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Nitrogen, Cobalt Co-doped Fluorescent Magnetic Carbon Dots as Ratiometric Fluorescent Probes for Cholesterol and Uric Acid in Human Blood Serum

[Image: see text] Detection of cholesterol and uric acid biomarkers is of great importance for clinical diagnosis of several serious diseases correlated with their variations in human blood serum. In this study, a new kind of well selective and highly sensitive ratiometric fluorescent probe for chol...

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Autores principales: Huang, Shan, Yang, Erli, Yao, Jiandong, Chu, Xu, Liu, Yi, Zhang, Yue, Xiao, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6649034/
https://www.ncbi.nlm.nih.gov/pubmed/31460022
http://dx.doi.org/10.1021/acsomega.9b00874
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author Huang, Shan
Yang, Erli
Yao, Jiandong
Chu, Xu
Liu, Yi
Zhang, Yue
Xiao, Qi
author_facet Huang, Shan
Yang, Erli
Yao, Jiandong
Chu, Xu
Liu, Yi
Zhang, Yue
Xiao, Qi
author_sort Huang, Shan
collection PubMed
description [Image: see text] Detection of cholesterol and uric acid biomarkers is of great importance for clinical diagnosis of several serious diseases correlated with their variations in human blood serum. In this study, a new kind of well selective and highly sensitive ratiometric fluorescent probe for cholesterol and uric acid determination in human blood serum was innovatively developed on the basis of the inner filter effect (IFE) process of nitrogen, cobalt co-doped carbon dots (N,Co-CDs) with 2,3-diaminophenazine (DAP). DAP was the oxidative product during the oxidation reaction between o-phenylenediamine and H(2)O(2). Fluorescent magnetic N,Co-CDs possessing blue emission and magnetic property were prepared through a facile one-pot hydrothermal strategy by using citric acid, diethylenetriamine, and cobalt(II) chloride hexahydrate as precursors. N,Co-CDs exhibited good ferromagnetic property and excellent optical properties even in extremely harsh environmental conditions, implying the huge potential applications of such N,Co-CDs in biological areas. On the basis of the IFE process between N,Co-CDs and DAP, N,Co-CDs were applied to establish ratiometric fluorescent probes for the indirect detection of cholesterol and uric acid that participated in enzyme-catalyzed H(2)O(2)-generation reactions. The established IFE-based fluorescent probes exhibited relatively low detection limits of 3.6 nM for cholesterol and 3.4 nM for uric acid, respectively. The fluorescent probe was successfully utilized for the determination of cholesterol and uric acid in human blood serum with satisfying results, which provided an informed perspective on the applications of such doped CDs to explore the specific and sensitive nanoprobe in disease diagnoses and clinical therapy.
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spelling pubmed-66490342019-08-27 Nitrogen, Cobalt Co-doped Fluorescent Magnetic Carbon Dots as Ratiometric Fluorescent Probes for Cholesterol and Uric Acid in Human Blood Serum Huang, Shan Yang, Erli Yao, Jiandong Chu, Xu Liu, Yi Zhang, Yue Xiao, Qi ACS Omega [Image: see text] Detection of cholesterol and uric acid biomarkers is of great importance for clinical diagnosis of several serious diseases correlated with their variations in human blood serum. In this study, a new kind of well selective and highly sensitive ratiometric fluorescent probe for cholesterol and uric acid determination in human blood serum was innovatively developed on the basis of the inner filter effect (IFE) process of nitrogen, cobalt co-doped carbon dots (N,Co-CDs) with 2,3-diaminophenazine (DAP). DAP was the oxidative product during the oxidation reaction between o-phenylenediamine and H(2)O(2). Fluorescent magnetic N,Co-CDs possessing blue emission and magnetic property were prepared through a facile one-pot hydrothermal strategy by using citric acid, diethylenetriamine, and cobalt(II) chloride hexahydrate as precursors. N,Co-CDs exhibited good ferromagnetic property and excellent optical properties even in extremely harsh environmental conditions, implying the huge potential applications of such N,Co-CDs in biological areas. On the basis of the IFE process between N,Co-CDs and DAP, N,Co-CDs were applied to establish ratiometric fluorescent probes for the indirect detection of cholesterol and uric acid that participated in enzyme-catalyzed H(2)O(2)-generation reactions. The established IFE-based fluorescent probes exhibited relatively low detection limits of 3.6 nM for cholesterol and 3.4 nM for uric acid, respectively. The fluorescent probe was successfully utilized for the determination of cholesterol and uric acid in human blood serum with satisfying results, which provided an informed perspective on the applications of such doped CDs to explore the specific and sensitive nanoprobe in disease diagnoses and clinical therapy. American Chemical Society 2019-05-28 /pmc/articles/PMC6649034/ /pubmed/31460022 http://dx.doi.org/10.1021/acsomega.9b00874 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Huang, Shan
Yang, Erli
Yao, Jiandong
Chu, Xu
Liu, Yi
Zhang, Yue
Xiao, Qi
Nitrogen, Cobalt Co-doped Fluorescent Magnetic Carbon Dots as Ratiometric Fluorescent Probes for Cholesterol and Uric Acid in Human Blood Serum
title Nitrogen, Cobalt Co-doped Fluorescent Magnetic Carbon Dots as Ratiometric Fluorescent Probes for Cholesterol and Uric Acid in Human Blood Serum
title_full Nitrogen, Cobalt Co-doped Fluorescent Magnetic Carbon Dots as Ratiometric Fluorescent Probes for Cholesterol and Uric Acid in Human Blood Serum
title_fullStr Nitrogen, Cobalt Co-doped Fluorescent Magnetic Carbon Dots as Ratiometric Fluorescent Probes for Cholesterol and Uric Acid in Human Blood Serum
title_full_unstemmed Nitrogen, Cobalt Co-doped Fluorescent Magnetic Carbon Dots as Ratiometric Fluorescent Probes for Cholesterol and Uric Acid in Human Blood Serum
title_short Nitrogen, Cobalt Co-doped Fluorescent Magnetic Carbon Dots as Ratiometric Fluorescent Probes for Cholesterol and Uric Acid in Human Blood Serum
title_sort nitrogen, cobalt co-doped fluorescent magnetic carbon dots as ratiometric fluorescent probes for cholesterol and uric acid in human blood serum
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6649034/
https://www.ncbi.nlm.nih.gov/pubmed/31460022
http://dx.doi.org/10.1021/acsomega.9b00874
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