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Switchable Nitroproteome States of Phytophthora infestans Biology and Pathobiology

The study demonstrates protein tyrosine nitration as a functional post-translational modification (PTM) in biology and pathobiology of the oomycete Phytophthora infestans (Mont.) de Bary, the most harmful pathogen of potato (Solanum tuberosum L.). Using two P. infestans isolates differing in their v...

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Autores principales: Izbiańska, Karolina, Floryszak-Wieczorek, Jolanta, Gajewska, Joanna, Gzyl, Jarosław, Jelonek, Tomasz, Arasimowicz-Jelonek, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6647872/
https://www.ncbi.nlm.nih.gov/pubmed/31379758
http://dx.doi.org/10.3389/fmicb.2019.01516
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author Izbiańska, Karolina
Floryszak-Wieczorek, Jolanta
Gajewska, Joanna
Gzyl, Jarosław
Jelonek, Tomasz
Arasimowicz-Jelonek, Magdalena
author_facet Izbiańska, Karolina
Floryszak-Wieczorek, Jolanta
Gajewska, Joanna
Gzyl, Jarosław
Jelonek, Tomasz
Arasimowicz-Jelonek, Magdalena
author_sort Izbiańska, Karolina
collection PubMed
description The study demonstrates protein tyrosine nitration as a functional post-translational modification (PTM) in biology and pathobiology of the oomycete Phytophthora infestans (Mont.) de Bary, the most harmful pathogen of potato (Solanum tuberosum L.). Using two P. infestans isolates differing in their virulence toward potato cv. Sarpo Mira we found that the pathogen generates reactive nitrogen species (RNS) in hyphae and mature sporangia growing under in vitro and in planta conditions. However, acceleration of peroxynitrite formation and elevation of the nitrated protein pool within pathogen structures were observed mainly during the avr P. infestans MP 946-potato interaction. Importantly, the nitroproteome profiles varied for the pathogen virulence pattern and comparative analysis revealed that vr MP 977 P. infestans represented a much more diverse quality spectrum of nitrated proteins. Abundance profiles of nitrated proteins that were up- or downregulated were substantially different also between the analyzed growth phases. Briefly, in planta growth of avr and vr P. infestans was accompanied by exclusive nitration of proteins involved in energy metabolism, signal transduction and pathogenesis. Importantly, the P. infestans-potato interaction indicated cytosolic RXLRs and Crinklers effectors as potential sensors of RNS. Taken together, we explored the first plant pathogen nitroproteome. The results present new insights into RNS metabolism in P. infestans indicating protein nitration as an integral part of pathogen biology, dynamically modified during its offensive strategy. Thus, the nitroproteome should be considered as a flexible element of the oomycete developmental and adaptive mechanism to different micro-environments, including host cells.
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spelling pubmed-66478722019-08-02 Switchable Nitroproteome States of Phytophthora infestans Biology and Pathobiology Izbiańska, Karolina Floryszak-Wieczorek, Jolanta Gajewska, Joanna Gzyl, Jarosław Jelonek, Tomasz Arasimowicz-Jelonek, Magdalena Front Microbiol Microbiology The study demonstrates protein tyrosine nitration as a functional post-translational modification (PTM) in biology and pathobiology of the oomycete Phytophthora infestans (Mont.) de Bary, the most harmful pathogen of potato (Solanum tuberosum L.). Using two P. infestans isolates differing in their virulence toward potato cv. Sarpo Mira we found that the pathogen generates reactive nitrogen species (RNS) in hyphae and mature sporangia growing under in vitro and in planta conditions. However, acceleration of peroxynitrite formation and elevation of the nitrated protein pool within pathogen structures were observed mainly during the avr P. infestans MP 946-potato interaction. Importantly, the nitroproteome profiles varied for the pathogen virulence pattern and comparative analysis revealed that vr MP 977 P. infestans represented a much more diverse quality spectrum of nitrated proteins. Abundance profiles of nitrated proteins that were up- or downregulated were substantially different also between the analyzed growth phases. Briefly, in planta growth of avr and vr P. infestans was accompanied by exclusive nitration of proteins involved in energy metabolism, signal transduction and pathogenesis. Importantly, the P. infestans-potato interaction indicated cytosolic RXLRs and Crinklers effectors as potential sensors of RNS. Taken together, we explored the first plant pathogen nitroproteome. The results present new insights into RNS metabolism in P. infestans indicating protein nitration as an integral part of pathogen biology, dynamically modified during its offensive strategy. Thus, the nitroproteome should be considered as a flexible element of the oomycete developmental and adaptive mechanism to different micro-environments, including host cells. Frontiers Media S.A. 2019-07-16 /pmc/articles/PMC6647872/ /pubmed/31379758 http://dx.doi.org/10.3389/fmicb.2019.01516 Text en Copyright © 2019 Izbiańska, Floryszak-Wieczorek, Gajewska, Gzyl, Jelonek and Arasimowicz-Jelonek. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Izbiańska, Karolina
Floryszak-Wieczorek, Jolanta
Gajewska, Joanna
Gzyl, Jarosław
Jelonek, Tomasz
Arasimowicz-Jelonek, Magdalena
Switchable Nitroproteome States of Phytophthora infestans Biology and Pathobiology
title Switchable Nitroproteome States of Phytophthora infestans Biology and Pathobiology
title_full Switchable Nitroproteome States of Phytophthora infestans Biology and Pathobiology
title_fullStr Switchable Nitroproteome States of Phytophthora infestans Biology and Pathobiology
title_full_unstemmed Switchable Nitroproteome States of Phytophthora infestans Biology and Pathobiology
title_short Switchable Nitroproteome States of Phytophthora infestans Biology and Pathobiology
title_sort switchable nitroproteome states of phytophthora infestans biology and pathobiology
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6647872/
https://www.ncbi.nlm.nih.gov/pubmed/31379758
http://dx.doi.org/10.3389/fmicb.2019.01516
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