Rapid Fluorescent-Based Detection of New Delhi Metallo-β-Lactamases by Photo-Cross-Linking Using Conjugates of Azidonaphthalimide and Zinc(II)-Chelating Motifs

[Image: see text] A method for rapid detection of metallo-β-lactamases NDM-5 and NDM-7 using conjugates of azidonaphthalimide and Zn(II) chelating motifs (like sulfonamides, hydroxamate, and terpyridine) is described. Incubation and irradiation, followed by gel electrophoresis, clearly show the pres...

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Autores principales: Singha, Monisha, Kumar, Gaurav, Jain, Diamond, Kumar N, Ganesh, Ray, Debashis, Ghosh, Anindya S., Basak, Amit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6648899/
https://www.ncbi.nlm.nih.gov/pubmed/31460186
http://dx.doi.org/10.1021/acsomega.9b01145
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author Singha, Monisha
Kumar, Gaurav
Jain, Diamond
Kumar N, Ganesh
Ray, Debashis
Ghosh, Anindya S.
Basak, Amit
author_facet Singha, Monisha
Kumar, Gaurav
Jain, Diamond
Kumar N, Ganesh
Ray, Debashis
Ghosh, Anindya S.
Basak, Amit
author_sort Singha, Monisha
collection PubMed
description [Image: see text] A method for rapid detection of metallo-β-lactamases NDM-5 and NDM-7 using conjugates of azidonaphthalimide and Zn(II) chelating motifs (like sulfonamides, hydroxamate, and terpyridine) is described. Incubation and irradiation, followed by gel electrophoresis, clearly show the presence of NDMs. The o-sulfonamide-based probe has the highest efficiency of detection for both the NDMs. The proteins are detectable at nM concentrations, and the method is also selective, works both in vitro and in vivo, as revealed by cellular imaging and also with clinical isolates.
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spelling pubmed-66488992019-08-27 Rapid Fluorescent-Based Detection of New Delhi Metallo-β-Lactamases by Photo-Cross-Linking Using Conjugates of Azidonaphthalimide and Zinc(II)-Chelating Motifs Singha, Monisha Kumar, Gaurav Jain, Diamond Kumar N, Ganesh Ray, Debashis Ghosh, Anindya S. Basak, Amit ACS Omega [Image: see text] A method for rapid detection of metallo-β-lactamases NDM-5 and NDM-7 using conjugates of azidonaphthalimide and Zn(II) chelating motifs (like sulfonamides, hydroxamate, and terpyridine) is described. Incubation and irradiation, followed by gel electrophoresis, clearly show the presence of NDMs. The o-sulfonamide-based probe has the highest efficiency of detection for both the NDMs. The proteins are detectable at nM concentrations, and the method is also selective, works both in vitro and in vivo, as revealed by cellular imaging and also with clinical isolates. American Chemical Society 2019-06-21 /pmc/articles/PMC6648899/ /pubmed/31460186 http://dx.doi.org/10.1021/acsomega.9b01145 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Singha, Monisha
Kumar, Gaurav
Jain, Diamond
Kumar N, Ganesh
Ray, Debashis
Ghosh, Anindya S.
Basak, Amit
Rapid Fluorescent-Based Detection of New Delhi Metallo-β-Lactamases by Photo-Cross-Linking Using Conjugates of Azidonaphthalimide and Zinc(II)-Chelating Motifs
title Rapid Fluorescent-Based Detection of New Delhi Metallo-β-Lactamases by Photo-Cross-Linking Using Conjugates of Azidonaphthalimide and Zinc(II)-Chelating Motifs
title_full Rapid Fluorescent-Based Detection of New Delhi Metallo-β-Lactamases by Photo-Cross-Linking Using Conjugates of Azidonaphthalimide and Zinc(II)-Chelating Motifs
title_fullStr Rapid Fluorescent-Based Detection of New Delhi Metallo-β-Lactamases by Photo-Cross-Linking Using Conjugates of Azidonaphthalimide and Zinc(II)-Chelating Motifs
title_full_unstemmed Rapid Fluorescent-Based Detection of New Delhi Metallo-β-Lactamases by Photo-Cross-Linking Using Conjugates of Azidonaphthalimide and Zinc(II)-Chelating Motifs
title_short Rapid Fluorescent-Based Detection of New Delhi Metallo-β-Lactamases by Photo-Cross-Linking Using Conjugates of Azidonaphthalimide and Zinc(II)-Chelating Motifs
title_sort rapid fluorescent-based detection of new delhi metallo-β-lactamases by photo-cross-linking using conjugates of azidonaphthalimide and zinc(ii)-chelating motifs
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6648899/
https://www.ncbi.nlm.nih.gov/pubmed/31460186
http://dx.doi.org/10.1021/acsomega.9b01145
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