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Raman-Based in Situ Monitoring of Changes in Molecular Signatures during Mitochondrially Mediated Apoptosis
[Image: see text] Obtaining molecular information from inside cells is an important topic to understand the outcome of molecular interactions between potential drug molecules and biomolecules inside cells. To envision this goal, we investigated the surface-enhanced Raman scattering-based single-cell...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6648662/ https://www.ncbi.nlm.nih.gov/pubmed/31459907 http://dx.doi.org/10.1021/acsomega.9b00629 |
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author | Shin, Hyeon Jeong Lee, Ji Hye Kim, Yong Duk Shin, Injae Sim, Taebo Lim, Dong-Kwon |
author_facet | Shin, Hyeon Jeong Lee, Ji Hye Kim, Yong Duk Shin, Injae Sim, Taebo Lim, Dong-Kwon |
author_sort | Shin, Hyeon Jeong |
collection | PubMed |
description | [Image: see text] Obtaining molecular information from inside cells is an important topic to understand the outcome of molecular interactions between potential drug molecules and biomolecules inside cells. To envision this goal, we investigated the surface-enhanced Raman scattering-based single-cell spectroscopic method to monitor changes in intracellular molecular signatures during mitochondrially mediated apoptosis in real time. Triphenylphosphine-modified gold nanoparticles were localized successfully to the mitochondria and greatly enhanced to obtain the intrinsic Raman scattering spectrum of mitochondria and cytochrome c in the live cell. Photothermally induced apoptosis showed a moderate decrease in the disulfide bond and a sharp increase in β-sheet structures depending on the input-laser power, along with morphological changes. However, chemical drug induced-apoptosis showed more subtle changes in the disulfide bond, as well as changes in Raman peaks corresponding to cytochrome c, and the appearance of a new peak at 1420 cm(–1), which enabled us to study the molecular interactions within the mitochondria in real time from a single cell, following treatment with a novel pyruvate dehydrogenase kinase inhibitor. |
format | Online Article Text |
id | pubmed-6648662 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-66486622019-08-27 Raman-Based in Situ Monitoring of Changes in Molecular Signatures during Mitochondrially Mediated Apoptosis Shin, Hyeon Jeong Lee, Ji Hye Kim, Yong Duk Shin, Injae Sim, Taebo Lim, Dong-Kwon ACS Omega [Image: see text] Obtaining molecular information from inside cells is an important topic to understand the outcome of molecular interactions between potential drug molecules and biomolecules inside cells. To envision this goal, we investigated the surface-enhanced Raman scattering-based single-cell spectroscopic method to monitor changes in intracellular molecular signatures during mitochondrially mediated apoptosis in real time. Triphenylphosphine-modified gold nanoparticles were localized successfully to the mitochondria and greatly enhanced to obtain the intrinsic Raman scattering spectrum of mitochondria and cytochrome c in the live cell. Photothermally induced apoptosis showed a moderate decrease in the disulfide bond and a sharp increase in β-sheet structures depending on the input-laser power, along with morphological changes. However, chemical drug induced-apoptosis showed more subtle changes in the disulfide bond, as well as changes in Raman peaks corresponding to cytochrome c, and the appearance of a new peak at 1420 cm(–1), which enabled us to study the molecular interactions within the mitochondria in real time from a single cell, following treatment with a novel pyruvate dehydrogenase kinase inhibitor. American Chemical Society 2019-05-06 /pmc/articles/PMC6648662/ /pubmed/31459907 http://dx.doi.org/10.1021/acsomega.9b00629 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Shin, Hyeon Jeong Lee, Ji Hye Kim, Yong Duk Shin, Injae Sim, Taebo Lim, Dong-Kwon Raman-Based in Situ Monitoring of Changes in Molecular Signatures during Mitochondrially Mediated Apoptosis |
title | Raman-Based in Situ Monitoring of Changes in Molecular
Signatures during Mitochondrially Mediated Apoptosis |
title_full | Raman-Based in Situ Monitoring of Changes in Molecular
Signatures during Mitochondrially Mediated Apoptosis |
title_fullStr | Raman-Based in Situ Monitoring of Changes in Molecular
Signatures during Mitochondrially Mediated Apoptosis |
title_full_unstemmed | Raman-Based in Situ Monitoring of Changes in Molecular
Signatures during Mitochondrially Mediated Apoptosis |
title_short | Raman-Based in Situ Monitoring of Changes in Molecular
Signatures during Mitochondrially Mediated Apoptosis |
title_sort | raman-based in situ monitoring of changes in molecular
signatures during mitochondrially mediated apoptosis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6648662/ https://www.ncbi.nlm.nih.gov/pubmed/31459907 http://dx.doi.org/10.1021/acsomega.9b00629 |
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