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Follicular dendritic cells display microvesicle-associated LMP1 in reactive germinal centers of EBV+ classic Hodgkin lymphoma

Expression of the latent membrane protein-1 (LMP1) of Epstein-Barr virus (EBV) was investigated in 153 cases of EBV+ classic Hodgkin lymphoma (cHL); 120 cases were pediatric patients (< 14 years of age) from Iraq, and 33 cases were adult patients from Italy. We describe for the first time the pre...

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Autores principales: Uccini, Stefania, Al-Jadiry, Mazin F., Pepe, Giuseppina, Pasquini, Anna, Alsaadawi, Adel R., Al-Hadad, Salma A., Di Napoli, Arianna, Tripodo, Claudio, Ruco, Luigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6647529/
https://www.ncbi.nlm.nih.gov/pubmed/31203443
http://dx.doi.org/10.1007/s00428-019-02605-w
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author Uccini, Stefania
Al-Jadiry, Mazin F.
Pepe, Giuseppina
Pasquini, Anna
Alsaadawi, Adel R.
Al-Hadad, Salma A.
Di Napoli, Arianna
Tripodo, Claudio
Ruco, Luigi
author_facet Uccini, Stefania
Al-Jadiry, Mazin F.
Pepe, Giuseppina
Pasquini, Anna
Alsaadawi, Adel R.
Al-Hadad, Salma A.
Di Napoli, Arianna
Tripodo, Claudio
Ruco, Luigi
author_sort Uccini, Stefania
collection PubMed
description Expression of the latent membrane protein-1 (LMP1) of Epstein-Barr virus (EBV) was investigated in 153 cases of EBV+ classic Hodgkin lymphoma (cHL); 120 cases were pediatric patients (< 14 years of age) from Iraq, and 33 cases were adult patients from Italy. We describe for the first time the presence of LMP1 protein in EBV-encoded RNA (EBER)-negative follicular dendritic cells (FDCs) of reactive germinal centers (GC) associated with EBV+ cHL. Presence of LMP1+ GCs was independent of geographic region and age of patients. Variable numbers of reactive GCs were present in 22.2% of cases (34 of 153), whereas LMP1 staining of FDCs was present in about a third of cases (10 of 34) with reactive GC. Most cases with LMP1+ GC were mixed-cellularity (MC) subtype, but some nodular sclerosis (NS) was also present. GC cells with LMP1+ FDCs were surrounded by numerous EBV-infected cells which were positive for EBER, LMP1, and CD30. Double immunolocalization analysis revealed that LMP1 was associated with CD63, an exosomal marker, and with CD21. The possibility is discussed that peri-follicular EBV-infected cells release LMP1 protein, perhaps through exosomes, and that the protein is then captured by FDCs and is presented to EBER-negative GC B cells.
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spelling pubmed-66475292019-08-06 Follicular dendritic cells display microvesicle-associated LMP1 in reactive germinal centers of EBV+ classic Hodgkin lymphoma Uccini, Stefania Al-Jadiry, Mazin F. Pepe, Giuseppina Pasquini, Anna Alsaadawi, Adel R. Al-Hadad, Salma A. Di Napoli, Arianna Tripodo, Claudio Ruco, Luigi Virchows Arch Original Article Expression of the latent membrane protein-1 (LMP1) of Epstein-Barr virus (EBV) was investigated in 153 cases of EBV+ classic Hodgkin lymphoma (cHL); 120 cases were pediatric patients (< 14 years of age) from Iraq, and 33 cases were adult patients from Italy. We describe for the first time the presence of LMP1 protein in EBV-encoded RNA (EBER)-negative follicular dendritic cells (FDCs) of reactive germinal centers (GC) associated with EBV+ cHL. Presence of LMP1+ GCs was independent of geographic region and age of patients. Variable numbers of reactive GCs were present in 22.2% of cases (34 of 153), whereas LMP1 staining of FDCs was present in about a third of cases (10 of 34) with reactive GC. Most cases with LMP1+ GC were mixed-cellularity (MC) subtype, but some nodular sclerosis (NS) was also present. GC cells with LMP1+ FDCs were surrounded by numerous EBV-infected cells which were positive for EBER, LMP1, and CD30. Double immunolocalization analysis revealed that LMP1 was associated with CD63, an exosomal marker, and with CD21. The possibility is discussed that peri-follicular EBV-infected cells release LMP1 protein, perhaps through exosomes, and that the protein is then captured by FDCs and is presented to EBER-negative GC B cells. Springer Berlin Heidelberg 2019-06-15 2019 /pmc/articles/PMC6647529/ /pubmed/31203443 http://dx.doi.org/10.1007/s00428-019-02605-w Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Uccini, Stefania
Al-Jadiry, Mazin F.
Pepe, Giuseppina
Pasquini, Anna
Alsaadawi, Adel R.
Al-Hadad, Salma A.
Di Napoli, Arianna
Tripodo, Claudio
Ruco, Luigi
Follicular dendritic cells display microvesicle-associated LMP1 in reactive germinal centers of EBV+ classic Hodgkin lymphoma
title Follicular dendritic cells display microvesicle-associated LMP1 in reactive germinal centers of EBV+ classic Hodgkin lymphoma
title_full Follicular dendritic cells display microvesicle-associated LMP1 in reactive germinal centers of EBV+ classic Hodgkin lymphoma
title_fullStr Follicular dendritic cells display microvesicle-associated LMP1 in reactive germinal centers of EBV+ classic Hodgkin lymphoma
title_full_unstemmed Follicular dendritic cells display microvesicle-associated LMP1 in reactive germinal centers of EBV+ classic Hodgkin lymphoma
title_short Follicular dendritic cells display microvesicle-associated LMP1 in reactive germinal centers of EBV+ classic Hodgkin lymphoma
title_sort follicular dendritic cells display microvesicle-associated lmp1 in reactive germinal centers of ebv+ classic hodgkin lymphoma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6647529/
https://www.ncbi.nlm.nih.gov/pubmed/31203443
http://dx.doi.org/10.1007/s00428-019-02605-w
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