Cargando…

CRISPRai for simultaneous gene activation and inhibition to promote stem cell chondrogenesis and calvarial bone regeneration

Calvarial bone healing remains difficult but may be improved by stimulating chondrogenesis of implanted stem cells. To simultaneously promote chondrogenesis and repress adipogenesis of stem cells, we built a CRISPRai system that comprised inactive Cas9 (dCas9), two fusion proteins as activation/repr...

Descripción completa

Detalles Bibliográficos
Autores principales:	Truong, Vu Anh, Hsu, Mu-Nung, Kieu Nguyen, Nuong Thi, Lin, Mei-Wei, Shen, Chih-Che, Lin, Chin-Yu, Hu, Yu-Chen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2019
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6648329/ https://www.ncbi.nlm.nih.gov/pubmed/30997496 http://dx.doi.org/10.1093/nar/gkz267

Ejemplares similares

Synthetic switch to minimize CRISPR off-target effects by self-restricting Cas9 transcription and translation
por: Shen, Chih-Che, et al.
Publicado: (2019)

Synthetic switch-based baculovirus for transgene expression control and selective killing of hepatocellular carcinoma cells
por: Lin, Mei-Wei, et al.
Publicado: (2018)

CRISPR-based Activation of Endogenous Neurotrophic Genes in Adipose Stem Cell Sheets to Stimulate Peripheral Nerve Regeneration
por: Hsu, Mu-Nung, et al.
Publicado: (2019)

S3norm: simultaneous normalization of sequencing depth and signal-to-noise ratio in epigenomic data
por: Xiang, Guanjue, et al.
Publicado: (2020)

Inducible expression of large gRNA arrays for multiplexed CRISPRai applications
por: Shaw, William M., et al.
Publicado: (2022)

Simultaneous precise editing of multiple genes in human cells
por: Riesenberg, Stephan, et al.
Publicado: (2019)

Quantitative and simultaneous translational control of distinct mammalian mRNAs
por: Endo, Kei, et al.
Publicado: (2013)

Simultaneous live imaging of the transcription and nuclear position of specific genes
por: Ochiai, Hiroshi, et al.
Publicado: (2015)

Simultaneous measurement of biochemical phenotypes and gene expression in single cells
por: Richer, Amanda L, et al.
Publicado: (2020)

Enhancing the efficiency of a PCR using gold nanoparticles
por: Li, Min, et al.
Publicado: (2005)

Author Correction: Inducible expression of large gRNA arrays for multiplexed CRISPRai applications
por: Shaw, William M., et al.
Publicado: (2023)

Simultaneous amplification and screening of whole plasmids using the T7 bacteriophage replisome
por: Xu, Yan, et al.
Publicado: (2006)

Simultaneous characterization of cellular RNA structure and function with in-cell SHAPE-Seq
por: Watters, Kyle E., et al.
Publicado: (2016)

Simultaneous measurement of passage through the restriction point and MCM loading in single cells
por: Håland, T. W., et al.
Publicado: (2015)

Simultaneous non-contiguous deletions using large synthetic DNA and site-specific recombinases
por: Krishnakumar, Radha, et al.
Publicado: (2014)

Simultaneous characterization of sense and antisense genomic processes by the double-stranded hidden Markov model
por: Glas, Julia, et al.
Publicado: (2016)

f-divergence cutoff index to simultaneously identify differential expression in the integrated transcriptome and proteome
por: Tang, Shaojun, et al.
Publicado: (2016)

A Novel method for the simultaneous identification of methylcytosine and hydroxymethylcytosine at a single base resolution
por: Kawasaki, Yuki, et al.
Publicado: (2017)

Simultaneous detection of microsatellite repeats and SNPs in the macrophage migration inhibitory factor (MIF) gene by thin-film biosensor chips and application to rural field studies
por: Zhong, Xiao-bo, et al.
Publicado: (2005)

USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products
por: Geu-Flores, Fernando, et al.
Publicado: (2007)

CRISPR/Cas9-based genome editing for simultaneous interference with gene expression and protein stability
por: Martínez, Virginia, et al.
Publicado: (2017)

Isocratic HPLC analysis for the simultaneous determination of dNTPs, rNTPs and ADP in biological samples
por: Ranjbarian, Farahnaz, et al.
Publicado: (2021)

Expanded MutaT7 toolkit efficiently and simultaneously accesses all possible transition mutations in bacteria
por: Mengiste, Amanuella A, et al.
Publicado: (2023)

Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci
por: Chen, Baohui, et al.
Publicado: (2016)

Simultaneous determination of ribonucleoside and deoxyribonucleoside triphosphates in biological samples by hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry
por: Kong, Ziqing, et al.
Publicado: (2018)

Simultaneous detection of mRNA transcription and decay intermediates by dual colour single mRNA FISH on subcellular resolution
por: Kramer, Susanne
Publicado: (2017)

A multiplexed bioluminescent reporter for sensitive and non-invasive tracking of DNA double strand break repair dynamics in vitro and in vivo
por: Chien, Jasper Che-Yung, et al.
Publicado: (2020)

Transgenic zebrafish model to study translational control mediated by upstream open reading frame of human chop gene
por: Lee, Hung-Chieh, et al.
Publicado: (2011)

A tractable method for simultaneous modifications to the head and tail of bacteriophage lambda and its application to enhancing phage-mediated gene delivery
por: Zanghi, Christine N., et al.
Publicado: (2007)

Methylation-Specific MLPA (MS-MLPA): simultaneous detection of CpG methylation and copy number changes of up to 40 sequences
por: Nygren, Anders O. H., et al.
Publicado: (2005)

Measuring nonhomologous end-joining, homologous recombination and alternative end-joining simultaneously at an endogenous locus in any transfectable human cell
por: Hussain, Suleman S, et al.
Publicado: (2021)

Terminal proteins of Streptomyces chromosome can target DNA into eukaryotic nuclei
por: Tsai, Hsiu-Hui, et al.
Publicado: (2008)

Fluorescence detection of single nucleotide polymorphisms using a universal molecular beacon
por: Lin, Yang-Wei, et al.
Publicado: (2008)

Emulsified BMVC derivative induced filtration for G-quadruplex DNA structural separation
por: Tsai, Yu-Lin, et al.
Publicado: (2011)

High-throughput assay and engineering of self-cleaving ribozymes by sequencing
por: Kobori, Shungo, et al.
Publicado: (2015)

Enhanced and prolonged baculovirus-mediated expression by incorporating recombinase system and in cis elements: a comparative study
por: Sung, Li-Yu, et al.
Publicado: (2013)

Selective ablation of 3′ RNA ends and processive RTs facilitate direct cDNA sequencing of full-length host cell and viral transcripts
por: Gallardo, Christian M, et al.
Publicado: (2022)

A sensitive array-based assay for identifying multiple TMPRSS2:ERG fusion gene variants
por: Lu, Qing, et al.
Publicado: (2008)

Interpretation of network-based integration from multi-omics longitudinal data
por: Bodein, Antoine, et al.
Publicado: (2021)

Identification of permissive amber suppression sites for efficient non-canonical amino acid incorporation in mammalian cells
por: Bartoschek, Michael D, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_k6j7vd0p4e785cr8nsnkli3r1h