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AGT haplotype in ITGA4 gene is related to antibody-mediated rejection in heart transplant patients
INTRODUCTION: One of the main problems involved in heart transplantation (HT) is antibody-mediated rejection (AMR). Many aspects of AMR are still unresolved, including its etiology, diagnosis and treatment. In this project, we hypothesize that variants in genes involved in B-cell biology in HT patie...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650139/ https://www.ncbi.nlm.nih.gov/pubmed/31335901 http://dx.doi.org/10.1371/journal.pone.0219345 |
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author | Núñez, Lucía Marrón-Liñares, Grecia M. Crespo-Leiro, María G. Barge-Caballero, Eduardo Álvarez-López, Eloy Suarez-Fuentetaja, Natalia Paniagua-Martin, María Jesús Pombo, Jorge Muñiz, Javier Tan, Carmela D. Rodríguez, E. René Vázquez-Rodríguez, José Manuel Hermida-Prieto, Manuel |
author_facet | Núñez, Lucía Marrón-Liñares, Grecia M. Crespo-Leiro, María G. Barge-Caballero, Eduardo Álvarez-López, Eloy Suarez-Fuentetaja, Natalia Paniagua-Martin, María Jesús Pombo, Jorge Muñiz, Javier Tan, Carmela D. Rodríguez, E. René Vázquez-Rodríguez, José Manuel Hermida-Prieto, Manuel |
author_sort | Núñez, Lucía |
collection | PubMed |
description | INTRODUCTION: One of the main problems involved in heart transplantation (HT) is antibody-mediated rejection (AMR). Many aspects of AMR are still unresolved, including its etiology, diagnosis and treatment. In this project, we hypothesize that variants in genes involved in B-cell biology in HT patients can yield diagnostic and prognostic information about AMR. METHODS: Genetic variants in 61 genes related to B-cell biology were analyzed by next generation sequencing in 46 HT patients, 23 with and 23 without AMR. RESULTS: We identified 3 single nucleotide polymorphisms in ITGA4 gene (c.1845G>A, c.2633A>G, and c.2883C>T) that conformed the haplotype AGT-ITGA4. This haplotype is associated with the development of AMR. Moreover, AMR patients with the haplotype AGT-ITGA4 present lower levels of integrin α-4 in serum samples compared to the reference GAC haplotype in control patients. CONCLUSION: We can conclude that polymorphisms in genes related to the biology of B-cells could have an important role in the development of AMR. In fact, the AGT haplotype in ITGA4 gene could potentially increase the risk of AMR. |
format | Online Article Text |
id | pubmed-6650139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-66501392019-07-25 AGT haplotype in ITGA4 gene is related to antibody-mediated rejection in heart transplant patients Núñez, Lucía Marrón-Liñares, Grecia M. Crespo-Leiro, María G. Barge-Caballero, Eduardo Álvarez-López, Eloy Suarez-Fuentetaja, Natalia Paniagua-Martin, María Jesús Pombo, Jorge Muñiz, Javier Tan, Carmela D. Rodríguez, E. René Vázquez-Rodríguez, José Manuel Hermida-Prieto, Manuel PLoS One Research Article INTRODUCTION: One of the main problems involved in heart transplantation (HT) is antibody-mediated rejection (AMR). Many aspects of AMR are still unresolved, including its etiology, diagnosis and treatment. In this project, we hypothesize that variants in genes involved in B-cell biology in HT patients can yield diagnostic and prognostic information about AMR. METHODS: Genetic variants in 61 genes related to B-cell biology were analyzed by next generation sequencing in 46 HT patients, 23 with and 23 without AMR. RESULTS: We identified 3 single nucleotide polymorphisms in ITGA4 gene (c.1845G>A, c.2633A>G, and c.2883C>T) that conformed the haplotype AGT-ITGA4. This haplotype is associated with the development of AMR. Moreover, AMR patients with the haplotype AGT-ITGA4 present lower levels of integrin α-4 in serum samples compared to the reference GAC haplotype in control patients. CONCLUSION: We can conclude that polymorphisms in genes related to the biology of B-cells could have an important role in the development of AMR. In fact, the AGT haplotype in ITGA4 gene could potentially increase the risk of AMR. Public Library of Science 2019-07-23 /pmc/articles/PMC6650139/ /pubmed/31335901 http://dx.doi.org/10.1371/journal.pone.0219345 Text en © 2019 Núñez et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Núñez, Lucía Marrón-Liñares, Grecia M. Crespo-Leiro, María G. Barge-Caballero, Eduardo Álvarez-López, Eloy Suarez-Fuentetaja, Natalia Paniagua-Martin, María Jesús Pombo, Jorge Muñiz, Javier Tan, Carmela D. Rodríguez, E. René Vázquez-Rodríguez, José Manuel Hermida-Prieto, Manuel AGT haplotype in ITGA4 gene is related to antibody-mediated rejection in heart transplant patients |
title | AGT haplotype in ITGA4 gene is related to antibody-mediated rejection in heart transplant patients |
title_full | AGT haplotype in ITGA4 gene is related to antibody-mediated rejection in heart transplant patients |
title_fullStr | AGT haplotype in ITGA4 gene is related to antibody-mediated rejection in heart transplant patients |
title_full_unstemmed | AGT haplotype in ITGA4 gene is related to antibody-mediated rejection in heart transplant patients |
title_short | AGT haplotype in ITGA4 gene is related to antibody-mediated rejection in heart transplant patients |
title_sort | agt haplotype in itga4 gene is related to antibody-mediated rejection in heart transplant patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650139/ https://www.ncbi.nlm.nih.gov/pubmed/31335901 http://dx.doi.org/10.1371/journal.pone.0219345 |
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