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Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis

We previously showed that dietary omega (ω)–3 long-chain polyunsaturated fatty acids (LCPUFAs) suppress inflammation in mice with experimental autoimmune uveitis (EAU). We have now investigated the role of antigen presenting cells (APCs) in this action of ω-3 LCPUFAs. C57BL/6 mice were fed a diet su...

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Autores principales: Uchi, Sho-Hei, Yanai, Ryoji, Kobayashi, Masaaki, Hatano, Makoto, Kobayashi, Yuka, Yamashiro, Chiemi, Nagai, Tomohiko, Tokuda, Kazuo, Connor, Kip M., Sonoda, Koh-Hei, Kimura, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650034/
https://www.ncbi.nlm.nih.gov/pubmed/31335861
http://dx.doi.org/10.1371/journal.pone.0219405
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author Uchi, Sho-Hei
Yanai, Ryoji
Kobayashi, Masaaki
Hatano, Makoto
Kobayashi, Yuka
Yamashiro, Chiemi
Nagai, Tomohiko
Tokuda, Kazuo
Connor, Kip M.
Sonoda, Koh-Hei
Kimura, Kazuhiro
author_facet Uchi, Sho-Hei
Yanai, Ryoji
Kobayashi, Masaaki
Hatano, Makoto
Kobayashi, Yuka
Yamashiro, Chiemi
Nagai, Tomohiko
Tokuda, Kazuo
Connor, Kip M.
Sonoda, Koh-Hei
Kimura, Kazuhiro
author_sort Uchi, Sho-Hei
collection PubMed
description We previously showed that dietary omega (ω)–3 long-chain polyunsaturated fatty acids (LCPUFAs) suppress inflammation in mice with experimental autoimmune uveitis (EAU). We have now investigated the role of antigen presenting cells (APCs) in this action of ω-3 LCPUFAs. C57BL/6 mice were fed a diet supplemented with ω-3 or ω-6 LCPUFAs for 2 weeks, after which splenocytes were isolated from the mice and cocultured with CD4(+) T cells isolated from mice with EAU induced by injection of a human interphotoreceptor retinoid-binding protein peptide together with complete Freund’s adjuvant. The proliferation of and production of interferon-γ and interleukin-17 by T cells from EAU mice in vitro were attenuated in the presence of splenocytes from ω-3 LCPUFA–fed mice as compared with those from mice fed ω-6 LCPUFAs. Splenocyte fractionation by magnetic-activated cell sorting revealed that, among APCs, dendritic cells (DCs) were the target of ω-3 LCPUFAs. Adoptive transfer of DCs from mice fed ω-3 LCPUFAs attenuated disease progression in EAU mice as well as the production of pro-inflammatory cytokines by T cells isolated from these latter animals. The proliferation of T cells from control Balb/c mice was also attenuated in the presence of DCs from ω-3 LCPUFA–fed mice as compared with those from ω-6 LCPUFA–fed mice. Furthermore, T cell proliferation in such a mixed lymphocyte reaction was inhibited by prior exposure of DCs from mice fed an ω-6 LCPUFA diet to ω-3 LCPUFAs in vitro. Our results thus suggest that DCs mediate the anti-inflammatory action of dietary ω-3 LCPUFAs in EAU.
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spelling pubmed-66500342019-07-25 Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis Uchi, Sho-Hei Yanai, Ryoji Kobayashi, Masaaki Hatano, Makoto Kobayashi, Yuka Yamashiro, Chiemi Nagai, Tomohiko Tokuda, Kazuo Connor, Kip M. Sonoda, Koh-Hei Kimura, Kazuhiro PLoS One Research Article We previously showed that dietary omega (ω)–3 long-chain polyunsaturated fatty acids (LCPUFAs) suppress inflammation in mice with experimental autoimmune uveitis (EAU). We have now investigated the role of antigen presenting cells (APCs) in this action of ω-3 LCPUFAs. C57BL/6 mice were fed a diet supplemented with ω-3 or ω-6 LCPUFAs for 2 weeks, after which splenocytes were isolated from the mice and cocultured with CD4(+) T cells isolated from mice with EAU induced by injection of a human interphotoreceptor retinoid-binding protein peptide together with complete Freund’s adjuvant. The proliferation of and production of interferon-γ and interleukin-17 by T cells from EAU mice in vitro were attenuated in the presence of splenocytes from ω-3 LCPUFA–fed mice as compared with those from mice fed ω-6 LCPUFAs. Splenocyte fractionation by magnetic-activated cell sorting revealed that, among APCs, dendritic cells (DCs) were the target of ω-3 LCPUFAs. Adoptive transfer of DCs from mice fed ω-3 LCPUFAs attenuated disease progression in EAU mice as well as the production of pro-inflammatory cytokines by T cells isolated from these latter animals. The proliferation of T cells from control Balb/c mice was also attenuated in the presence of DCs from ω-3 LCPUFA–fed mice as compared with those from ω-6 LCPUFA–fed mice. Furthermore, T cell proliferation in such a mixed lymphocyte reaction was inhibited by prior exposure of DCs from mice fed an ω-6 LCPUFA diet to ω-3 LCPUFAs in vitro. Our results thus suggest that DCs mediate the anti-inflammatory action of dietary ω-3 LCPUFAs in EAU. Public Library of Science 2019-07-23 /pmc/articles/PMC6650034/ /pubmed/31335861 http://dx.doi.org/10.1371/journal.pone.0219405 Text en © 2019 Uchi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Uchi, Sho-Hei
Yanai, Ryoji
Kobayashi, Masaaki
Hatano, Makoto
Kobayashi, Yuka
Yamashiro, Chiemi
Nagai, Tomohiko
Tokuda, Kazuo
Connor, Kip M.
Sonoda, Koh-Hei
Kimura, Kazuhiro
Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis
title Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis
title_full Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis
title_fullStr Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis
title_full_unstemmed Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis
title_short Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis
title_sort dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650034/
https://www.ncbi.nlm.nih.gov/pubmed/31335861
http://dx.doi.org/10.1371/journal.pone.0219405
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