitting methods, whose results were used in a subsequent step of fitting a photocycle model to the data. The absorption signals of the films were found to be in strong correlation with the measured light-induced refractive index changes, whose size and kinetics imply that photoactive yellow protein may be a good alternative for utilization as an active nonlinear optical material in future integrated optical applications.
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spelling pubmed-66472212019-08-06 Spectrokinetic characterization of photoactive yellow protein films for integrated optical applications Krekic, Szilvia Nagy, Dávid Taneva, Stefka G. Fábián, László Zimányi, László Dér, András Eur Biophys J Original Article In this paper, the photocycle of the dried photoactive yellow protein film has been investigated in different humidity environments, in order to characterize its nonlinear optical properties for possible integrated optical applications. The light-induced spectral changes of the protein films were monitored by an optical multichannel analyser set-up, while the accompanying refractive index changes were measured with the optical waveguide lightmode spectroscopy method. To determine the number and kinetics of spectral intermediates in the photocycle, the absorption kinetic data were analysed by singular value decomposition and multiexponential fitting methods, whose results were used in a subsequent step of fitting a photocycle model to the data. The absorption signals of the films were found to be in strong correlation with the measured light-induced refractive index changes, whose size and kinetics imply that photoactive yellow protein may be a good alternative for utilization as an active nonlinear optical material in future integrated optical applications. Springer International Publishing 2019-03-23 2019 /pmc/articles/PMC6647221/ /pubmed/30905045 http://dx.doi.org/10.1007/s00249-019-01353-8 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Krekic, Szilvia
Nagy, Dávid
Taneva, Stefka G.
Fábián, László
Zimányi, László
Dér, András
Spectrokinetic characterization of photoactive yellow protein films for integrated optical applications
title Spectrokinetic characterization of photoactive yellow protein films for integrated optical applications
title_full Spectrokinetic characterization of photoactive yellow protein films for integrated optical applications
title_fullStr Spectrokinetic characterization of photoactive yellow protein films for integrated optical applications
title_full_unstemmed Spectrokinetic characterization of photoactive yellow protein films for integrated optical applications
title_short Spectrokinetic characterization of photoactive yellow protein films for integrated optical applications
title_sort spectrokinetic characterization of photoactive yellow protein films for integrated optical applications
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6647221/
https://www.ncbi.nlm.nih.gov/pubmed/30905045
http://dx.doi.org/10.1007/s00249-019-01353-8
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